A5026494955- Sleep and Wakefulness Research
- Stress Responses and Cortisol
- Sleep and related disorders
- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Tryptophan and brain disorders
- Pain Mechanisms and Treatments
- Neurotransmitter Receptor Influence on Behavior
- Animal Nutrition and Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Regulation of Appetite and Obesity
- Pain Management and Placebo Effect
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Rabbits: Nutrition, Reproduction, Health
- Birth, Development, and Health
- Circadian rhythm and melatonin
- Reproductive System and Pregnancy
- Neuropeptides and Animal Physiology
Beth Israel Deaconess Medical Center
2022-2024
Harvard University
2022-2024
Hadassah Medical Center
2024
Universidade de São Paulo
2017-2023
University of Missouri
2019-2022
Missouri College
2021-2022
Universidade de Ribeirão Preto
2017-2019
University of Toronto
2018
Rocio virus (ROCV) is a highly neuropathogenic mosquito-transmitted flavivirus responsible for an unprecedented outbreak of human encephalitis during 1975–1976 in Sao Paulo State, Brazil. Previous studies have shown increased number inflammatory macrophages the central nervous system (CNS) ROCV-infected mice, implying role pathogenesis ROCV. Here, we show that ROCV infection results expression CCL2 blood and infiltration into brain. Moreover, show, using CCR2 knockout essential macrophage...
Chronic pain increases the risk of developing anxiety, with limbic areas being likely neurological substrates. Despite high clinical relevance, little is known about precise behavioral, hormonal, and brain neuroplastic correlates anxiety in context persistent pain. Previous studies have shown that decreased nociceptive thresholds chronic models are paralleled by anxiety-like behavior rats, but there conflicting ideas regarding its effects on stress response circulating corticosterone levels....
Maternal stress has a profound impact on the long-term behavioral phenotype of offspring, including responses to stressful and social situations. In this study, we examined effects maternal exposure predator odor, an ethologically relevant psychogenic stressor, stress-induced behaviors in both semi-naturalistic laboratory-based Adult C57BL/6 mice offspring dams exposed odor during last half pregnancy showed increased anti-predatory behavior, more cautious foraging behavior and, elevated plus...
Orexin neurons are sensitive to CO
Depending on its duration and severity, stress may contribute to neuropsychiatric diseases such as depression anxiety. Studies have shown that impacts the hypothalamic–pituitary–adrenal (HPA) axis, but downstream molecular, behavioral, nociceptive effects remain unclear. We hypothesized a 2-hour single exposure acute restraint (ARS) activates HPA axis changes DNA methylation, molecular mechanism involved in machinery of regulation. further ARS induces anxiety-like risk assessment behavior...
Studies have used paradigms based on animal models to understand human emotional behavior because they appear be correlated with fear- and anxiety-related defensive patterns in non-human mammals. In this context, tonic immobility (TI) is an innate response associated extreme threat situations, such as predator attack. Some reports demonstrated the involvement of corticotropin-releasing factor (CRF) regulation endocrine system, behaviors behavioral responses stress. Particularly, a previous...
Orexin neurons are active in wakefulness and mostly silent sleep. In adult rats humans, orexin facilitates the hypercapnic ventilatory response but has little effect on resting ventilation. The influence of breathing early postnatal period, across states vigilance, have not been investigated. This is relevant as system may be impaired Sudden Infant Death Syndrome (SIDS) cases. We addressed three hypotheses: 1) provides a drive to breathe infancy; 2) depends stage development; 3) greater...
Abstract Introduction Many neurons in the external lateral PB express calcitonin gene-related peptide [CGRP; PBelCGRP], and also receive spinal afferents carrying pain signals that are relayed to forebrain arousal promoting areas. Thus, PBelCGRP form critical relay node transmitting induce cortical arousals. We tested this with genetic deletion or acute optogenetic inhibition both inflammatory opto-pain models, found blocking prevents pain-induced Our current focus is on understanding which...
Abstract Pain therapies that alleviate both pain and sleep disturbances may be the most effective for relief, as chronic loss render opioidergic system, targeted by opioids, less sensitive analgesia. Therefore, we first studied link between activation of nociceptors in two acute models. Activation inflammatory (AIP) opto-pain models led to loss, decreased spindle density, increased fragmentation lasted 3 6 hours. This relationship is facilitated transmission nociceptive signals through...
Previously we showed that a loss of central nervous system (CNS) 5-hydroxytryptamine (5-HT) (tryptophan hydroxylase 2 knockout; TPH2-/-) leads to hypertension in male rats during wakefulness and REM sleep. Here, tested the hypotheses is also revealed female TPH2-/- when sex hormones are controlled, especially high arterial blood pressure (ABP) due increased sympathetic vascular tone. The ABP females was measured specifically proestrus or estrus again following ovariectomy. males before after...
Predation is a key organizing force in ecosystems. The threat of predation may act to programme the endocrine hypothalamic–pituitary–adrenal axis during development prepare offspring for environment they are likely encounter. Such effects typically investigated through measurement corticosteroids (Cort). Corticosteroid-binding globulin (CBG) plays role regulating bioavailability Cort, with only free unbound Cort being biologically active. We prenatal predator odour exposure (POE) mice on CBG...
Orexinergic neurons in the lateral and perifornical hypothalamus project widely to respiratory regions of brainstem. Also, orexin are active wakefulness silent sleep. In adult animals, chronic deficiency reduces response CO2, but has no apparent effect on resting ventilation. The acute effects breathing have not been described or infant animals. This topic is important because narcoleptic patients a higher incidence sleep apnea, there pathological evidence orexinergic dysfunction Sudden...
Orexin neurons project to nuclei participating in the hypoxic ventilatory response (HVR), including nucleus tractus solitarius (nTS) and paraventricular of hypothalamus (PVN), that also express orexin receptors (OxRs). Many nTS-projecting PVN are activated by acute hypoxia (Hx) immunoreactive (IR) for corticotropin-releasing hormone (CRH). active dark (active) phase silent light (inactive) phase. contributes HVR males, especially The possibility facilitate through pathways involve and/or nTS...
Orexin is a hypothalamic neuropeptide involved in variety of functions including arousal, body temperature regulation, and feeding. neurons are active wakefulness (or the dark phase rats) mostly silent sleep (light phase), implying effects orexin greater and/or phase. Recent evidence implicates control cardiorespiratory function, respiratory response to CO2 blood pressure regulation. Whether influences normal breathing state-dependent manner unresolved. In addition, although role hypercapnic...