A5080117463- Birth, Development, and Health
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Epigenetics and DNA Methylation
- Infant Nutrition and Health
- Neonatal Respiratory Health Research
- Immunotherapy and Immune Responses
- Early Childhood Education and Development
- Metabolism and Genetic Disorders
- Genetics and Neurodevelopmental Disorders
- Immune Cell Function and Interaction
- Climate Change and Health Impacts
- Clinical Nutrition and Gastroenterology
- Immune Response and Inflammation
- Obesity, Physical Activity, Diet
- Amyotrophic Lateral Sclerosis Research
- African history and culture studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Child Nutrition and Water Access
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Identity, Memory, and Therapy
- Circadian rhythm and melatonin
- Breastfeeding Practices and Influences
University of Toronto
2014-2024
The Scarborough Hospital
2012-2022
University of Liverpool
1991-2014
Liverpool Women's Hospital
2013-2014
Liverpool Womens NHS Foundation Trust
2014
McGill University
2007-2011
Douglas Mental Health University Institute
2007-2011
Duke University
2011
McGill University Health Centre
2008
Université de Montréal
2007
A costimulatory signal through B7 to its counter-receptor CD28 on T cells enhances cell activation. We have generated recombinant retroviruses containing cDNA for murine and transduced a panel of tumor lines with varying immunogenicity study the effect costimulation antitumor immunity. In contrast progressive outgrowth all wild-type (B7-) tumors in unimmunized syngeneic mice, four immunogenic tumors, lymphoma RMA, EL4, mastocytoma P815, melanoma E6B2, regressed completely when gene....
Background Maternal care is associated with long-term effects on behavior and epigenetic programming of the NR3C1 (GLUCOCORTICOID RECEPTOR) gene in hippocampus both rats humans. In rat, these are reversed by cross-fostering, demonstrating that they defined rather than genetic processes. However, changes at a single promoter unlikely to account for range outcomes persistent change expression hundreds additional genes adult response differences maternal care. Methodology/Principal Findings We...
Background Alterations in gene expression the suicide brain have been reported and for several genes DNA methylation as an epigenetic regulator is thought to play a role. rRNA genes, that encode ribosomal RNA, are backbone of protein synthesis machinery levels promoter determine transcription.Methodology/Principal FindingsWe test here by sodium bisulfite mapping quantitative real-time PCR hypothesis differences critical loci involved pathophysiology suicide. Suicide subjects this study were...
Early life experience is associated with long-term effects on behavior and epigenetic programming of the NR3C1 ( GLUCOCORTICOID RECEPTOR ) gene in hippocampus both rats humans. However, it unlikely that such completely capture evolutionarily conserved mechanisms early adaptation to environment. Here we present DNA methylation profiles spanning 6.5 million base pairs centered at humans who experienced abuse as children nonabused controls. We compare these corresponding received differential...
Summary Biomedical researchers have long appreciated that maternal stressors can induce preparative and adaptive programming in offspring via exposure to Glucocorticoids ( GC s). However, few ecologists are aware of the capacity for translate ecological environmental into programmed responses free‐living systems. We review a growing body experimental work indicating circulating s link with stress axis. Throughout, we emphasise natural human‐induced play fundamental role individuals,...
BACKGROUND: Early postnatal head growth failure is well recognized in very preterm infants (VPIs). This coincides with the characteristic nutritional deficits that occur these parenteral nutrition (PN) dependent first month of life. Head circumference (HC) correlated brain volume and later neurodevelopmental outcome. We hypothesized a Standardized, Concentrated With Added Macronutrients Parenteral (SCAMP) regimen would improve early growth. The aim was to compare change HC (ΔHC) SD score...
Perinatal stress mediated through the mother can lead to long-term alterations in stress-related phenotypes offspring. The capacity for adaptation adversity early life depends part on history of animal. This study was designed examine behavioral and neural response adult offspring prenatal exposure predator odor: an ethologically-relevant psychological stressor. Pregnant mice were exposed daily odors or distilled water control over second half pregnancy. Predator odor a transient decrease...
Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) challenging due to the lack standardized methods are currently being used for milk pre-processing, storage, and exosome isolation. In this study, we tested: 1) three pre-processing remove cream, fat, cellular debris, casein proteins from bovine determine whether whole prior long-term storage improves MDE...
Aminoglycosides are commonly prescribed antibiotics used for the treatment of neonatal sepsis. The MT-RNR1 m.1555A>G variant predisposes to profound aminoglycoside-induced ototoxicity (AIO). Current genotyping approaches take several days, which is unfeasible in acute settings. To develop a rapid point-of-care test (POCT) before implementation this technology setting guide antibiotic prescribing and avoid AIO. This pragmatic prospective trial recruited neonates admitted 2 large intensive...
Interactions between the costimulatory molecule B7 on APC and its counter-receptor CD28 T lymphocytes play a key role in induction of cell-mediated immune responses. We studied costimulation tumor-reactive cells by destruction K1735-M2 mouse melanoma into which gene encoding human melanoma-associated Ag, p97, had been transfected. Previous work has demonstrated that p97 transfectant cl62 is immunogenic but still grows progressively immunocompetent C3H/HeN mice adoptive transfer p97-specific...
Rat dams show natural variations in maternal care, licking and grooming (LG), that are associated with distinct behavioral neural phenotypes offspring. However, there has been limited research on the effects of differences LG received by female pups care within litter. Here, we investigated measuring active after pup retrieval We then examined locomotor activity, open field exploration, restraint stress reactivity adult also expression mineralocorticoid receptor (MR) glucocorticoid (GR) DNA...
Cytolytic T cells play a major role in controlling herpes simplex virus type 2 (HSV-2) infections humans. In an effort to more thoroughly evaluate the response HSV-2 directly, ex vivo, we developed enzyme-linked immunospot (ELISPOT) assay that utilized pools of overlapping synthetic peptides presented by autologous dendritic purified CD8(+) cells. Donor rates individual open reading frames (ORFs) ranged from fewer than 5% responding as many 70% responding, with greatest frequency responses...