A5055393465- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Circadian rhythm and melatonin
- Sleep and related disorders
- Neuroscience and Neuropharmacology Research
- Obstructive Sleep Apnea Research
- Regulation of Appetite and Obesity
- Pain Mechanisms and Treatments
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Neuropeptides and Animal Physiology
- Drug Solubulity and Delivery Systems
- Chemical synthesis and pharmacological studies
- Lipid Membrane Structure and Behavior
- Herbal Medicine Research Studies
- Helminth infection and control
- Dermatology and Skin Diseases
- Advancements in Transdermal Drug Delivery
- Alcohol Consumption and Health Effects
- Neural dynamics and brain function
- Toxin Mechanisms and Immunotoxins
- Nanoparticle-Based Drug Delivery
- Advanced Drug Delivery Systems
- Botanical Research and Chemistry
- Contact Dermatitis and Allergies
Harvard University
2008-2024
Beth Israel Deaconess Medical Center
2013-2024
Hadassah Medical Center
2021-2024
Chitkara University
2022-2024
Public Works Department Buildings and Roads
2017
Beth Israel Deaconess Hospital
2014
Boston University
2014
Harvard University Press
2009
Hesco (United States)
2008
Veterans of Foreign Wars
2008
The mechanisms of arousal from apneas during sleep in patients suffering obstructive apnea are not well understood. However, we know that respiratory chemosensory pathways converge on the parabrachial nucleus (PB), which sends glutamatergic projections to a variety forebrain structures critical arousal, including basal forebrain, lateral hypothalamus, midline thalamus, and cerebral cortex. We tested role signaling this pathway by developing an animal model for repetitive CO 2 arousals (RCAs)...
The basal forebrain (BF) is known for its role in cortical and behavioral activation, has been postulated to have a compensatory mechanisms after sleep loss. However, specific neuronal phenotypes responsible these roles are unclear. We investigated the effects of ibotenate (IBO) 192IgG-saporin (SAP) lesions caudal BF on spontaneous sleep-waking electroencephalogram (EEG), recovery EEG 6 h deprivation (SD). Relative artificial CSF (ACSF) controls, IBO injections decreased parvalbumin...
ABSTRACT In patients with obstructive sleep apnea, airway obstruction during produces hypercapnia, which in turn activates respiratory muscles that pump air into the lungs (e.g., diaphragm) and dilate stabilize upper genioglossus). We hypothesized these responses are facilitated by glutamatergic neurons parabrachial complex (PB) respond to hypercapnia project premotor motor innervate diaphragm genioglossus muscles. To test this hypothesis, we combined c‐Fos immunohistochemistry situ...
Abstract During obstructive sleep apnea, elevation of CO 2 during apneas contributes to awakening and restoring airway patency. We previously found that glutamatergic neurons in the external lateral parabrachial nucleus (PBel) containing calcitonin gene related peptide (PBel CGRP neurons) are critical for causing arousal hypercapnia. However, others genetic deletion serotonin (5HT) brainstem also prevented from To examine interactions between two systems, we showed dorsal raphe (DR) 5HT...
Spontaneous pain, a major complaint of patients with neuropathic has eluded study because there is no reliable marker in either preclinical models or clinical studies. Here, we performed comprehensive electroencephalogram/electromyogram analysis sleep several mouse chronic pain: (spared nerve injury and constriction injury), inflammatory (Freund’s complete adjuvant carrageenan, plantar incision) chemical pain (capsaicin). We find that peripheral axonal drives fragmentation by increasing...
Ten years ago the sleep disorder narcolepsy was linked to neuropeptide hypocretin (HCRT), also known as orexin. This is characterized by excessive day time sleepiness, inappropriate triggering of rapid-eye movement (REM) and cataplexy, which a sudden loss muscle tone during waking. It still not how HCRT regulates REM or since neurons are localized only in lateral hypothalamus while atonia generated from brainstem. To identify potential neuronal circuit, neurotoxin hypocretin-2-saporin...
Gene transfer has proven to be an effective neurobiological tool in a number of neurodegenerative diseases, but it is not known if can correct sleep disorder. Narcolepsy disorder linked the loss neurons containing neuropeptide orexin, also as hypocretin. Here, replication-defective herpes simplex virus-1 amplicon-based vector was constructed gene for mouse prepro-orexin into mice with genetic deletion orexin gene. After vitro tests confirmed successful cells, delivered lateral hypothalamus...
Abstract About half of the neurons in parabrachial nucleus (PB) that are activated by CO 2 located external lateral (el) subnucleus, express calcitonin gene-related peptide (CGRP), and cause forebrain arousal. We report here, male mice, most remaining -responsive adjacent central (PBcl) Kölliker-Fuse (KF) PB subnuclei transcription factor FoxP2 many these project to respiratory sites medulla. PBcl show increased intracellular calcium during wakefulness REM sleep response elevated NREM sleep....
Abstract GABA in locus coeruleus modulates REM sleep. Apart from the presence of interneurons, also receives GABAergic inputs prepositus hypoglossi medulla, where REM‐ON‐like neurons have been reported. Therefore, it was hypothesized that projections to may modulate The experiments were conducted on chronic rats prepared for recording EEG, EOG, and EMG freely moving conditions. Bipolar stimulating electrodes implanted bilaterally, while chemitrodes bilaterally coeruleus. stimulated (3 Hz,...
Narcolepsy is a human sleep disorder resulting from the loss of neurons containing neuropeptide orexin, also known as hypocretin. Cataplexy, which sudden muscle tone during waking, an important diagnostic symptom narcolepsy. In humans and canines with narcolepsy, cataplexy considered to be separate distinct behavioral state. However, in mouse model disease this issue not resolved. The present study monitored activity forty four rostral pons hypocretin knock-out mice. Majority were active...