A5046282420- Microbial Natural Products and Biosynthesis
- Streptococcal Infections and Treatments
- Marine Sponges and Natural Products
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Neuropeptides and Animal Physiology
- Neonatal and Maternal Infections
- Pain Mechanisms and Treatments
- Receptor Mechanisms and Signaling
- Synthetic Organic Chemistry Methods
- Synthesis and Biological Activity
- Antimicrobial Peptides and Activities
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Pneumonia and Respiratory Infections
- Antimicrobial Resistance in Staphylococcus
- Nicotinic Acetylcholine Receptors Study
- Chemical synthesis and alkaloids
- Chemical Synthesis and Analysis
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Microbial Community Ecology and Physiology
- Nitric Oxide and Endothelin Effects
- Alkaloids: synthesis and pharmacology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
The University of Queensland
2016-2025
Cairo University
2022
Griffith University
2022
Translational Research Institute
2019-2020
Princess Alexandra Hospital
2016
Brisbane School of Theology
2015
Austin Health
2009-2011
Heidelberg Repatriation Hospital
2009-2011
Heidelberg University
2011
University Hospital Heidelberg
2011
To be optimally effective, peptide-based vaccines need to administered with adjuvants. Many currently available adjuvants are toxic, not biodegradable; they invariably invoke adverse reactions, including allergic responses and excessive inflammation. A nontoxic, biodegradable, biocompatible, self-adjuvanting vaccine delivery system is urgently needed. Herein, we report a potent fulfilling the above requirements. peptide antigen was coupled poly-hydrophobic amino acid sequences serving as...
We validate the improved resolution and sensitivity of C3 Marfey's method, including an ability to resolve all Ile isomers, against array amino acids commonly encountered in natural products by comparison existing method. also describe innovative 2D method as analytical approach for determining regiochemistry enantiomeric acid residues products. The methods represent valuable tools probing defining stereocomplexity hydrolytically accessible
We describe the identification of a conopeptide sequence in venom duct mRNA from Conus victoriae that suppresses vascular response to pain rat. PCR-RACE was used screen cDNAs for those transcripts encode specific antagonists vertebrate neuronal nicotinic acetylcholine receptors (nAChRs). One these peptides, Vc1.1, active as an antagonist nAChRs receptor binding and functional studies bovine chromaffin cells. It also suppressed responses unmyelinated sensory nerve C-fiber activation rats....
We previously reported that the time frame and extent of changes in peripheral neurogenic inflammatory response skin area, innervated by an injured nerve, coincide with those pain behaviours. raised possibility common factors might operate to modulate neuropathic processes rats chronic constriction nerve injury (CCI). In present study we examined role free radicals modulating vascular thermal hyperalgesia CCI sciatic nerve. Free radicals, via interaction nitric oxide (NO) form peroxynitrite,...
Fractionation of a southern Australian marine sponge, Ianthella sp., yielded sixteen metabolites including new class pyrrolidone, ianthellidones A–F (1–6), furanone, G–H (7–8), and known lamellarins, lamellarins O1 (9), O2 (10), O (11) Q (12), plus the 4-hydroxybenzaldehyde (13), 4-hydroxybenzoic acid (14), 4-methoxybenzoic (15) ethyl 4-hydroxybenzoate (16). Structures for all were determined by detailed spectroscopic analysis, supported plausible biosynthetic relationship. The non-cytotoxic...
Peptide antigens have been widely used in the development of vaccines, especially for those against autoimmunity-inducing pathogens and cancers. However, peptide-based vaccines require adjuvant and/or a delivery system to stimulate desired immune responses. Here, we explored potential self-adjuvanting poly(hydrophobic amino acids) (pHAAs) deliver vaccine Group A Streptococcus (GAS). We designed synthesized self-assembled nanoparticles with variety conjugates bearing peptide antigen...
An intact nociceptor system of primary afferent sensory nerves is important for the initiation inflammatory process and successful tissue repair. Dysfunction this with age could be a contributing factor delayed wound healing in elderly. This study was designed to examine role (using capsaicinpretreated rats) effect aging on thermal induced interscapular region CO2 laser). The endpoint time when full contraction had occurred. ability peptides, substance P (SP) calcitonin gene-related peptide...
Abstract Chemical analysis of a Didemnum sp. (CMB‐01656) collected during scientific Scuba operations off Wasp Island, New South Wales, yielded five new lamellarins A1 ( 1 ), A2 2 A3 3 A4 4 ) and A5 5 eight known C 6 E 7 K 8 M 9 S 10 T 11 X 12 χ 13 ). Analysis second (CMB‐02127) trawling along the Northern Rottnest Shelf, Western Australia, lamellarin A6 14 two G 15 Z 16 Structures were assigned to , on basis detailed spectroscopic with comparison literature data authentic samples. Access...
Chemical analysis of a marine-derived Streptomyces sp. (CMB-M0423) isolated from beach sand off Heron Island, Australia, yielded three new members the rare pyrroloterpene biosynthetic structure class. Identified by detailed spectroscopic as first reported examples naturally occurring 2-nitropyrroles, heronapyrroles A−C (1−3) displayed promising biological activity—with low to submicromolar IC50 activity against Gram-positive bacteria but no cytotoxicity toward mammalian cell lines.
To develop novel polymer-based nanoscale delivery system for lipopeptide-based vaccine against group A Streptococcus (GAS).Four types of lipopeptide antigen-loaded polymeric nanoparticles (NP) were prepared. NP accessed their capacity to be taken up by dendritic cells; effect on cell maturation; ability induce mucosal and systemic immunity; capability antibody responses that opsonize GAS bacteria.The combination adjuvanting properties lipopeptides dextran/trimethyl chitosan-based had a...
A soil Streptomyces nov. sp. (MST-115088) isolated from semiarid terrain near Wollogorang Station, Queensland, returned two known and new examples of a rare class cyclic hexapeptide, desotamides B (1 2) E F (3 4), respectively, together with d-Orn homologues, wollamides (5 6). Structures were assigned by detailed spectroscopic C3 Marfey's analysis. The desotamides/wollamides exhibit growth inhibitory activity against Gram-positive bacteria (IC50 0.6–7 μM) are noncytotoxic to mammalian cells...
Chemical analysis of an Australian marine sediment-derived Aspergillus sp. (CMB-M081F) yielded the new diketomorpholine (DKM) shornephine A (1) together with two known and one diketopiperazine (DKP), 15b-β-hydroxy-5-N-acetyladreemin (2), 5-N-acetyladreemin (3), 15b-β-methoxy-5-N-acetyladreemin (4), respectively. Structure elucidation 1–4 was achieved by detailed spectroscopic analysis, supported chemical degradation derivatization, biosynthetic considerations. The DKM underwent a facile...
Abstract Antigens incorporated in subunit vaccines are typically poorly immunogenic, so a strong immunostimulant (adjuvant) and/or delivery system is required to boost immunogenicity. In this work, the various functional polymer nanostructures, that is, rods, worms, spheres, and tadpoles used develop potent peptide antigen systems. The PADRE‐J8 (PJ8), derived from Group A Streptococcus (GAS) M‐protein, either physically mixed or chemically conjugated polymeric nanoparticles of different...
Mucosal vaccines are highly attractive due to high patient compliance and their suitability for mass immunizations. However, all currently licensed mucosal composed of attenuated/inactive whole microbes, which associated with a variety safety concerns. In contrast, modern subunit use minimal pathogenic components (antigens) that safe but typically poorly immunogenic when delivered via administration. this study, we demonstrated the utility various functional polymer-based nanostructures as...
Abstract Cyclic peptides are often used as scaffolds for the multivalent presentation of drug molecules due to their structural stability and constrained conformation. We identified a cyclic deca-peptide incorporating lipoamino acids delivering T helper B cell epitopes against group A Streptococcus (GAS), eliciting robust humoral immune responses. In this study, we assessed function-immunogenicity relationship multi-component vaccine candidate (referred VC-13) elucidate mechanism action....
The MATRIX protocol presents a novel miniaturized 24-well plate approach for microbial cultivation profiling, enabling the efficient and parallel assessment of metabolite production. This method integrates media within microbioreactor to facilitate creation diverse growth conditions cultivation. format supports simultaneous study multiple strains conditions, allowing discovery bioactive compounds. By employing variety compositions cultivations, enhances understanding metabolic pathways...
Immune stimulants (adjuvants) are essential vaccine components; however, clinically approved adjuvants limited with the majority being derived from pathogenic components. In this study, adjuvanting capacity of cholesterol, a natural human lipid, was explored following conjugation peptide antigens. A structure-activity relationship study conducted to compare linear and branched cholesterol conjugates other lipopeptide vaccines commercial adjuvants. Group Streptococcus (GAS) M protein-derived...