A5062887582- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Natural Antidiabetic Agents Studies
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Reproductive System and Pregnancy
- Algal biology and biofuel production
- Malaria Research and Control
- Alzheimer's disease research and treatments
- Drug Transport and Resistance Mechanisms
- HIV Research and Treatment
- Invertebrate Immune Response Mechanisms
- vaccines and immunoinformatics approaches
- Seaweed-derived Bioactive Compounds
- Animal Virus Infections Studies
- Phytochemistry and Biological Activities
- Forensic Toxicology and Drug Analysis
- melanin and skin pigmentation
The University of Queensland
2020-2025
Pukyong National University
2017-2018
Abstract Antigens incorporated in subunit vaccines are typically poorly immunogenic, so a strong immunostimulant (adjuvant) and/or delivery system is required to boost immunogenicity. In this work, the various functional polymer nanostructures, that is, rods, worms, spheres, and tadpoles used develop potent peptide antigen systems. The PADRE‐J8 (PJ8), derived from Group A Streptococcus (GAS) M‐protein, either physically mixed or chemically conjugated polymeric nanoparticles of different...
Mucosal vaccines are highly attractive due to high patient compliance and their suitability for mass immunizations. However, all currently licensed mucosal composed of attenuated/inactive whole microbes, which associated with a variety safety concerns. In contrast, modern subunit use minimal pathogenic components (antigens) that safe but typically poorly immunogenic when delivered via administration. this study, we demonstrated the utility various functional polymer-based nanostructures as...
Abstract Cyclic peptides are often used as scaffolds for the multivalent presentation of drug molecules due to their structural stability and constrained conformation. We identified a cyclic deca-peptide incorporating lipoamino acids delivering T helper B cell epitopes against group A Streptococcus (GAS), eliciting robust humoral immune responses. In this study, we assessed function-immunogenicity relationship multi-component vaccine candidate (referred VC-13) elucidate mechanism action....
Immune stimulants (adjuvants) are essential vaccine components; however, clinically approved adjuvants limited with the majority being derived from pathogenic components. In this study, adjuvanting capacity of cholesterol, a natural human lipid, was explored following conjugation peptide antigens. A structure-activity relationship study conducted to compare linear and branched cholesterol conjugates other lipopeptide vaccines commercial adjuvants. Group Streptococcus (GAS) M protein-derived...
To discover lead lupane triterpenoid's potential isolated from Pueraria lobata roots against β-site amyloid precursor protein cleaving enzyme 1 (BACE1), which serve as a rate limiting step in beta (Aβ) production altering the course of Alzheimer's disease. In addition, kinetics study and molecular docking were conducted to establish inhibition type structure activity relationship.A systematic 70% ethanolic P. root extract was employed identify its BACE1 inhibitory potential. Further, two...
Human papilloma virus (HPV) is responsible for all cases of cervical cancer. While prophylactic vaccines are available, the development peptide-based as a therapeutic strategy still under investigation. In comparison with traditional and currently used treatment strategies chemotherapy surgery, vaccination against HPV promising option fewer side effects. A peptide derived from HPV-16 E7 protein, called 8Qm, in combination adjuvants showed promise vaccine. Here, ability polymerized natural...
Kuwanon G (KG) and benzofuran flavonoids such as mulberrofuran (MG) albanol B (AB) isolated from Morus sp. are reported to exhibit anti-Alzheimer’s disease, anti-inflammatory, fungicidal, anti-cancer, anti-bacterial, anti-tyrosinase properties. We investigated the inhibition of mono- diphenolase activity mushroom tyrosinase by KG, MG, AB. KG MG displayed acceptable compared kojic acid. AB did not show any up 350 µM. six-fold higher l-tyrosine oxidation (IC50 = 6.35 ± 0.45 µM) acid 36.0 µM)....
Peptide-based vaccines can trigger highly specific immune responses, although peptides alone are usually unable to confer strong humoral or cellular immunity. Consequently, peptide antigens administered with immunostimulatory adjuvants, but only a few safe and effective for human use. To overcome this obstacle, herein antigen was lipidated effectively anchor it liposomes emulsion. A B cell epitope from Group Streptococcus M protein conjugated universal T helper epitope, the pan DR-biding...
Subunit vaccines require an immunostimulant (adjuvant) and/or delivery system to induce immunity. However, currently, available adjuvants are either too dangerous in terms of side effects for human use (experimental adjuvants) or have limited efficacy and applicability. In this study, we examined the capacity mannose-lipopeptide ligands enhance immunogenicity a vaccine consisting polyleucine(L15)-antigen conjugates anchored liposomes. The clinically tested Group A Streptococcus (GAS) B-cell...
The current study assesses the antioxidant effects of two similar isoflavonoids isolated from Pueraria lobata, coumestrol and puerarol, along with cholinergic amyloid-cascade pathways to mitigate Alzheimer's disease (AD). Antioxidant activity was evaluated via 1,1-diphenyl-2-picryhydrazyl (DPPH) peroxynitrite (ONOO-) scavenging ability further screened ONOO--mediated nitrotyrosine. Similarly, acetyl- butyrylcholinesterase (AChE/BChE) β-site amyloid precursor protein cleaving enzyme 1 (BACE1)...
Adjuvants and delivery systems are essential components of vaccines to increase immunogenicity against target antigens, particularly for peptide epitopes (poor immunogens). Emulsions, nanoparticles, liposomes commonly used as a system peptide-based vaccines. A Poly(hydrophobic amino acids) was previously conjugated Group Streptococcus (GAS)-derived epitopes, allowing the conjugates self-assemble into nanoparticles with self adjuvanting ability. Their hydrophobic acid tail also serves an...
Contraceptive vaccines are designed to elicit immune responses against major components of animal reproductive systems. These vaccines, which most commonly administered via injection, typically target gonadotropin-releasing hormone (GnRH). However, the need restrain animals for treatment limits field applications injectable vaccines. Oral administration would broaden vaccine applicability. We explored contraceptive candidates composed GnRH peptide hormone, universal T helper PADRE (P), and a...
The presence of a cyclic peptide is as part physically-mixed group A Streptococcus vaccine for the induction strong, balanced Th1/Th2 immune response.
Porcine circovirus 2 (PCV2) infection is one of the most serious threats to swine industry. While disease can be prevented, some extent, by commercial PCV2a vaccines, evolving nature PCV2 necessitates development a novel vaccine that compete with mutations virus. Thus, we have developed multiepitope vaccines based on PCV2b variant. Three capsid protein epitopes, together universal T helper epitope, were synthesized and formulated five delivery systems/adjuvants: complete Freund's adjuvant,...
Untreated group A Streptococcus (GAS) can lead to a range of life-threatening diseases, including rheumatic heart disease. To date, no therapeutic or prophylactic vaccines are commercially available treat prevent GAS infection. Development peptide-based subunit vaccine offers promising solution, negating the safety issues live-attenuated inactive vaccines. Subunit administer small peptide fragments (antigens), which typically poorly immunogenic. Therefore, these antigens require formulation...
Cocaine is one of the most widely used and increasingly popular illicit psychoactive drugs. Unlike other commonly substances abuse, cocaine has no pharmacological therapies to treat addiction or aid in rehabilitation. Immunopharmacology long been touted as a possible avenue develop effective anticocaine therapies; however, lack efficacy designs which are not consistent with simple large-scale production have hindered vaccine translation. We designed synthesized peptide-based anti-cocaine...
Peptide-based subunit vaccines include only minimal antigenic determinants, and, therefore, are less likely to induce allergic immune responses and adverse effects compared traditional vaccines. However, peptides weakly immunogenic susceptible enzymatic degradation when administered on their own. Hence, we designed polyelectrolyte complex (PEC)-based delivery systems protect peptide antigens from improve immunogenicity. Lipopeptide (LCP-1) bearing J8 B-cell epitope derived Group A...
The aim of the present study was to evaluate ability raw mini tomato (Solanum lycopersicum L.) juice powder attenuate cytotoxicity in combination with powders from two orange species [Citrus sinensis (L.) Osbeck and Citrus unshiu Marcow]. To this end, hepatoprotective activities these combinations against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress HepG2 cells were evaluated, most suitable ratios for optimal flavonoid availability identified. levels intracellular reactive...
Malaria is a vector born parasitic disease causing millions of deaths every year. Despite the high mortality rate, an effective vaccine against this mosquito-borne infectious yet to be developed. Up date, RTS,S/AS01 only available for malaria prevention; however, its efficacy low. Among variety antigens, merozoite surface protein-1(MSP-1) and ring-infected erythrocyte antigen (RESA) have been proposed as promising candidates development. We developed peptide-based Plasmodium falciparum that...