A5086825190- Streptococcal Infections and Treatments
- Inhalation and Respiratory Drug Delivery
- Parasites and Host Interactions
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Neonatal and Maternal Infections
- Parasite Biology and Host Interactions
- Microencapsulation and Drying Processes
- Helminth infection and control
- Pneumonia and Respiratory Infections
- SARS-CoV-2 detection and testing
- Aerosol Filtration and Electrostatic Precipitation
- vaccines and immunoinformatics approaches
- Granular flow and fluidized beds
- Combustion and Detonation Processes
- Bacterial Infections and Vaccines
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Antimicrobial Resistance in Staphylococcus
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Computational Drug Discovery Methods
- Click Chemistry and Applications
- Glycosylation and Glycoproteins Research
The University of Queensland
2021-2025
Peptide antigens have been widely used in the development of vaccines, especially for those against autoimmunity-inducing pathogens and cancers. However, peptide-based vaccines require adjuvant and/or a delivery system to stimulate desired immune responses. Here, we explored potential self-adjuvanting poly(hydrophobic amino acids) (pHAAs) deliver vaccine Group A Streptococcus (GAS). We designed synthesized self-assembled nanoparticles with variety conjugates bearing peptide antigen...
Abstract Antigens incorporated in subunit vaccines are typically poorly immunogenic, so a strong immunostimulant (adjuvant) and/or delivery system is required to boost immunogenicity. In this work, the various functional polymer nanostructures, that is, rods, worms, spheres, and tadpoles used develop potent peptide antigen systems. The PADRE‐J8 (PJ8), derived from Group A Streptococcus (GAS) M‐protein, either physically mixed or chemically conjugated polymeric nanoparticles of different...
Mucosal vaccines are highly attractive due to high patient compliance and their suitability for mass immunizations. However, all currently licensed mucosal composed of attenuated/inactive whole microbes, which associated with a variety safety concerns. In contrast, modern subunit use minimal pathogenic components (antigens) that safe but typically poorly immunogenic when delivered via administration. this study, we demonstrated the utility various functional polymer-based nanostructures as...
Abstract Cyclic peptides are often used as scaffolds for the multivalent presentation of drug molecules due to their structural stability and constrained conformation. We identified a cyclic deca-peptide incorporating lipoamino acids delivering T helper B cell epitopes against group A Streptococcus (GAS), eliciting robust humoral immune responses. In this study, we assessed function-immunogenicity relationship multi-component vaccine candidate (referred VC-13) elucidate mechanism action....
Immune stimulants (adjuvants) are essential vaccine components; however, clinically approved adjuvants limited with the majority being derived from pathogenic components. In this study, adjuvanting capacity of cholesterol, a natural human lipid, was explored following conjugation peptide antigens. A structure-activity relationship study conducted to compare linear and branched cholesterol conjugates other lipopeptide vaccines commercial adjuvants. Group Streptococcus (GAS) M protein-derived...
Immunocontraceptive vaccines targeting gonadotropin-releasing hormone (GnRH) are in high demand for controlling population growth and managing the temperament of both wild domesticated animals. Achieving adequate efficacy, especially with a single-dose immunization, requires potent adjuvants. However, commercial face challenges: some, like Gonacon®, have regulatory issues veterinary authorities, while others, Bopriva®, lack potency require multiple booster doses. Thus, there is critical need...
A major COVID-19 vaccine strategy is to induce antibodies that prevent interaction between the Spike protein's receptor-binding domain (RBD) and angiotensin-converting enzyme 2 (ACE2). These vaccines will also T-cell responses. However, concerns were raised aberrant vaccine-induced immune responses may exacerbate disease. We aimed identify minimal epitopes on RBD would antibody block of ACE2 as a leading an effective with reduced risk inducing immunopathology.We procured series overlapping...
Peptide-based vaccines can trigger highly specific immune responses, although peptides alone are usually unable to confer strong humoral or cellular immunity. Consequently, peptide antigens administered with immunostimulatory adjuvants, but only a few safe and effective for human use. To overcome this obstacle, herein antigen was lipidated effectively anchor it liposomes emulsion. A B cell epitope from Group Streptococcus M protein conjugated universal T helper epitope, the pan DR-biding...
Subunit vaccines require an immunostimulant (adjuvant) and/or delivery system to induce immunity. However, currently, available adjuvants are either too dangerous in terms of side effects for human use (experimental adjuvants) or have limited efficacy and applicability. In this study, we examined the capacity mannose-lipopeptide ligands enhance immunogenicity a vaccine consisting polyleucine(L15)-antigen conjugates anchored liposomes. The clinically tested Group A Streptococcus (GAS) B-cell...
Approximately 0.4 billion individuals worldwide are infected with hookworm. An effective vaccine is needed to not only improve the health of those affected and at high risk, but also economic growth in disease-endemic areas. ideal anti-hookworm therapeutic strategy for mass administration a stable orally administered vaccine. Oral vaccines advantageous as they negate need trained medical staff do require strict sterility conditions. Vaccination, therefore, can be carried out significantly...
The SARS-CoV-2 virus has caused a global crisis, resulting in 0.5 billion infections and over 6 million deaths as of March 2022. Fortunately, infection hospitalization rates were curbed due to the rollout DNA mRNA vaccines. However, efficacy these vaccines significantly drops few months post immunization, from 88% down 47% case Pfizer BNT162 vaccine. emergence variant strains, especially delta omicron, have also reduced vaccine efficacy. We propose peptide potential solution address...
This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum. The requires a small amount S-protein/RBD and angiotensin converting enzyme-2 (ACE2). A high-throughput, simple ELISA technique is employed. Plate-coated-RBDs are allowed to interact with the serum, then soluble ACE2 added, followed secondary antibodies substrate. key steps in this include (1) serum heat treatment...
Untreated group A Streptococcus (GAS) can lead to a range of life-threatening diseases, including rheumatic heart disease. To date, no therapeutic or prophylactic vaccines are commercially available treat prevent GAS infection. Development peptide-based subunit vaccine offers promising solution, negating the safety issues live-attenuated inactive vaccines. Subunit administer small peptide fragments (antigens), which typically poorly immunogenic. Therefore, these antigens require formulation...
Abstract COVID‐19 pandemic has been the deadliest infectious disease outbreak since Spanish flu. The emerging variant lineages, decay of neutralizing antibodies, and occur reinfections require development highly protective safe vaccines. As currently approved vaccines that utilize virus‐related genetic material are less than ideal, other vaccine types have also widely investigated. Among them, peptide‐based hold great promise in countering as they may overcome most shortcomings RNA/DNA...