A5029816112- Autophagy in Disease and Therapy
- Lipid Membrane Structure and Behavior
- Ubiquitin and proteasome pathways
- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Neurogenetic and Muscular Disorders Research
- Cellular transport and secretion
- Genetics and Neurodevelopmental Disorders
- Biotin and Related Studies
- RNA Research and Splicing
- Amyotrophic Lateral Sclerosis Research
- Neuroscience and Neuropharmacology Research
- DNA Repair Mechanisms
- Protein Degradation and Inhibitors
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
The University of Queensland
2022-2024
The University of Melbourne
2014
Murdoch Children's Research Institute
2011-2013
Abstract To maintain both mitochondrial quality and quantity, cells selectively remove damaged or excessive mitochondria through mitophagy, which is a specialised form of autophagy. Mitophagy induced in response to diverse conditions, including hypoxia, cellular differentiation damage. However, the mechanisms that govern removal specific dysfunctional under steady‐state conditions fine‐tune content are not well understood. Here, we report SCF FBXL4 , an SKP1/CUL1/F‐box protein ubiquitin...
Friedreich ataxia (FA) is the most common and results from an expanded GAA repeat in first intron of FXN. This leads to epigenetic modifications reduced frataxin. We investigated relationships between genetic, epigenetic, clinical parameters a large case-control study FA.Clinical data samples were obtained individuals with FA during annual visits our dedicated clinic. expansions evaluated by polymerase chain reaction (PCR) restriction endonuclease digest. DNA methylation was measured using...
Primary liver cancer is the sixth most prevalent globally and often diagnosed late, when treatment options are limited. Secondary cancer, arising from metastasis of other cancers to liver, a common complication advanced solid significant cause cancer-related morbidity mortality. Existing for primary secondary tumours have limited efficacy new modalities potential improve patient outcomes. mRNA therapeutics readily delivered healthy after systemic administration, but their uptake expression...
In order to demonstrate the cell-surface localization of a putative transmembrane receptor in cultured neurons, we labeled protein on surface live neurons with specific primary antibody raised against an extracellular portion protein. Given that receptors are trafficked and from surface, if cells permeabilized after fixation then both internal will be detected by same secondary antibody. Here, adapted method used study trafficking ("antibody feeding") differentially label had been...
In order to demonstrate the cell-surface localization of a putative transmembrane receptor in cultured neurons, we labeled protein on surface live neurons with specific primary antibody raised against an extracellular portion protein. Given that receptors are trafficked and from surface, if cells permeabilized after fixation then both internal will be detected by same secondary antibody. Here, adapted method used study trafficking ("antibody feeding") differentially label had been...
Abstract Cells selectively remove damaged or excessive mitochondria through mitophagy, a specialized form of autophagy, to maintain mitochondrial quality and quantity. Mitophagy is induced in response diverse conditions, including hypoxia, cellular differentiation, damage. However, the mechanisms by which cells specific dysfunctional under steady-state conditions fine-tune content are not well understood. Here, we report that SCF FBXL4 , an SKP1/CUL1/F-box protein ubiquitin ligase complex,...
Abstract Genome-wide association studies (GWAS) have identified numerous candidate ALS risk variants, but their cellular functions are often unknown. Recent a variant of GGNBP2 that results in increased expression. To better understand how this gene might contribute to disease, we investigated the function Drosophila Ggnbp2 (dGgnbp2) motor neurons. Loss showed dGgnbp2 is required for neuron synaptic development. A human transgene completely rescued these phenotypes indicating functionally...