A5028119025- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virology and Viral Diseases
- Vector-borne infectious diseases
- Reproductive Physiology in Livestock
- Viral Infections and Outbreaks Research
- CRISPR and Genetic Engineering
- Single-cell and spatial transcriptomics
- Genetic and phenotypic traits in livestock
- HIV Research and Treatment
- Heat shock proteins research
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Advancements in Transdermal Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Toxin Mechanisms and Immunotoxins
The University of Queensland
2018-2025
New York Genome Center
2024
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 160 million people and resulted in 3.3 deaths, despite the availability of multiple vaccines, world still faces many challenges with their rollout. Here, we use high-density microarray patch (HD-MAP) to deliver a SARS-CoV-2 spike subunit vaccine directly skin. We show that is thermostable on patches, delivery enhancing both cellular antibody immune responses. Elicited antibodies potently neutralize clinically...
The ongoing SARS-CoV-2 pandemic continues to be a major health burden globally, especially in resource-limited areas. Continued research into more effective and accessible vaccines is required reduce the of disease. Here, we use an emerging vaccine delivery system, high-density microarray patch (HD-MAP) deliver plasmid DNA (Delta 6P) encoding for spike protein. HD-MAP this resulted robust IgG responses mice against multiple domains cellular response vaccination was also measured, comparative...
Dengue virus (DENV) is a major health concern throughout the world infecting up to 390 million people globally each year. Infection with any one of four DENV serotypes produces spectrum clinical illness ranging from mild undifferentiated febrile disease through severe dengue involving fever and haemorrhage. There currently no antiviral treatment for only licensed vaccine limited distribution. This study characterises kinetics serological biomarker, NS1, appearance anti-NS1 IgG, anti-E IgM...
Dengue viruses (DENV) cause an estimated 390 million infections globally. With no dengue-specific therapeutic treatment currently available, vaccination is the most promising strategy for its control. A wide range of DENV vaccines are in development, with one having already been licensed, albeit limited distribution. We investigated immunogenicity and protective efficacy a chimeric virus vaccine candidate based on insect-specific flavivirus, Binjari (BinJV), displaying structural prM/E...
Since the start of COVID-19 pandemic, multiple waves SARS-CoV-2 variants have emerged. Of particular concern is omicron variant, which harbors 28 mutations in spike glycoprotein receptor binding and N-terminal domains relative to ancestral strain. The high mutability therefore poses significant hurdles for development universal assays that rely on spike-specific immune detection. To address this, more conserved viral antigens need be targeted. In this work, we comprehensively demonstrate use...
The COVID-19 pandemic has highlighted the need for vaccines capable of providing rapid and robust protection. One way to improve vaccine efficacy is delivery via microarray patches, such as Vaxxas high-density patch (HD-MAP). We have previously demonstrated that a SARS-CoV-2 protein candidate, HexaPro, HD-MAP induces potent humoral immune responses. Here, we investigate cellular responses induced by HexaPro vaccination. found type one biassed response much greater magnitude compared standard...
Summary Progesterone signaling and uterine function are crucial in terms of pregnancy establishment. To investigate how the tissue its secretion changes relation to puberty, we sampled fluid from six pre‐ post‐pubertal Brahman heifers. Post‐pubertal heifers were luteal phase. Gene expression was investigated with RNA ‐sequencing, whereas used for protein profiling mass spectrometry. A total 4034 genes differentially expressed ( DE ) at a nominal P ‐value 0.05, 26 significantly after...
The ongoing coronavirus disease 2019 (COVID-19) pandemic continues to disrupt essential health services in 90 percent of countries today. spike (S) protein found on the surface causative agent, SARS-CoV-2 virus, has been prime target for current vaccine research since antibodies directed against S were neutralize virus. However, as new variants emerge, mutations within have given rise potential immune evasion response generated by generation vaccines. In this study, a modified, HexaPro...
The ongoing SARS-CoV-2 pandemic continues to pose an enormous health challenge globally. emergence of variants concern has resulted in decreased vaccine efficacy necessitating booster immunizations. This was particularly highlighted by the recent Omicron variant, which contains over 30 mutations spike protein and quickly became dominant viral strain global circulation. We previously demonstrated that delivery a subunit via high-density microarray patch (HD-MAP) induced potent immunity...
Alternative delivery systems such as the high-density microarray patch (HD-MAP) are being widely explored due to variety of benefits they offer over traditional vaccine methods. As vaccines dry coated onto HD-MAP, there is a need ensure stability in solid state upon down. Other challenges faced structural during storage dried and reconstitution application into skin. Using novel live chimeric virus candidate, BinJ/DENV2-prME, we panel pharmaceutical excipients mitigate loss drying process....
Abstract SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 deaths, we still face many challenges the rollout of vaccines. Here, use high-density microarray patch to deliver a spike subunit vaccine directly skin. We show vaccine, dry-coated on is thermostable, delivery via HD-MAP induced greater cellular antibody immune responses, with serum able potently neutralize clinically relevant isolates including those from B.1.1.7 B.1.351 lineages. Finally, single dose...
Zika virus (ZIKV) infections are spreading silently with limited global surveillance in at least 89 countries and territories. There is a pressing need to develop an effective vaccine suitable for equitable distribution globally. Consequently, we previously developed proprietary DNA encoding secreted non-structural protein 1 of ZIKV (pVAX-tpaNS1) elicit rapid protection T cell-dependent manner mice. In the current study, evaluated stability, efficacy, immunogenicity delivering this into skin...