A5049486224- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Bacteriophages and microbial interactions
- Long-Term Effects of COVID-19
- Telomeres, Telomerase, and Senescence
- Advanced Vision and Imaging
- Circadian rhythm and melatonin
- Bacterial Infections and Vaccines
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- vaccines and immunoinformatics approaches
- Time Series Analysis and Forecasting
- Inflammasome and immune disorders
- Advanced Fluorescence Microscopy Techniques
- Optical Polarization and Ellipsometry
- Fungal Plant Pathogen Control
- Estrogen and related hormone effects
- Gestational Trophoblastic Disease Studies
- Advanced Optical Imaging Technologies
- Infrared Target Detection Methodologies
The University of Queensland
2021-2024
University of California, Davis
2024
Stanford University
2023
The University of Melbourne
2023
University of California, San Francisco
2020
Duke-NUS Medical School
2016-2017
Kyoto University
2007
University of York
2003-2005
Heriot-Watt University
1999
Abstract The current COVID-19 pandemic is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We demonstrate that despite large size of viral RNA genome (~30 kb), infectious full-length cDNA readily assembled in vitro a circular polymerase extension reaction (CPER) methodology without need for technically demanding intermediate steps. Overlapping fragments are generated from and together with linker fragment containing CMV promoter into single reaction. Transfection...
Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson's disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote activation. Using transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) as COVID-19 pre-clinical model,...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causative agent of coronavirus disease 2019 and is capable human-to-human transmission rapid global spread. The emergence spread SARS-CoV-2 encouraged establishment a rapid, sensitive, reliable viral detection quantification methodology. Here, we present an alternative assay, termed immuno-plaque assay (iPA), which utilizes combination plaque immunofluorescence techniques. We have extensively optimized...
Aging is a major risk factor for neurodegenerative diseases, and coronavirus disease 2019 (COVID-19) linked to severe neurological manifestations. Senescent cells contribute brain aging, but the impact of virus-induced senescence on neuropathologies unknown. Here we show that senescent accumulate in aged human organoids senolytics reduce age-related inflammation rejuvenate transcriptomic aging clocks. In postmortem brains patients with COVID-19 observed increased cell accumulation compared...
A recent study proposed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hijacks the LINE-1 (L1) retrotransposition machinery to integrate into DNA of infected cells. If confirmed, this finding could have significant clinical implications. Here, we apply deep (>50×) long-read Oxford Nanopore Technologies (ONT) sequencing HEK293T cells with SARS-CoV-2 and do not find virus integrated genome. By examining ONT data from separate cultivars, completely resolve 78 L1 insertions...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 160 million people and resulted in 3.3 deaths, despite the availability of multiple vaccines, world still faces many challenges with their rollout. Here, we use high-density microarray patch (HD-MAP) to deliver a SARS-CoV-2 spike subunit vaccine directly skin. We show that is thermostable on patches, delivery enhancing both cellular antibody immune responses. Elicited antibodies potently neutralize clinically...
Abstract In August 2022, a novel henipavirus (HNV) named Langya virus (LayV) was isolated from patients with severe pneumonic disease in China. This is closely related to Mòjiāng (MojV), and both are divergent the bat-borne HNV members, Nipah (NiV) Hendra (HeV) viruses. The spillover of LayV first instance zoonosis humans outside NiV HeV, highlighting continuing threat this genus poses human health. work, we determine prefusion structures MojV F proteins via cryogenic electron microscopy...
Cancers and neurological disorders are two major types of diseases in humans. We developed the concept called “Aberrant Cell Cycle Disease (ACCD)” due to accumulating evidence that shows different share common mechanism aberrant cell cycle re-entry. The re-entry is manifested as kinase/oncoprotein activation tumor suppressor (TS) inactivation, which associated with both growth cancers neuronal death disorders. Therefore, some cancer therapies (e.g., kinase/oncogene inhibition TS elevation)...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) Omicron variant sub-lineages spread rapidly worldwide, mostly due to their immune-evasive properties. This has put a significant part of the population at risk for disease and underscores need effective anti-SARS-CoV-2 agents against emergent strains in vulnerable patients. Camelid nanobodies are attractive therapeutic candidates high stability, ease large-scale production, potential delivery via inhalation. Here, we...
We established animal models of osteoporosis in ovariectomized rats to detect osteoprogerin (Opg)/receptor activator nuclear factor-κB ligand (Rankl) mRNA expression levels the tibias and serum estradiol concentrations at different time points. Sixty Sprague-Dawley female were randomly selected divided into an (OVX) group sham-operated (SHAM) group. In SHAM group, only a small amount abdominal fat tissues was removed from rats. Ten each sacrificed 0, 6, 12 months after establishing (12...
Coronaviruses are a diverse group of viruses that infect mammals and birds. Bats reservoirs for several different coronaviruses in the Alphacoronavirus Betacoronavirus genera. They also appear to be natural reservoir ancestral generated severe acute respiratory syndrome coronavirus Middle East outbreaks. Here, we detected sequences next-generation sequence data created from Eonycteris spelaea faeces urine. We screened by PCR urine samples, faecal samples rectal swabs collected six species...
Bats are unique mammals that reservoirs of high levels virus diversity. Although several these viruses zoonotic, the majority not. Astroviruses, transmitted fecal-orally, commonly detected in a wide diversity bat species, prevalent at rates and not thought to directly infect humans. These features make astroviruses useful examining evolutionary history, epidemiology host, temporal shedding trends. Our study screened for presence bats Singapore, reconstructed phylogenetic relations polymerase...
The ongoing coronavirus disease 2019 (COVID-19) pandemic continues to disrupt essential health services in 90 percent of countries today. spike (S) protein found on the surface causative agent, SARS-CoV-2 virus, has been prime target for current vaccine research since antibodies directed against S were neutralize virus. However, as new variants emerge, mutations within have given rise potential immune evasion response generated by generation vaccines. In this study, a modified, HexaPro...
The SARS-CoV-2 virus has caused a global crisis, resulting in 0.5 billion infections and over 6 million deaths as of March 2022. Fortunately, infection hospitalization rates were curbed due to the rollout DNA mRNA vaccines. However, efficacy these vaccines significantly drops few months post immunization, from 88% down 47% case Pfizer BNT162 vaccine. emergence variant strains, especially delta omicron, have also reduced vaccine efficacy. We propose peptide potential solution address...
Abstract The emergence of SARS-CoV-2 variants alters the efficacy existing immunity towards viral spike protein, whether acquired from infection or vaccination. Mutations that impact N -glycosylation may be particularly important in influencing antigenicity, but their consequences are difficult to predict. Here, we compare glycosylation profiles and antigenicity recombinant ancestral Wu-1 Gamma strain, which has two additional sites due amino acid substitutions N-terminal domain (NTD). We...
Abstract Pirfenidone, an oral anti‐fibrotic and anti‐inflammatory medication used for the treatment of idiopathic pulmonary fibrosis (IPF), has been proposed to treat post‐COVID pneumonia (PF). However, pirfenidone displays side effects which include hepatotoxicity anorexia. Cubane cyclooctatetraene (COT) derivatives were prepared as bioisostere/biomotif replacements phenyl ring explore potential changes in activity. The key intermediate, aminocubane, enabled synthesis both cubane derived...
The use of search engines within the Internet is now ubiquitous. This work examines how Grid technology may affect implementation by focusing on Signal Data Explorer application developed Distributed Aircraft Maintenance Environment (DAME) project. utilizes advanced neural-network-based methods (Advanced Uncertain Reasoning Architecture (AURA) technology) to for matching patterns in time-series vibration data originating from Rolls-Royce aeroengines (jet engines). large volume associated...
Abstract SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 deaths, we still face many challenges the rollout of vaccines. Here, use high-density microarray patch to deliver a spike subunit vaccine directly skin. We show vaccine, dry-coated on is thermostable, delivery via HD-MAP induced greater cellular antibody immune responses, with serum able potently neutralize clinically relevant isolates including those from B.1.1.7 B.1.351 lineages. Finally, single dose...
Abstract Microbial biosynthetic gene clusters are a valuable source of bioactive molecules. However, because they typically represent small fraction genomic material in most metagenomic samples, it remains challenging to deeply sequence them. We present an approach isolate and samples using microfluidic automated plasmid library enrichment. Our provides deep coverage the target cluster, facilitating reassembly. demonstrate by isolating sequencing type I polyketide synthase from Antarctic...
ABSTRACT Coronavirus disease-2019 (COVID-19) is primarily a respiratory disease, however, an increasing number of reports indicate that SARS-CoV-2 infection can also cause severe neurological manifestations, including precipitating cases probable Parkinson’s disease. As microglial NLRP3 inflammasome activation major driver neurodegeneration, here we interrogated whether promote utilising model human monocyte-derived microglia. We identified isolates bind and enter microglia, triggering in...
The emergence of SARS-CoV-2 variants alters the efficacy existing immunity towards viral spike protein, whether acquired from infection or vaccination. Mutations that impact N-glycosylation may be particularly important in influencing antigenicity, but their consequences are difficult to predict. Here, we compare glycosylation profiles and antigenicity recombinant ancestral Wu-1 Gamma strain, which has two additional sites due amino acid substitutions N-terminal domain (NTD). We found a...
This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum. The requires a small amount S-protein/RBD and angiotensin converting enzyme-2 (ACE2). A high-throughput, simple ELISA technique is employed. Plate-coated-RBDs are allowed to interact with the serum, then soluble ACE2 added, followed secondary antibodies substrate. key steps in this include (1) serum heat treatment...