A5072578794- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Glutathione Transferases and Polymorphisms
- Extracellular vesicles in disease
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Genomics, phytochemicals, and oxidative stress
- Zoonotic diseases and public health
- Virology and Viral Diseases
- Circular RNAs in diseases
- Neonatal and fetal brain pathology
- Aquaculture disease management and microbiota
- Malaria Research and Control
- Herpesvirus Infections and Treatments
- Pregnancy and preeclampsia studies
- Axon Guidance and Neuronal Signaling
- Long-Term Effects of COVID-19
- Epigenetics and DNA Methylation
- vaccines and immunoinformatics approaches
- Amphibian and Reptile Biology
- Agriculture and Biological Studies
- Vector-borne infectious diseases
The University of Queensland
2014-2024
Australian e-Health Research Centre
2023
Institute of Molecular Biology and Genetics
2009-2010
National Academy of Sciences of Ukraine
2010
Why individuals with Down syndrome (DS) are more susceptible to SARS-CoV-2–induced neuropathology remains elusive. Choroid plexus (ChP) plays critical roles in barrier function and immune response modulation expresses the ACE2 receptor chromosome 21–encoded TMPRSS2 protease, suggesting its substantial role establishing SARS-CoV-2 infection brain. To explore this, we established brain organoids from DS isogenic euploid iPSC that consist of a core functional cortical neurons surrounded by...
West Nile virus (WNV) is an enveloped with a single-stranded positive-sense RNA genome from the Flaviviridae family. WNV spread by mosquitoes and able to infect humans, causing encephalitis meningitis that can be fatal; it therefore presents significant risk for human health. In insects, innate response infection mostly relies on interference JAK/SAT pathways; however, some evidence indicates also involve microRNAs (miRNAs). miRNAs are small noncoding RNAs regulate gene expression at...
ABSTRACT West Nile virus (WNV) is a mosquito-transmitted flavivirus that naturally circulates between mosquitos and birds but can also infect humans, causing severe neurological disease. The early host response to WNV infection in vertebrates primarily relies on the type I interferon pathway; however, recent studies suggest microRNAs (miRNAs) may play notable role. In this study, we assessed role of miRNAs human cells. We employed small RNA sequencing (RNA-seq) analysis determine changes...
Abstract Flaviviruses, including Zika virus (ZIKV), utilise host mRNA degradation machinery to produce subgenomic flaviviral RNA (sfRNA). In mammalian hosts, this noncoding facilitates replication and pathogenesis of flaviviruses by inhibiting IFN-signalling, whereas the function sfRNA in mosquitoes remains largely elusive. Herein, we conduct a series vitro vivo experiments define role ZIKV infected Aedes aegypti employing viruses deficient production sfRNA. We show that sfRNA-deficient have...
All flaviviruses, including Zika virus, produce noncoding subgenomic flaviviral RNA (sfRNA), which plays an important role in viral pathogenesis. However, the exact mechanism of how sfRNA enables evasion antiviral response is not well defined. Here, we show that required for transplacental virus dissemination pregnant mice and subsequent fetal brain infection. We also promotes apoptosis neural progenitor cells human organoids, leading to their disintegration. In infected placental cells,...
Subgenomic flaviviral RNAs (sfRNAs) are virus-derived noncoding produced by pathogenic mosquito-borne flaviviruses (MBF) to counteract the host antiviral response. To date, ability of non-pathogenic produce and utilise sfRNAs remains largely unexplored, it is unclear what role XRN1 resistance plays in flavivirus evolution adaptation. Herein production several insect-specific (ISFs) that replicate exclusively mosquitoes shown, secondary structures their complete 3'UTRs determined. The xrRNAs...
Chikungunya virus (CHIKV) belongs to a group of mosquito-borne alphaviruses associated with acute and chronic arthropathy, peripheral limb joints most commonly affected. Using mouse model CHIKV infection arthritic disease, we show that replication the ensuing foot arthropathy were dramatically reduced when mice housed at 30°C, rather than conventional 22°C. The effect was not detectable fever, but dependent on type I interferon responses. Bioinformatics analyses RNA-Seq data after injection...
Abstract N-linked glycosylation of flavivirus envelope proteins is widely viewed as being required for optimal folding, processing and/or transit proteins, and the assembling virons, through endoplasmic reticulum (ER) Golgi. Zika virus (ZIKV) has a single glycan located adjacent to fusion loop. Herein we show that independent serial passage ZIKVNatal in Rag1-/- mice 223 or 386 days, generated two unique glycan-deficient mutants, ZIKV-V153D ZIKV-N154D, respectively. Surprisingly, these...
Extracellular vesicles (EVs) are small membrane secreted by the cells that mediate intercellular transfer of molecules and contribute to transduction various signals. Viral infection action pro-inflammatory cytokines has been shown alter molecular composition EV content. Transfer antiviral proteins EVs is thought development inflammation state. Altered incorporation selected host RNAs into in response also demonstrated for several viruses, but not WNV. Considering medical significance...
Binjari virus (BinJV) is a lineage II or dual-host affiliated insect-specific flavivirus previously demonstrated as replication-deficient in vertebrate cells. Previous studies have shown that BinJV tolerant to exchanging its structural proteins (prM-E) with pathogenic flaviviruses, making it safe backbone for vaccines. Here, we report generation by circular polymerase extension reaction of expressing zsGreen mCherry fluorescent protein. Recovered reporter viruses grew high titres (107−8...
Neural epidermal growth factor-like like 2 (NELL2) is a cytoplasmic and secreted glycosylated protein with six domains. In animal models, NELL2 predominantly expressed in neural tissues where it regulates neuronal differentiation, polarization, axon guidance, but little known about the role of human brain development. this study, we show that rostral stem cells (rNSC) derived from human-induced pluripotent cell (hiPSC) exhibit particularly strong expression enriched at apical side rosettes...
Zika virus (ZIKV) has a unique ability among flaviviruses to cross the placental barrier and infect fetal brain causing severe abnormalities of neurodevelopment known collectively as congenital syndrome. In our recent study, we demonstrated that viral noncoding RNA (subgenomic flaviviral RNA, sfRNA) induces apoptosis neural progenitors is required for ZIKV pathogenesis in developing brain. Herein, expanded on initial findings identified biological processes signaling pathways affected by...
Getah virus (GETV) is a mosquito-transmitted alphavirus primarily associated with disease in horses and pigs Asia. GETV was also reported to have been isolated from mosquitoes Australia 1961; however, retrieval sequencing of the original isolates (N544 N554), illustrated that these viruses were virtually identical 1955 GETVMM2021 isolate Malaysia. K-mer mining >40,000 terabases sequence data Sequence Read Archive followed by BLASTn confirmation identified multiple sequences biosamples Asia...
Poxvirus systems have been extensively used as vaccine vectors. Herein a RNA-Seq analysis of intramuscular injection sites provided detailed insights into host innate immune responses, well expression vector and recombinant immunogen genes, after vaccination with new multiplication defective, vaccinia-based vector, Sementis Copenhagen Vector. Chikungunya Zika virus mRNA protein was associated necrosing skeletal muscle cells surrounded by mixed cellular infiltrates. The multiple adjuvant...
Flavivirids are small, enveloped, positive-sense RNA viruses from the family Flaviviridae with genomes of ~9–13 kb. Metatranscriptomic analyses metazoan organisms have revealed a diversity flavivirus-like or flavivirid viral sequences in fish and marine invertebrate groups. However, no virus has been identified amphibians. To remedy this, we investigated virome European common frog ( Rana temporaria ) UK, utilizing high-throughput sequencing at six catch locations. De novo assembly...
West Nile virus (WNV) is a neurotropic flavivirus that can cause encephalitis in mammalian and avian hosts. In America, the virulent WNV strain (NY99) causing yearly outbreaks of humans horses, while Australia less Kunjin has not been associated with significant disease until recent 2011 large outbreak horses (but humans) caused by NSW2011 strain. Using chimeric viruses between NY99 strains we previously identified role for non-structural proteins especially NS3 protein, enhanced replication...
ABSTRACT Why individuals with Down Syndrome (DS, trisomy 21) are particularly susceptible to SARS CoV-2 induced neuropathology remains largely unclear. Since the choroid plexus (CP) performs important barrier and immune-interface functions, secretes cerebrospinal fluid strongly expresses ACE2 receptor chromosome 21 encoded TMPRSS2 protease, we hypothesized that CP could play a role in establishing SARS-CoV-2 infection brain. To investigate of central nervous system DS, established new type...
During 2015-2016, outbreaks of Zika virus (ZIKV) occurred in Southeast Asia and the Americas. Most ZIKV infections humans are asymptomatic, while clinical manifestation is usually a self-limiting febrile disease with maculopapular rash. However, capable inducing range severe neurological complications collectively described as congenital syndrome (CZS). Notably, scale magnitude were significantly smaller compared to those Sequence comparison between epidemic-associated strains from Americas...
Tick-borne orthoflaviviruses (TBFs) are classified into three conventional groups based on genetics and ecology: mammalian, seabird probable-TBF group. Recently, a fourth basal group has been identified in Rhipicephalus ticks from Africa: Mpulungu flavivirus (MPFV) Zambia Ngoye virus (NGOV) Senegal. Despite attempts, isolating these viruses vertebrate invertebrate cell lines or intracerebral injection of newborn mice with virus-containing homogenates remained unsuccessful. In this study, we...