A5005824495- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Neuroinflammation and Neurodegeneration Mechanisms
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- HIV Research and Treatment
- Animal Virus Infections Studies
- Extracellular vesicles in disease
- Long-Term Effects of COVID-19
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Mine drainage and remediation techniques
- COVID-19 Clinical Research Studies
- COVID-19 and Mental Health
- SARS-CoV-2 detection and testing
- Herpesvirus Infections and Treatments
- interferon and immune responses
- Invertebrate Immune Response Mechanisms
- Phosphorus and nutrient management
- Bauxite Residue and Utilization
- Virus-based gene therapy research
- Hepatitis Viruses Studies and Epidemiology
- Inflammasome and immune disorders
The University of Queensland
2017-2024
Commonwealth Scientific and Industrial Research Organisation
2023
CSIRO Health and Biosecurity
2023
Abstract The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite large size of viral RNA genome (~30 kb), infectious full-length cDNA readily assembled in vitro a circular polymerase extension reaction (CPER) methodology without need for technically demanding intermediate steps. Overlapping fragments are generated from and together with linker fragment containing CMV promoter into single reaction. Transfection...
Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson's disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote activation. Using transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) as COVID-19 pre-clinical model,...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 and is capable human-to-human transmission rapid global spread. The emergence spread SARS-CoV-2 encouraged establishment a rapid, sensitive, reliable viral detection quantification methodology. Here, we present an alternative assay, termed immuno-plaque assay (iPA), which utilizes combination plaque immunofluorescence techniques. We have extensively optimized...
Abstract Flaviviruses, including Zika virus (ZIKV), utilise host mRNA degradation machinery to produce subgenomic flaviviral RNA (sfRNA). In mammalian hosts, this noncoding facilitates replication and pathogenesis of flaviviruses by inhibiting IFN-signalling, whereas the function sfRNA in mosquitoes remains largely elusive. Herein, we conduct a series vitro vivo experiments define role ZIKV infected Aedes aegypti employing viruses deficient production sfRNA. We show that sfRNA-deficient have...
Zika virus (ZIKV) has recently emerged and is the etiological agent of congenital syndrome (CZS), a spectrum abnormalities arising from neural tissue infections in utero. Herein, we describe de novo generation new ZIKV isolate, ZIKVNatal, using modified circular polymerase extension reaction protocol sequence data obtained ZIKV-infected fetus with microcephaly. ZIKVNatal thus no laboratory passage history unequivocally associated CZS. could be used to establish fetal brain infection model...
All flaviviruses, including Zika virus, produce noncoding subgenomic flaviviral RNA (sfRNA), which plays an important role in viral pathogenesis. However, the exact mechanism of how sfRNA enables evasion antiviral response is not well defined. Here, we show that required for transplacental virus dissemination pregnant mice and subsequent fetal brain infection. We also promotes apoptosis neural progenitor cells human organoids, leading to their disintegration. In infected placental cells,...
The Rabbit haemorrhagic disease virus (RHDV) was discovered 40 years ago. This highly pathogenic threatens the integrity of ecosystems in European rabbits' native range, while Australia, it is used as a biocontrol tool to manage overabundant populations feral rabbits (Oryctolagus cuniculus). Little known about life cycle this due absence reliable cell culture system. In 2023, we developed rabbit liver-derived organoid system that supports RHDV replication but unable sustain serial passaging...
Globally, mineral processing activities produce an estimated 680 GL/yr of alkaline wastewater. Neutralizing pH and removing dissolved elements are the main goals wastewater treatment prior to discharge. Here, we present first study explicitly evaluate role microbial communities in driving neutralization element removal wastewaters by fermentation organic carbon, using bauxite residue leachate as a model system, effects carbon complexity inoculum addition rates on performance these systems at...
Rocio virus (ROCV) is a highly neuropathogenic mosquito-transmitted flavivirus responsible for an unprecedented outbreak of human encephalitis during 1975–1976 in Sao Paulo State, Brazil. Previous studies have shown increased number inflammatory macrophages the central nervous system (CNS) ROCV-infected mice, implying role pathogenesis ROCV. Here, we show that ROCV infection results expression CCL2 blood and infiltration into brain. Moreover, show, using CCR2 knockout essential macrophage...
Australia has multiple lagoviruses with differing pathogenicity. The circulation of these viruses was traditionally determined through opportunistic sampling events. In the lead up to nationwide release RHDVa-K5 (GI.1aP-GI.1a) in 2017, an existing citizen science program, RabbitScan, augmented allow members public submit samples collected from dead leporids for lagovirus testing. This study describes information obtained increased number leporid received between 2015 and 2022 focuses on...
West Nile virus (WNV) is a neurotropic flavivirus that can cause encephalitis in mammalian and avian hosts. In America, the virulent WNV strain (NY99) causing yearly outbreaks of humans horses, while Australia less Kunjin has not been associated with significant disease until recent 2011 large outbreak horses (but humans) caused by NSW2011 strain. Using chimeric viruses between NY99 strains we previously identified role for non-structural proteins especially NS3 protein, enhanced replication...
The recent emergence of Zika virus (ZIKV) in Brazil was associated with an increased number fetal brain infections that resulted a spectrum congenital neurological complications known as syndrome (CZS). Herein, we generated de novo from sequence data early Asian lineage ZIKV isolate (ZIKV-MY; Malaysia, 1966) not microcephaly and compared the vitro replication kinetics infection interferon α/β receptor 1 knockout (IFNAR1−/−) dams this Brazilian (ZIKV-Natal; Natal, 2015) unequivocally...
The genus Lagovirus of the family Caliciviridae contains some most virulent vertebrate viruses known. Lagoviruses infect leporids, such as rabbits, hares and cottontails. Highly pathogenic Rabbit haemorrhagic disease virus 1 (RHDV1) cause a fulminant hepatitis that typically leads to disseminated intravascular coagulation within 24–72 h infection, killing over 95 % susceptible animals. Research into pathophysiological mechanisms are responsible for this extreme phenotype has been hampered by...
ABSTRACT Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson’s disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote utilising model human monocyte-derived microglia. We identified isolates bind and enter microglia, triggering in...
The identification of four potential nonstructural 5 (NS5) residues-K28, K45, V335, and S749-that share the same amino acid preference in STAT2-interacting flaviviruses [Dengue virus (DENV) Zika (ZIKV)], but not STAT2-non-interacting [West Nile (WNV) and/or Yellow fever (YFV)] from an alignment multiple flavivirus NS5 sequences, implied a possible association with efficiency ZIKV to antagonize human signal transducer activator transcription factor 2 (STAT2). Through site-directed mutagenesis...
Abstract Mosquito-transmitted flavivirus Rocio (ROCV) was responsible for an outbreak of encephalitis in the Ribeira Valley, located south coast Sao Paulo State, Brazil, 1975–1976. ROCV also causes fatal adult mice. Seroprevalence studies humans, horses and water buffaloes different regions Brazil have suggested that is still circulating country, indicating risk re-emergence this virus. West Nile virus (WNV) a mosquito-transmitted encephalitic flavivirus, however, WNV strains Australia not...
During 2015-2016, outbreaks of Zika virus (ZIKV) occurred in Southeast Asia and the Americas. Most ZIKV infections humans are asymptomatic, while clinical manifestation is usually a self-limiting febrile disease with maculopapular rash. However, capable inducing range severe neurological complications collectively described as congenital syndrome (CZS). Notably, scale magnitude were significantly smaller compared to those Sequence comparison between epidemic-associated strains from Americas...
Similar to other flaviviruses, Zika virus (ZIKV) produces abundant subgenomic flavivirus RNA (sfRNA) derived from the 3' untranslated region. The molecular mechanisms that determine functions of sfRNA are currently not completely understood. Here, we created ZIKV mutants deficient in production and employed them investigate role this interactions with mammalian insect hosts. We found mosquitoes, facilitates replication is required for dissemination into saliva transmission. was have no...
Abstract Zika virus (ZIKV) is a re-emerging pathogenic flavivirus, which causes microcephaly in infants and poses continuing threat to public health. ZIKV, like all other flaviviruses, produces highly abundant noncoding RNA known as subgenomic flaviviral (sfRNA). Herein we utilized wild-type mutant ZIKV defective production of sfRNA elucidate for the first time how affects aspects pathogenesis. We found that mouse pregnancy model infection required trans-placental dissemination subsequent...