A5001945727- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- RNA modifications and cancer
- Obesity, Physical Activity, Diet
- Birth, Development, and Health
- Advanced Proteomics Techniques and Applications
- Hedgehog Signaling Pathway Studies
- Cancer Research and Treatments
- Single-cell and spatial transcriptomics
- vaccines and immunoinformatics approaches
- Youth, Drugs, and Violence
- Adrenal and Paraganglionic Tumors
- Pancreatic and Hepatic Oncology Research
- Effects of Radiation Exposure
- Graphene and Nanomaterials Applications
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- melanin and skin pigmentation
- Adipose Tissue and Metabolism
- Hormonal Regulation and Hypertension
- Melanoma and MAPK Pathways
Biocant
2025
Leiden University Medical Center
2019-2024
Universidade do Porto
2013-2022
Cornell University
2022
Weill Cornell Medicine
2022
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2016-2022
IPO Porto
2022
Rede de Química e Tecnologia
2022
Hospital da Luz
2019
Pancreatic ductal adenocarcinoma (PDAC) is notorious for its poor prognosis even after curative resection. Responses to immunotherapy are rare and related inadequate T-cell priming. We previously demonstrated the potency of allogeneic lysate-dendritic cell (DC) vaccination in a preclinical model. Here we translate this concept patients.In phase I study, patients with resected PDAC were included when they no radiologic signs recurrence standard-of-care treatment. Allogeneic tumour...
The integration of spatial omics technologies can provide important insights into the biology tissues. Here we combined mass spectrometry imaging-based metabolomics and imaging cytometry-based immunophenotyping on a single tissue section to reveal metabolic heterogeneity at single-cell resolution within tissues its association with specific cell populations such as cancer cells or immune cells. This approach has potential greatly increase our understanding tissue-level interplay between...
Group B Streptococcus (GBS) and Staphylococcus aureus cause 200.000 neonatal deaths every year no vaccine has been developed yet. Here, we described that extracellular glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from S. is an immunomodulatory protein. Antibody mediated neutralization of GAPDH promotes a protective inflammatory response by inhibiting early abnormal production IL-10 in infected mice. As role for was already GBS, selected peptides exposed on bacterial both bacteria but...
Mitochondria are central organelles for cellular metabolism. In cancer cells, mitochondrial oxidative phosphorylation (OXPHOS) dysfunction has been shown to promote migration, invasion, metastization and apoptosis resistance. With the purpose of analysing effects OXPHOS in cells molecular players involved, we generated cybrid cell lines harbouring either wild-type (WT) or mutant DNA (mtDNA) [tRNAmut cybrids, which harbour pathogenic A3243T mutation leucine transfer RNA gene (tRNAleu)]....
Abstract Radiobiology is moving towards a better understanding of the intercellular signaling that occurs upon radiation and how its effects relate to dose applied. The mitochondrial role in orchestrating this biological response needs be further explored. Cybrids (cytoplasmic hybrids) are useful cell models for studying involvement mitochondria cellular processes. In present study we used cybrid lines investigate exposure. Cybrid lines, derived from osteosarcoma human line 143B, harboring,...
Somatic mutations in GNAQ gene were described as being the main oncogenic activation uveal melanomas, whereas BRAF have been a key genetic alteration that contributes to skin melanoma development. We previously reported differential of MAPK and AKT/mTOR signalling pathways melanomas harbouring, respectively, mutations. The aim this work was compare functional effect mTOR pathway activation, cell proliferation apoptosis. In work, we performed transient transfection HEK293 cells with BRAF(WT),...
Abstract In recent years, significant attention has been given to “green” product innovation and related manufacturing processes. This work reports the preparation of few‐layer graphene (FLG) respective PLA‐based composites by an eco‐friendly, efficient, cost‐effective approach. FLG was produced in a scaled‐up process, based on noncovalent functionalization micronized graphite with pyrene derivate (PY), aqueous solution. The exfoliation degree, morphology, thermal stability exfoliated...
Abstract Integration of spatial omics technologies can provide important insights into the biology tissues. We combined mass spectrometry imaging-based metabolomics and imaging cytometry-based immunophenotyping on same single tissue section to reveal metabolic heterogeneity within tissues its association with specific cell populations like cancer cells or immune cells. This approach has potential greatly increase our understanding tissue-level interplay between processes their cellular components.
Transitions between epithelial and mesenchymal cellular states (EMT/MET) contribute to cancer progression. We hypothesize that EMT followed by MET promotes cell population heterogeneity, favouring tumour growth. developed an model on off exposure of EpH4 cells (E-cells) TGFβ1 mimics phenotypic (M-cells) MET. aimed at understanding whether is accompanied molecular functional reversion back epithelia using RNA sequencing, immunofluorescence (IF), proliferation, wound healing, focus formation...
<p>More detailed information on: 1) Immune-fluorescent staining, 2) Mass spectrometry, 3) MLTC and TIL cultures.</p>
<p>Expression of tumor associated antigens in early and later metastatic lesions.</p>
<p>Putative neoantigens in early and later metastatic lesions.</p>
<p>HLA class II expression and recognition of early later metastatic lesion- derived tumor cells from patient Mel5 by CD4+ CD8⁺ T cells.</p>
<p>Immunohistochemical staining of proteins involved in T cell recognition and/or inhibition.</p>
<p>HLA class I and II expression on melanoma cell lines derived from early later metastatic lesions.</p>
<p>T cell recognition of melanoma lines derived from early and later metastatic lesions.</p>