A5101881371- Immunotherapy and Immune Responses
- Cervical Cancer and HPV Research
- T-cell and B-cell Immunology
- Advancements in Transdermal Drug Delivery
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Dermatology and Skin Diseases
- Herpesvirus Infections and Treatments
- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- Neurobiology and Insect Physiology Research
- Intramuscular injections and effects
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Transgenic Plants and Applications
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Toxin Mechanisms and Immunotoxins
- Fatty Acid Research and Health
- T-cell and Retrovirus Studies
- Virology and Viral Diseases
- Advanced Drug Delivery Systems
The University of Queensland
2012-2025
Vaxxas (Australia)
2013-2025
Optimum Therapeutics (United States)
2025
Translational Research Institute
2018-2022
The Royal Free Hospital
2012
University College London
2002-2012
Canberra Hospital
2011
Australian National University
2011
Princess Alexandra Hospital
1998-2009
Pontificia Universidad Católica de Valparaíso
2009
We have identified a major T-cell epitope, amino acids 48-54 (DRAHYNI, in one-letter code) the E7 open reading frame protein of human papillomavirus (HPV) type 16. Lymph node cells from mice immunized with synthetic peptides containing DRAHYNI proliferated and produced interleukin when challenged vitro peptide or whole HPV-16 fusion protein. The T epitope was recognized association all five histocompatibility complex class II I-A I-E alleles tested. Synthetic consisting linked to B-cell...
Background Over 14 million people die each year from infectious diseases despite extensive vaccine use [1]. The needle and syringe—first invented in 1853—is still the primary delivery device, injecting liquid into muscle. Vaccines could be far more effective if they were precisely delivered narrow layer just beneath skin surface that contains a much higher density of potent antigen-presenting cells (APCs) essential to generate protective immune response. We hypothesized successful...
Abstract The ‘Nanopatch’ (NP) comprises arrays of densely packed projections with a defined geometry and distribution designed to physically target vaccines directly thousands epidermal dermal antigen presenting cells (APCs). These miniaturized are two orders magnitude smaller than standard needles—which deliver most vaccines—and also much current microneedle arrays. NP is dry‐coated antigen, adjuvant, and/or DNA payloads. After the was pressed onto mouse skin, protein payload co‐localized...
Abstract Targeting of vaccines to abundant immune cell populations within our outer thin skin layers using miniaturized devices—much thinner than a needle and syringe, could improve the efficacy (and other immunotherapies). To meet this goal, densely packed dissolving microprojection array (dissolving Nanopatch) is designed, achieving functional miniaturization by 1) formulating small microneedles (two orders magnitude smaller standard syringe) 2) multiple layering payload microprojections...
Injection using needle and syringe (N&S) is the most widely used method for vaccination, but requires trained healthcare workers. Fear of needles, risk needle-stick injury, need to reconstitute lyophilised vaccines, are also drawbacks. The Nanopatch (NP) a microarray skin patch comprised high-density array microprojections dry-coated with vaccine that being developed address these shortcomings. Here we report randomised, partly-blinded, placebo-controlled trial represents first use in humans...
Background The Vaxxas high-density microarray patch (HD-MAP) consists of a high density microprojections coated with vaccine for delivery into the skin. Microarray patches (MAPs) offer possibility improved thermostability as well potential to be safer, more acceptable, easier use, and cost-effective administration vaccines than injection by needle syringe (N&S). Here, we report phase I trial using HD-MAP deliver monovalent influenza that was best our knowledge first clinical evaluate safety,...
Development of CD8 alphabeta CTL epitope-based vaccines requires an effective strategy capable co-delivering large numbers epitopes. Here we describe a DNA plasmid encoding polyepitope or "polytope" protein, which contained multiple contiguous minimal murine Mice vaccinated with this made MHC-restricted responses to each the epitopes, and protective were demonstrated in recombinant vaccinia virus, influenza tumor challenge models. generated by polytope vaccination lasted for 1 yr, could be...
The lipids of rat milk, the contents 9-10-dayold stomach and intestine, lymph, plasma, liver were quantitated their fatty acids analyzed.Rat milk consists 97% triacylglycerols, which 35% are medium chain length (C8-C12).However, triacylglycerols show a 25% reduction in acids, indicates preferential hydrolysis stomach.The intestinal lumen free acid composition shows decreased compared to long indicating absorption former.Lymph was shown contain significant amount (-22%) acids.The content vena...
The amino acid sequence of an insect apolipoprotein, apolipophorin-I11 from Manduca sexta, was determined by a combination cDNA and protein sequencing.The mature hemolymph consists 166 acids.The also encodes for aminoterminal extension 23 acids which is not represented in the protein.The existence precursor confirmed vitro translation fat body mRNA.Computer-assisted comparative analysis revealed following points: 1) composed tandemly repeating tetradecapeptide units with high potential...
Background Better delivery systems are needed for routinely used vaccines, to improve vaccine uptake. Many vaccines contain alum or based adjuvants. Here we investigate a novel dry-coated densely-packed micro-projection array skin patch (Nanopatch™) as an alternate system intramuscular injection delivering adjuvanted human papillomavirus (HPV) (Gardasil®) commonly prophylactic against cervical cancer. Methodology/Principal Findings Micro-projection arrays with material delivered C57BL/6...
The disadvantages of needle-based immunisation motivate the development simple, low cost, needle-free alternatives. Vaccine delivery to cutaneous environments rich in specialised antigen-presenting cells using microprojection patches has practical and immunological advantages over conventional needle delivery. Additionally, stable coating vaccine onto microprojections removes logistical obstacles presented by strict requirement for cold-chain storage distribution liquid vaccine, or...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 160 million people and resulted in 3.3 deaths, despite the availability of multiple vaccines, world still faces many challenges with their rollout. Here, we use high-density microarray patch (HD-MAP) to deliver a SARS-CoV-2 spike subunit vaccine directly skin. We show that is thermostable on patches, delivery enhancing both cellular antibody immune responses. Elicited antibodies potently neutralize clinically...
The ongoing SARS-CoV-2 pandemic continues to be a major health burden globally, especially in resource-limited areas. Continued research into more effective and accessible vaccines is required reduce the of disease. Here, we use an emerging vaccine delivery system, high-density microarray patch (HD-MAP) deliver plasmid DNA (Delta 6P) encoding for spike protein. HD-MAP this resulted robust IgG responses mice against multiple domains cellular response vaccination was also measured, comparative...
Importance High-resolution microultrasonography-guided biopsy is an alternative to MRI fusion-guided for prostate cancer diagnosis. Objective To compare and biopsy. Design, Setting, Participants A multicenter, international, open-label, randomized, noninferiority trial of biopsy-naive men from 20 centers (8 countries) with clinical suspicion (elevated prostate-specific antigen [PSA] and/or abnormal digital rectal examination findings) December 2021 September 2024. Interventions were assigned...