A5037368909- Streptococcal Infections and Treatments
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Neonatal and Maternal Infections
- Bacterial Infections and Vaccines
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Computational Drug Discovery Methods
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Forensic Toxicology and Drug Analysis
- Infections and bacterial resistance
- HIV Research and Treatment
The University of Queensland
2019-2025
To be optimally effective, peptide-based vaccines need to administered with adjuvants. Many currently available adjuvants are toxic, not biodegradable; they invariably invoke adverse reactions, including allergic responses and excessive inflammation. A nontoxic, biodegradable, biocompatible, self-adjuvanting vaccine delivery system is urgently needed. Herein, we report a potent fulfilling the above requirements. peptide antigen was coupled poly-hydrophobic amino acid sequences serving as...
Peptide antigens have been widely used in the development of vaccines, especially for those against autoimmunity-inducing pathogens and cancers. However, peptide-based vaccines require adjuvant and/or a delivery system to stimulate desired immune responses. Here, we explored potential self-adjuvanting poly(hydrophobic amino acids) (pHAAs) deliver vaccine Group A Streptococcus (GAS). We designed synthesized self-assembled nanoparticles with variety conjugates bearing peptide antigen...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected more than 160 million people and resulted in 3.3 deaths, despite the availability of multiple vaccines, world still faces many challenges with their rollout. Here, we use high-density microarray patch (HD-MAP) to deliver a SARS-CoV-2 spike subunit vaccine directly skin. We show that is thermostable on patches, delivery enhancing both cellular antibody immune responses. Elicited antibodies potently neutralize clinically...
Immune stimulants (adjuvants) are essential vaccine components; however, clinically approved adjuvants limited with the majority being derived from pathogenic components. In this study, adjuvanting capacity of cholesterol, a natural human lipid, was explored following conjugation peptide antigens. A structure-activity relationship study conducted to compare linear and branched cholesterol conjugates other lipopeptide vaccines commercial adjuvants. Group Streptococcus (GAS) M protein-derived...
Peptide-based vaccine development represents a highly promising strategy for preventing Group A Streptococcus (GAS) infection. However, these vaccines need to be administered with the help of delivery system and/or immune adjuvant. Cell-penetrating peptides (CPPs) have been used as powerful tool delivering various therapeutic agents, including peptides, they can overcome permeability barrier cell membranes. Here, we CPPs deliver our lead lipopeptide-based (LCP-1). were anchored through...
Peptide-based subunit vaccines require an immunostimulant (adjuvant) and/or delivery system to protect the antigenic peptide from degradation and induce desired immunity. Currently available adjuvants are either too toxic for human use (experimental adjuvants) or they limited in particular licensed countries (commercial adjuvants). Therefore, there is immediate need novel that both safe effective. Herein, we assessed ability of cholic acid (a major bile acid) as a nontoxic, biodegradable,...
The ongoing coronavirus disease 2019 (COVID-19) pandemic continues to disrupt essential health services in 90 percent of countries today. spike (S) protein found on the surface causative agent, SARS-CoV-2 virus, has been prime target for current vaccine research since antibodies directed against S were neutralize virus. However, as new variants emerge, mutations within have given rise potential immune evasion response generated by generation vaccines. In this study, a modified, HexaPro...
The ongoing SARS-CoV-2 pandemic continues to pose an enormous health challenge globally. emergence of variants concern has resulted in decreased vaccine efficacy necessitating booster immunizations. This was particularly highlighted by the recent Omicron variant, which contains over 30 mutations spike protein and quickly became dominant viral strain global circulation. We previously demonstrated that delivery a subunit via high-density microarray patch (HD-MAP) induced potent immunity...
The SARS-CoV-2 virus has caused a global crisis, resulting in 0.5 billion infections and over 6 million deaths as of March 2022. Fortunately, infection hospitalization rates were curbed due to the rollout DNA mRNA vaccines. However, efficacy these vaccines significantly drops few months post immunization, from 88% down 47% case Pfizer BNT162 vaccine. emergence variant strains, especially delta omicron, have also reduced vaccine efficacy. We propose peptide potential solution address...
Abstract SARS-CoV-2 has infected over 160 million people and resulted in more than 3.3 deaths, we still face many challenges the rollout of vaccines. Here, use high-density microarray patch to deliver a spike subunit vaccine directly skin. We show vaccine, dry-coated on is thermostable, delivery via HD-MAP induced greater cellular antibody immune responses, with serum able potently neutralize clinically relevant isolates including those from B.1.1.7 B.1.351 lineages. Finally, single dose...
Adjuvants and delivery systems are essential components of vaccines to increase immunogenicity against target antigens, particularly for peptide epitopes (poor immunogens). Emulsions, nanoparticles, liposomes commonly used as a system peptide-based vaccines. A Poly(hydrophobic amino acids) was previously conjugated Group Streptococcus (GAS)-derived epitopes, allowing the conjugates self-assemble into nanoparticles with self adjuvanting ability. Their hydrophobic acid tail also serves an...
Peptide-based vaccines are composed of small, defined, antigenic peptide epitopes. They designed to induce well-controlled immune responses. Multiple epitopes often employed in these cover strain variability a pathogen. However, cannot stimulate adequate responses on their own and require an adjuvant (immune stimulant) and/or delivery system. Here, we synthesized multiepitope vaccine candidate against Group A Streptococcus (GAS) several B-cell (J8, PL1, 88/30) derived from GAS M-protein,...
This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum. The requires a small amount S-protein/RBD and angiotensin converting enzyme-2 (ACE2). A high-throughput, simple ELISA technique is employed. Plate-coated-RBDs are allowed to interact with the serum, then soluble ACE2 added, followed secondary antibodies substrate. key steps in this include (1) serum heat treatment...
Cocaine is one of the most widely used and increasingly popular illicit psychoactive drugs. Unlike other commonly substances abuse, cocaine has no pharmacological therapies to treat addiction or aid in rehabilitation. Immunopharmacology long been touted as a possible avenue develop effective anticocaine therapies; however, lack efficacy designs which are not consistent with simple large-scale production have hindered vaccine translation. We designed synthesized peptide-based anti-cocaine...
Abstract This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum. The requires a small amount S-protein/RBD and angiotensin-converting enzyme-2 (ACE2). A high-throughput, simple ELISA technique is employed. Plate-coated-RBDs are allowed to interact with the serum, then soluble ACE2 added, followed secondary antibodies substrate. key steps in this include: 1) serum heat...
Most vaccines require co-delivery of an adjuvant in order to generate the desired immune responses. However, many currently available adjuvants are non-biodegradable, have limited efficacy, and/or poor safety profile. Thus, new adjuvants, or self-adjuvanting vaccine delivery systems, required. Here, we proposed a system that is fully defined, biodegradable, and non-toxic. The produced by conjugation polyleucine peptide antigen, followed self-assembly conjugate into nanoparticles. protocol...