A5010253923- Physiological and biochemical adaptations
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- RNA modifications and cancer
- Indigenous Studies and Ecology
- Cancer, Hypoxia, and Metabolism
- Genomics and Chromatin Dynamics
- Pancreatic function and diabetes
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Plant Molecular Biology Research
- Metabolism, Diabetes, and Cancer
- Bat Biology and Ecology Studies
- Metabolomics and Mass Spectrometry Studies
- Extracellular vesicles in disease
- Genetics and Neurodevelopmental Disorders
- Enzyme function and inhibition
- Social Sciences and Governance
- FOXO transcription factor regulation
- Cell Image Analysis Techniques
- Molecular Biology Techniques and Applications
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
Carleton University
2016-2024
Dalhousie University
2016
The wood frog is one of the few freeze-tolerance vertebrates. This accomplished in part by accumulation cryoprotectant glucose, metabolic rate depression, and stress response activation. These may be achieved mechanisms such as DNA methylation, which typically associated with transcriptional repression. Hyperglycemia also modifications to epigenetic profiles, indicating an additional role that high levels glucose play freeze tolerance. We sought determine whether methylation affected during...
During the hibernation season, thirteen-lined ground squirrel undergoes cyclical torpor and arousal periods. The decrease restoration of metabolic rate oxygen delivery during arousal, respectively, may cause reperfusion-ischemia injury in kidneys. In order to maintain structural integrity kidneys necessary for renal function resumption has developed adaptive methods prevent repair kidney injury. this present study, computational were used clean analyze sequenced RNA samples. Significantly...
Every cell in an individual has largely the same genomic sequence and yet cells different tissues can present widely phenotypes. This variation arises because each expresses a specific subset of instructions. Control over which instructions, or genes, are expressed is controlled by transcriptional regulatory pathways. Each must assimilate huge amount environmental input, thus it no surprise that transcription regulated many intertwining mechanisms. large landscape means there ample...
Abstract Naked mole rats are a long‐lived animal model that age much like humans, but can also withstand oxidative damage, cancer, neurodegenerative diseases, and severe hypoxic conditions, which is of particular interest to this study. The conditions their underground burrows result in competition for oxygen consumption, yet despite deprivation they emerge unscathed. To understand the mechanisms place facilitate neuronal preservation during hypoxia, we investigated protein levels well‐known...
Xenopus laevis tolerate dehydration when their environments evaporate during summer months. Protein phosphorylation has previously shown to regulate important adaptations in X. laevis, including the transition anaerobic metabolism. We therefore performed phosphoproteomic analysis of further elucidate cellular and metabolic responses dehydration. Phosphoproteins were enriched functions pathways related glycolysis/gluconeogenesis, TCA cycle, protein metabolism, among others. The prominence...
Common Western-blot imaging systems have previously been adapted to measure signals from luminescent microplate assays. This can be a cost saving as are common laboratory equipment and could substitute dedicated luminometer if one is not otherwise available. One unrecognized limitation that the captured by cameras in these equal for all wells. Signals dependent on angle of incidence camera, thus location well microplate. Here we show that:•The position significantly affects signal system...
Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While few other organisms, nematodes, have subsequently also been found in this manner, P. DAW1 so far shown highest survival rates. In addition, is also, depending on rate or extent freezing, able undergo cryoprotective dehydration. study, proteome sp explored, highlighting number differentially expressed proteins...