A5069515239- Neuroinflammation and Neurodegeneration Mechanisms
- Long-Term Effects of COVID-19
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Protein Degradation and Inhibitors
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- COVID-19 and Mental Health
- Multiple Sclerosis Research Studies
- Ubiquitin and proteasome pathways
- Viral Infections and Vectors
- Virology and Viral Diseases
- Botulinum Toxin and Related Neurological Disorders
- Multiple Myeloma Research and Treatments
- Inflammasome and immune disorders
- Bacteriophages and microbial interactions
- Parkinson's Disease Mechanisms and Treatments
- Neurological diseases and metabolism
Austral University of Chile
2020-2024
Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson's disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote activation. Using transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) as COVID-19 pre-clinical model,...
Despite unprecedented global efforts to rapidly develop SARS-CoV-2 treatments, in order reduce the burden placed on health systems, situation remains critical. Effective diagnosis, treatment, and prophylactic measures are urgently required meet demand: recombinant antibodies fulfill these requirements have marked clinical potential. Here, we describe fast-tracked development of an alpaca Nanobody specific for receptor-binding-domain (RBD) Spike protein with potential therapeutic...
Nipah virus (NiV) and Hendra (HeV) are highly pathogenic henipaviruses without approved human vaccines or therapies. Here, we report on a potent bispecific therapeutic that combines an anti-fusion (F) nanobody with anti-receptor binding protein (RBP) antibody to deliver dual-targeting biologic is resistant viral escape. We show the nanobody, DS90, engages unique, conserved site within prefusion F of NiV HeV, provides neutralization complete protection from disease. Bispecific engineering...
The COVID-19 pandemic has driven biotechnological developments to provide new and more effective tools for prophylaxis, diagnosis, therapy. Historically, monoclonal antibodies have been valuable tools; however, the shown some weaknesses, such as production limitations at a global scale. An alternative conventional are nanobodies, recombinant fragments of variable region single-domain derived mainly from Camelidae family. Nanobodies multiple characteristic benefits: they small (15 KDa)...
ABSTRACT Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson’s disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote utilising model human monocyte-derived microglia. We identified isolates bind and enter microglia, triggering in...
Abstract The p97 protein is a member of the AAA + family ATPases. It mechanoenzyme that uses energy from ATP hydrolysis to promote unfolding and segregation actively. unfolded products are presented 26S Proteasome for degradation. substrate recognition mediated by adaptors, which interact with substrates directly or indirectly through ubiquitin modifications, resulting in funnelling into central pore hexamer unfolding. We have engineered synthetic adaptors target specific p97, using...
Proteinaceous inclusions, called Lewy bodies (LBs), are used as a pathological hallmark for Parkinson's disease (PD). Recent studies suggested prion-like spreading mechanism α-synucleinopathy where early neuropathological deposits occur, among others, in the olfactory bulb (OB) and amygdala. LBs contain insoluble α-synuclein many other ubiquitinated proteins, suggesting role of protein degradation system failure PD pathogenesis. Therefore, we wanted to study effects proteasomal inhibitor,...