R.A.H. Timmermans

ORCID: 0000-0003-4020-1715
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Research Areas
  • Microbial Inactivation Methods
  • Listeria monocytogenes in Food Safety
  • Food Drying and Modeling
  • Magnetic and Electromagnetic Effects
  • SARS-CoV-2 and COVID-19 Research
  • COVID-19 Impact on Reproduction
  • Radiation Effects and Dosimetry
  • Infant Nutrition and Health
  • Advanced Chemical Sensor Technologies
  • Ultrasound and Cavitation Phenomena
  • Neurobiology and Insect Physiology Research
  • Insect and Arachnid Ecology and Behavior
  • Probiotics and Fermented Foods
  • Transgenic Plants and Applications
  • Vaccine Coverage and Hesitancy
  • 3D Printing in Biomedical Research
  • Meat and Animal Product Quality
  • biodegradable polymer synthesis and properties
  • Insect Utilization and Effects
  • Coagulation and Flocculation Studies
  • Algal biology and biofuel production
  • Breastfeeding Practices and Influences
  • Viral gastroenteritis research and epidemiology
  • Postharvest Quality and Shelf Life Management
  • Microbial Metabolites in Food Biotechnology

Wageningen University & Research
2011-2024

Moderate intensity Pulsed Electric Fields (PEF) was studied for microbial inactivation as an alternative to high PEF or classical thermal pasteurization. The process is characterized by the application of electric pulses, allowing increase product temperature ohmic heat generated pulses. A systematic evaluation effect parameters field strength (E) and pulse width (τ) on Escherichia coli, Listeria monocytogenes, Lactobacillus plantarum, Salmonella Senftenberg Saccharomyces cerevisiae in...

10.1016/j.ijfoodmicro.2019.02.015 article EN cc-by-nc-nd International Journal of Food Microbiology 2019-03-18

Pulsed Electric Field (PEF) is currently discussed as promising technology for mild and scalable cell disintegration of microalgae. In this study Chlorella vulgaris Neochloris oleoabundans have been subjected to batch continuous PEF treatments under a wide range operating conditions (1–40 pulses, 0.05–5 ms 7.5–30 kV cm− 1, 0.05–150 kWh kgDW− 1). many cases after treatment, both algal species show release ions, which indicates that treatment resulted in permeabilization the cell. However,...

10.1016/j.algal.2017.03.024 article EN cc-by Algal Research 2017-04-01

Background: Since the outbreak of coronavirus disease 2019 (COVID-19), many put their hopes in rapid availability effective immunizations. Human milk, containing antibodies against syndrome 2 (SARS-CoV-2), may serve as means protection through passive immunization. We aimed to determine presence and pseudovirus neutralization capacity SARS-CoV-2 specific IgA human milk mothers who recovered from COVID-19, effect pasteurization on these antibodies. Methods: This prospective case control study...

10.3390/nu13051645 article EN Nutrients 2021-05-13

Insect production currently involves thermal processes to ensure microbial safety and inactivate endogenous enzymes, but these negatively affect the protein's technological properties. Therefore, this study compared high hydrostatic pressure (HHP) at 200/400/600 MPa for 10 min blanching 90 °C min, on soluble protein fractions (pH 3) from house crickets (Acheta domesticus) lesser mealworms (Alphitobius diaperinus). HHP significantly reduced load, especially 400 600 MPa. For aerobic counts of...

10.1016/j.ifset.2024.103743 article EN cc-by Innovative Food Science & Emerging Technologies 2024-07-04

Novel pulsed electric field (PEF) process conditions at moderate strength and long pulse duration have recently been established to obtain microbial inactivation. In this study, the effect of these PEF (E = 0.9 2.7 kV/cm, with 1000 µs) variable maximum temperatures was evaluated on quality attributes freshly squeezed orange juice. Results were compared juice that received no treatment or a mild severe thermal pasteurization treatment. No differences for pH soluble solids found after...

10.3390/foods11213360 article EN cc-by Foods 2022-10-25

Abstract Pulsed electric field (PEF) treatment, or electroporation, can be used to load molecules into cells. The permeabilizing effect of the PEF treatment on cellular membrane either reversible irreversible depending severity conditions. influence reversibility permeabilization in Lactobacillus plantarum WCFS1 by two different fluorescent staining methods was investigated this study. Whereas with propidium iodide (PI) before and after indicated small permeabilized fractions maximum 14%,...

10.1038/s41598-019-56299-w article EN cc-by Scientific Reports 2019-12-27

Abstract Background Since the outbreak of COVID-19, many put their hopes in rapid development effective immunizations. For now patient isolation, physical distancing and good hygiene are sole measures for prevention. Processed breast milk with antibodies against SaRS-CoV-2 may serve as additional protection. We aimed to determine presence neutralization capacity breastmilk mothers who have recovered from COVID-19. Methods This prospective case control study included lactating mothers,...

10.1101/2020.08.18.20176743 preprint EN medRxiv (Cold Spring Harbor Laboratory) 2020-08-21

Holder pasteurization (HoP) is the current recommended treatment for donor human milk. Although this method inactivates microbial contaminants, it also negatively affects various milk components. High-pressure processing (HPP, 400, 500, and 600 MPa), ultraviolet-C irradiation (UV-C, 2,430, 3,645, 4,863 J/L) thermoultrasonication (TUS, 1,080 1,620 kJ/L) were investigated as alternatives to thermal (HoP). We assessed effects of these methods on microbiological safety, concentration...

10.3389/fped.2022.828448 article EN cc-by Frontiers in Pediatrics 2022-03-21

High pressure processing is a mild preservation process that inactivates pathogenic and spoilage micro-organisms in food products, but preserves the fresh characteristics of product. Compared to untreated product, an enhanced shelf life obtained during refrigerated storage. Knowledge on use high pasteurisation aimed for ambient storage limited. The aim this research was investigate if combination moderate heat could be used produce shelf-stable high-acid fruit Ascospores resistant fungi...

10.1016/j.ijfoodmicro.2020.108629 article EN cc-by-nc-nd International Journal of Food Microbiology 2020-04-13

Since the outbreak of coronavirus disease 2019 (COVID-19), many put their hopes in rapid development effective immunizations. As this intervention will not be available on a short notice, patient isolation, physical distancing, protective materials and good hygiene are currently sole measures for prevention. Here we provide multiple lines evidence presence variety antibodies against severe acute respiratory syndrome 2 (SARS-CoV-2) present breastmilk from mothers who have recovered COVID-19,...

10.2139/ssrn.3633123 article EN SSRN Electronic Journal 2020-01-01

Pulsed electric field (PEF) treatment can be used to increase intracellular small molecule concentrations in bacteria, which lead enhanced robustness of these cells during further processing. In this study we investigated the effects PEF temperature and presence 8% (v/v) ethanol medium on cell survival, membrane fluidity trehalose Lactobacillus plantarum WCFS1. A moderate 21 °C resulted a high survival combined with higher compared at 10 35 °C. Interestingly, highest were observed upon...

10.1016/j.bioelechem.2020.107680 article EN cc-by-nc-nd Bioelectrochemistry 2020-10-01
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