A5009574816- Helminth infection and control
- SARS-CoV-2 and COVID-19 Research
- Parasitic Infections and Diagnostics
- Irrigation Practices and Water Management
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Parasitic infections in humans and animals
- vaccines and immunoinformatics approaches
- Marine Bivalve and Aquaculture Studies
- Microbial infections and disease research
- Greenhouse Technology and Climate Control
- Mollusks and Parasites Studies
- Aquaculture Nutrition and Growth
- Coccidia and coccidiosis research
- Plant Water Relations and Carbon Dynamics
- Marine and fisheries research
- Protein Degradation and Inhibitors
- Environmental Toxicology and Ecotoxicology
- Lepidoptera: Biology and Taxonomy
- Fish Ecology and Management Studies
- Autophagy in Disease and Therapy
- Peptidase Inhibition and Analysis
- Ubiquitin and proteasome pathways
- Mercury impact and mitigation studies
- Plant and animal studies
San Sebastián University
2024-2025
Austral University of Chile
2010-2024
Centro de Estudios Científicos
2024
California State University, Fresno
2021-2022
Center for Interdisciplinary Studies
2022
Clovis Community College
2021
Agriaquaculture Nutritional Genomic Center
2017
University of Concepción
2006
Aging is a major risk factor for neurodegenerative diseases, and coronavirus disease 2019 (COVID-19) linked to severe neurological manifestations. Senescent cells contribute brain aging, but the impact of virus-induced senescence on neuropathologies unknown. Here we show that senescent accumulate in aged human organoids senolytics reduce age-related inflammation rejuvenate transcriptomic aging clocks. In postmortem brains patients with COVID-19 observed increased cell accumulation compared...
Despite unprecedented global efforts to rapidly develop SARS-CoV-2 treatments, in order reduce the burden placed on health systems, situation remains critical. Effective diagnosis, treatment, and prophylactic measures are urgently required meet demand: recombinant antibodies fulfill these requirements have marked clinical potential. Here, we describe fast-tracked development of an alpaca Nanobody specific for receptor-binding-domain (RBD) Spike protein with potential therapeutic...
Parasitic infections pose a significant global health burden, affecting millions of people worldwide. Despite their importance, studies integrating human and animal parasitology to understand transmission pathways are scarce. This study, conducted between August 2022 April 2023, aimed investigate the prevalence parasites in humans domestic dogs Los Rios Region, southern Chile, explore risk factors associated with parasitism. A total 291 participants provided fecal blood samples for...
Nipah virus (NiV) and Hendra (HeV) are highly pathogenic henipaviruses without approved human vaccines or therapies. Here, we report on a potent bispecific therapeutic that combines an anti-fusion (F) nanobody with anti-receptor binding protein (RBP) antibody to deliver dual-targeting biologic is resistant viral escape. We show the nanobody, DS90, engages unique, conserved site within prefusion F of NiV HeV, provides neutralization complete protection from disease. Bispecific engineering...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) Omicron variant sub-lineages spread rapidly worldwide, mostly due to their immune-evasive properties. This has put a significant part of the population at risk for disease and underscores need effective anti-SARS-CoV-2 agents against emergent strains in vulnerable patients. Camelid nanobodies are attractive therapeutic candidates high stability, ease large-scale production, potential delivery via inhalation. Here, we...
The COVID-19 pandemic has driven biotechnological developments to provide new and more effective tools for prophylaxis, diagnosis, therapy. Historically, monoclonal antibodies have been valuable tools; however, the shown some weaknesses, such as production limitations at a global scale. An alternative conventional are nanobodies, recombinant fragments of variable region single-domain derived mainly from Camelidae family. Nanobodies multiple characteristic benefits: they small (15 KDa)...
Ubiquitination regulates several biological processes, however the role of specific members ubiquitinome on intracellular membrane trafficking is not yet fully understood. Here, we search for ubiquitin-related genes implicated in protein performing a High-Content siRNA Screening including 1187 human “ubiquitinome” using amyloid precursor (APP) as reporter. We identified deubiquitinating enzyme PSMD14, subunit 19S regulatory particle proteasome, K63-Ub chains cells, novel regulator...
Abstract Despite unprecedented global efforts to rapidly develop SARS-CoV-2 treatments, in order reduce the burden placed on health systems, situation remains critical. Effective diagnosis, treatment, and prophylactic measures are urgently required meet demand: recombinant antibodies fulfill these requirements have marked clinical potential. Here, we describe fast-tracked development of an alpaca Nanobody specific for receptor-binding-domain (RBD) Spike protein with therapeutic potential...
Autophagy engulfs cellular components in double-membrane-bound autophagosomes for clearance and recycling after fusion with lysosomes. Thus, autophagy is a key process maintaining proteostasis powerful cell-intrinsic host defense mechanism, protecting cells against pathogens by targeting them through specific form of selective known as xenophagy. In this context, ubiquitination acts signal recognition the cargoes autophagic receptors, which direct towards subsequent breakdown. Nevertheless,...
The influence of circulation on abundance patterns larval fish was compared at different phases the tide in vicinity an estuarine front Corral Bay, Chile during austral spring. Greatest differences water salinity were found across frontal region ebb tides. Because rainfall low, density primarily due to tidal intrusion salt water. Larval relatively low both sampling periods with Strangomera bentincki , Oodntesthes regia laticlavia Gobiesox marmoratus and Hypsoblennius sordidus as most...
Con el objeto de conocer la epidemiologia nematodos Trichostrongilidos en alpacas (Lama pacos) Sur Chile, se realizo un estudio predio Teja Norte ubicado comuna Valdivia (39° 48 S, 73° 14 W) mediante examenes material fecal (tecnicas Mc Master y Sedimentacion-Flotacion) pasto (tecnica Parffit); frecuencia muestreo fue mensual, durante 15 meses a partir enero 1995. Se utilizaron 47 animales, numero de: 4 (Grupo A, < 1 ano), 11 B, 1-2 anos) 32 C, 2 anos). Los generos o especies larvas...
Mortalidad natural de
Abstract The p97 protein is a member of the AAA + family ATPases. It mechanoenzyme that uses energy from ATP hydrolysis to promote unfolding and segregation actively. unfolded products are presented 26S Proteasome for degradation. substrate recognition mediated by adaptors, which interact with substrates directly or indirectly through ubiquitin modifications, resulting in funnelling into central pore hexamer unfolding. We have engineered synthetic adaptors target specific p97, using...
Abstract Infectious pancreatic necrosis virus (IPNv) poses a significant threat to the global salmon farming industry, causing high mortality and economic losses. Given limitations of traditional antibody therapies in aquaculture, we explored development nanobodies targeting VP2 protein IPNv. We developed novel nanobody library generation method using streamlined assembly protocol based on Type IIS restriction enzymes. This enabled quick efficient bacterial display generation, concatenated,...
The anatomical distribution and viability of cysticerci Taenia saginata in naturally infected cattle slaughtered southern Chile was determined.The were inspected post mortem according to official chilean procedures, where mandatory inspection sites are: heart, tongue, masseter, oesophagus, diaphragm superficial observation the carcass.Twenty carcasses diagnosed as positive during chosen at random their left half sides separated into 33 meat cuts; these completely dissected approximately 0.5...
This study aims to provide a quantitative analysis of the amino acids (AAs) and fatty (FAs) found in by-products Patagonian marine polychaete Abarenicola pusilla. Samples polychaetes were taken different seasons processed generate three by-products: eviscerated whole body, press cake, liquid. The results showed clear seasonal difference, with essential nutrients decreasing significantly winter. For example, from body specimens, acids, eicosapentaenoic acid (EPA), arachidonic (ARA) maintained...
Se realizo un estudio de desarrollo huevos Fasciola hepatica en el medio ambiente Temuco, Novena Region Chile (38-41S y 72-25W). Las observaciones se realizaron desde abril 1988 hasta junio 1990. Los obtenidos vesiculas bovinos infectados con parasito fueron colocados una vez al mes vasos agua destilada, sistema flotante. El flotante mantuvo canales, habitat del parasito. muestras ueron examinadas obtener 50% los eclosionados. tiempo mas largo fue 249 dias las colocadas corto, 37 dias, enero...
Here we show the cloning and characterization of a novel homolog prepro C-RFa cDNA from Cyprinus carpio.The deduced preprohormone precursor 115 amino acids leads to mature bioactive peptide 20 with identical sequence other teleost C-RFa.Modeling highlighted signifi cant similarity homologous human PrRP20, specifi cally conserved amphipathic system defi ned by C-terminal alpha-helix.Clearly, synthetic stimulated prolactin release primary cultured fi sh pituitary cells.For rst time, variation...
For the first time in Chile, cases of nymphs brown dog tick (Rhipicephalus sanguineus) being found on two goats (Capra hircus), and adult (one male a gravid female) white stripped ticks (Amblyomma tigrinum) cattle (Bos taurus) have been reported.The aim this review is to discuss importance these findings.