A5032528144- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Epigenetics and DNA Methylation
- Bat Biology and Ecology Studies
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Birth, Development, and Health
- GDF15 and Related Biomarkers
- Infant Nutrition and Health
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Neuroendocrine regulation and behavior
- Child Nutrition and Water Access
- RNA Interference and Gene Delivery
- Heat shock proteins research
- Breastfeeding Practices and Influences
- Stress Responses and Cortisol
- Circular RNAs in diseases
- Sirtuins and Resveratrol in Medicine
- thermodynamics and calorimetric analyses
- Erythrocyte Function and Pathophysiology
- Effects of Environmental Stressors on Livestock
- Environmental DNA in Biodiversity Studies
- Protist diversity and phylogeny
University of Winnipeg
2021-2025
University of Toronto
2019-2022
Carleton University
2016-2021
University of Ottawa
2017
Milk is a highly complex, heterogeneous biological fluid that contains non-nutritive, bioactive extracellular vesicles called exosomes. Characterization of milk-derived exosomes (MDEs) challenging due to the lack standardized methods are currently being used for milk pre-processing, storage, and exosome isolation. In this study, we tested: 1) three pre-processing remove cream, fat, cellular debris, casein proteins from bovine determine whether whole prior long-term storage improves MDE...
Proteotoxic stress induces microglia polarization and attenuates cytoprotective, pro-survival cellular cascades. Milk-derived extracellular vesicles (MEVs) are lipid-coated nanovesicles that combat pro-inflammation in peripheral cells tissues; however, the cytoprotective potential of MEVs remains unknown brain macrophages. We investigated whether reduce neuroinflammation human by activating heat shock response (HSR). The HSR triggers upregulation molecular chaperones (heat proteins; HSPs) to...
The common wood frog, Rana sylvatica, utilizes freeze tolerance as a means of winter survival. Concealed beneath layer leaf litter and blanketed by snow, these frogs withstand subzero temperatures allowing approximately 65-70% total body water to freeze. Freezing is generally considered be an ischemic event in which the blood oxygen supply impeded may lead low levels ATP production exposure oxidative stress. Therefore, it important selectively upregulate cytoprotective mechanisms such heat...
Abstract Maternal consumption of a high-fat diet (mHFD) during perinatal life (the collective prenatal and postnatal periods) influences neonatal development, initiates hypothalamic-pituitary-adrenal (HPA) axis activation, impacts the long-term physiological metabolic health offspring. Milk-derived extracellular vesicles (MEVs) are lipid-coated nanovesicles found in mammalian milk that survive intestinal degradation cross complex biological barriers, including blood-brain barrier. MEVs have...
Exposure to a maternal high fat diet (HFD) during perinatal (prenatal and postnatal combined) life increases offspring's risk of developing metabolic diseases (obesity, type II diabetes hypertension), impairs immunity, behavior, neurodevelopment. Exclusive breast/chest milk feeding is potential solution reduce the negative developmental effects HFD, mainly chronic systemic pro-inflammation. This study focuses on analyzing anti-inflammatory group biological nanovesicles found in human milk,...
African clawed frogs (Xenopus laevis) endure bouts of severe drought in their natural habitats and survive the loss approximately 30% total body water due to dehydration. To investigate molecular mechanisms employed by X. laevis during periods dehydration, heat shock protein response, a vital component cytoprotective stress was characterized. Using western immunoblotting multiplex technology, levels HSP27, HSP40, HSP60, HSP70, HSC70, HSP90 were quantified liver, skeletal muscle, kidney,...
Abstract Background Maternal obesity as a result of high levels saturated fat (HFD) consumption leads to significant negative health outcomes in both mother and exposed offspring. Offspring maternal HFD show sex-specific alterations metabolic, behavioral, endocrine function, well increased basal neuroinflammation that persists into adulthood. There is evidence psychosocial stress or exogenous administration corticosterone (CORT) potentiate inflammatory gene expression; however, the response...
Leptospirosis is a disease of epidemic proportions in Sri Lanka. There paucity data on the determinants mortality and impact therapy patients with leptospirosis admitted to critical care settings endemic territories.This retrospective cross-sectional study was performed serologically confirmed intensive unit General Hospital, Kalutara from January 2011 April 2014. Associations between socio-epidemiological, clinical laboratory parameters patient were examined.Forty-five included. The mean...
As a result of suppressed recombination, heterogametic sex chromosomes (either Y or W) are usually assumed to gradually shorten over evolutionary time as way remove accumulated mutations. However, recombination removes the most obvious mechanism for excising portions chromosomes. We examined ratios W/Z chromosome size across 224 bird species from 146 genera. Much data were obtained previous study (Rutkowska et al. 2012. Biology Letters8: 636–638), who, similar ourselves, found no gradual...
Abstract Adverse maternal diets high in saturated fats are associated with impaired neurodevelopment and epigenetic modifications offspring. Maternal milk, the primary source of early life nutrition mammals, contains lactation-specific microRNAs (miRNAs). Lactation-specific miRNAs have been found various offspring tissues life, including brain. We examined effects fat diet (mHFD) on that inhibit DNA methyltransferases (DNMTs), enzymes catalyze methylation modifications, amygdala female...