A5083722971- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Telomeres, Telomerase, and Senescence
- Circadian rhythm and melatonin
- HIV Research and Treatment
- Extracellular vesicles in disease
- Viral Infections and Outbreaks Research
- Insect symbiosis and bacterial influences
- Neuroinflammation and Neurodegeneration Mechanisms
- Malaria Research and Control
- Long-Term Effects of COVID-19
- interferon and immune responses
- CCD and CMOS Imaging Sensors
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
The University of Queensland
2019-2024
Abstract The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite large size of viral RNA genome (~30 kb), infectious full-length cDNA readily assembled in vitro a circular polymerase extension reaction (CPER) methodology without need for technically demanding intermediate steps. Overlapping fragments are generated from and together with linker fragment containing CMV promoter into single reaction. Transfection...
Two antibodies against flaviviruses Flaviviruses are a group of RNA viruses that include the human pathogens dengue virus, Zika and West Nile virus. The envelope protein (E) on virus surface has been target vaccine development, but problems have arisen with E, leading to enhanced infection. Now, Modhiran et al. Biering describe two different bind flavivirus NS1 prevent it from disrupting epithelial cells, which is associated severe disease. Both cross-react multiple proteins. reduce viremia...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 and is capable human-to-human transmission rapid global spread. The emergence spread SARS-CoV-2 encouraged establishment a rapid, sensitive, reliable viral detection quantification methodology. Here, we present an alternative assay, termed immuno-plaque assay (iPA), which utilizes combination plaque immunofluorescence techniques. We have extensively optimized...
Aging is a major risk factor for neurodegenerative diseases, and coronavirus disease 2019 (COVID-19) linked to severe neurological manifestations. Senescent cells contribute brain aging, but the impact of virus-induced senescence on neuropathologies unknown. Here we show that senescent accumulate in aged human organoids senolytics reduce age-related inflammation rejuvenate transcriptomic aging clocks. In postmortem brains patients with COVID-19 observed increased cell accumulation compared...
Abstract Flaviviruses, including Zika virus (ZIKV), utilise host mRNA degradation machinery to produce subgenomic flaviviral RNA (sfRNA). In mammalian hosts, this noncoding facilitates replication and pathogenesis of flaviviruses by inhibiting IFN-signalling, whereas the function sfRNA in mosquitoes remains largely elusive. Herein, we conduct a series vitro vivo experiments define role ZIKV infected Aedes aegypti employing viruses deficient production sfRNA. We show that sfRNA-deficient have...
All flaviviruses, including Zika virus, produce noncoding subgenomic flaviviral RNA (sfRNA), which plays an important role in viral pathogenesis. However, the exact mechanism of how sfRNA enables evasion antiviral response is not well defined. Here, we show that required for transplacental virus dissemination pregnant mice and subsequent fetal brain infection. We also promotes apoptosis neural progenitor cells human organoids, leading to their disintegration. In infected placental cells,...
Abstract In 2022, a genotype IV (GIV) strain of Japanese encephalitis virus (JEV) caused an unprecedented and widespread outbreak disease in pigs humans Australia. As no veterinary vaccines against JEV are approved Australia all current human derived from (G) III strains, we used the recently described insect-specific Binjari (BinJV) chimeric flavivirus vaccine technology to produce GIV candidate. Herein describe production displaying structural prM E proteins isolate obtained stillborn...
The COVID-19 pandemic is the biggest public health threat facing world today. Multiple vaccines have been approved; however, emergence of viral variants such as recent Omicron raises possibility booster doses to achieve adequate protection. In Brazil, CoronaVac (Sinovac, Beijing, China) vaccine was used; it important assess immune response this over time. This study aimed monitor anti-SARS-CoV-2 antibody responses in those immunized with and SARS-CoV-2 infected individuals. Samples were...
Abstract Aging is the primary risk factor for most neurodegenerative diseases, and recently coronavirus disease 2019 (COVID-19) has been associated with severe neurological manifestations that can eventually impact conditions in long-term. The progressive accumulation of senescent cells vivo strongly contributes to brain aging co-morbidities but virus-induced senescence aetiology neuropathologies unknown. Here, we show accumulate physiologically aged organoids human origin senolytic...
Abstract Aging is the primary risk factor for most neurodegenerative diseases, and recently coronavirus disease 2019 (COVID-19) has been associated with severe neurological manifestations that can eventually impact conditions in long-term. The progressive accumulation of senescent cells vivo strongly contributes to brain aging co-morbidities but virus-induced senescence aetiology neuropathologies unknown. Here, we show accumulate physiologically aged organoids human origin senolytic...
Similar to other flaviviruses, Zika virus (ZIKV) produces abundant subgenomic flavivirus RNA (sfRNA) derived from the 3' untranslated region. The molecular mechanisms that determine functions of sfRNA are currently not completely understood. Here, we created ZIKV mutants deficient in production and employed them investigate role this interactions with mammalian insect hosts. We found mosquitoes, facilitates replication is required for dissemination into saliva transmission. was have no...
Abstract Zika virus (ZIKV) is a re-emerging pathogenic flavivirus, which causes microcephaly in infants and poses continuing threat to public health. ZIKV, like all other flaviviruses, produces highly abundant noncoding RNA known as subgenomic flaviviral (sfRNA). Herein we utilized wild-type mutant ZIKV defective production of sfRNA elucidate for the first time how affects aspects pathogenesis. We found that mouse pregnancy model infection required trans-placental dissemination subsequent...
The COVID-19 pandemic is the biggest public health threat facing globe today. Multiple vaccines have been approved, however emergence of viral variants such as recent Omicron, raises possibility booster doses to achieve adequate protection. In Brazil, CoronaVac (Sinovac) vaccine was used, it’s important assess immune response this over time. This study aimed monitor anti-SARS-CoV-2 antibody responses in those immunized with and SARS-CoV-2 infected individuals. Samples were...
Abstract In 2022, a genotype IV (GIV) strain of Japanese encephalitis virus caused an unprecedented and widespread outbreak disease in pigs humans Australia. As no veterinary vaccines against JEV are approved Australia all current human derived from (G) III strains, we used the recently described insect-specific Binjari (BinJV) chimeric flavivirus vaccine technology to produce GIV candidate. Herein describe production displaying structural prM E proteins isolate obtained stillborn piglet...