A5089635359- Plant Virus Research Studies
- Research in Cotton Cultivation
- Transplantation: Methods and Outcomes
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Insect Resistance and Genetics
- Extracellular vesicles in disease
- Genomics and Phylogenetic Studies
- Cancer-related molecular mechanisms research
- Plant Pathogenic Bacteria Studies
- Insect-Plant Interactions and Control
- Piperaceae Chemical and Biological Studies
- Silk-based biomaterials and applications
- Textile materials and evaluations
- Plant Molecular Biology Research
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Genetic and Environmental Crop Studies
- Plant and Fungal Interactions Research
- Metabolism and Genetic Disorders
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Plant tissue culture and regeneration
Huazhong Agricultural University
2007-2025
Wuxi People's Hospital
2025
Nanjing Medical University
2025
Yangzhou University
2023-2024
Shenzhen University
2024
University of Pittsburgh
2024
University of Pittsburgh Medical Center
2023
Washington University in St. Louis
2014-2022
Spice and Beverage Research Institute
2022
Chinese Academy of Tropical Agricultural Sciences
2022
Abstract Black pepper ( Piper nigrum ), dubbed the ‘King of Spices’ and ‘Black Gold’, is one most widely used spices. Here, we present its reference genome assembly by integrating PacBio, 10x Chromium, BioNano DLS optical mapping, Hi-C mapping technologies. The 761.2 Mb sequences (45 scaffolds with an N50 29.8 Mb) are assembled into 26 pseudochromosomes. A phylogenomic analysis representative plant genomes places magnoliids as sister to monocots-eudicots clade indicates that black has...
Abstract Background Millennia of directional human selection has reshaped the genomic architecture cultivated cotton relative to wild counterparts, but we have limited understanding selective retention and fractionation components. Results We construct a comprehensive variome based on 1961 cottons identify 456 Mb 357 sequence with domestication improvement signals 162 loci, 84 which are novel, including 47 loci associated 16 agronomic traits. Using pan-genome analyses, 32,569 8851...
Summary Cotton fibre is a unicellular seed trichome, and lint initials per as factor determines yield. However, the mechanisms controlling initiation from ovule epidermis are not understood well enough. Here, with single‐cell RNA sequencing (scRNA‐seq), total of 14 535 cells were identified cotton outer integument Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis day anthesis). Three major cell types, fibre, non‐fibre pigment layer then verified by in situ...
CRISPR/Cas-derived base editor enables precise editing of target sites and has been widely used for basic research crop genetic improvement. However, the efficiency editors at different targets varies greatly.
Summary The base‐editing technique using CRISPR / nC as9 (Cas9 nickase) or dC (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, novel G. hirsutum ‐Base Editor 3 (Gh BE 3) system has been developed single‐base mutations in the allotetraploid genome of cotton ( Gossypium ). A sequence APOBEC ) and uracil glycosylase inhibitor UGI was inserted into our /Cas9 plasmid pRGEB 32‐GhU6.7). Three target sites were chosen for two genes, Gh CLA...
The immunological role of exosomes in allograft rejection remains unknown. We sought to determine whether are induced during lung and define the antigenic compositions HLA, lung-associated self-antigens (SAgs) microRNAs (miRNAs). Exosomes were isolated from sera bronchoalveolar lavage fluid 30 transplant recipients (LTxRs) who stable or had acute (AR) bronchiolitis obliterans syndrome (BOS). defined by flow cytometry for CD63 western blotting annexin V SAgs, collagen (Col-V) Kα1 tubulin...
Wintersweet (Chimonanthus praecox), an important ornamental plant, has evolved unique fragrant aroma and winter-flowering properties, which are critical for its successful sexual reproduction. However, the molecular mechanisms underlying these traits largely unknown in this species. In addition, wintersweet is also a typical representative species of magnoliids, where phylogenetic position relative to eudicots monocots not been conclusively resolved.Here, we present chromosome-level genome...
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with single nuclease domain, relatively small size, low‐frequency off‐target effects cleavage capability under high temperature has recently established designated CRISPR/Cas12b (C2c1). Cas12b also shown inducibility in mammalian systems. Therefore, this system is potentially valuable editing the genomes of plant species, such as cotton, that are resistant to temperatures. Using...
Abstract Transposable element (TE) amplification has been recognized as a driving force mediating genome size expansion and evolution, but the consequences for shaping 3D genomic architecture remains largely unknown in plants. Here, we report reference-grade assemblies three species of cotton ranging 3-fold size, namely Gossypium rotundifolium (K2), G. arboreum (A2), raimondii (D5), using Oxford Nanopore Technologies. Comparative analyses document details lineage-specific TE contributing to...
Abstract Background Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation undesirable double-stranded breaks (DSB). Studies single-nucleotide polymorphisms (SNPs) underpinning traits are still challenging, particularly polyploidy SNPs present multiple copies, simultaneous modification all alleles would be...
Summary Artemisia argyi , as famous annua is a medicinal plant with huge economic value in the genus of and has been widely used world for about 3000 years. However, lack reference genome severely hinders understanding genetic basis active ingredient synthesis A. . Here, we firstly report complex chromosome‐level assembly large size 8.03 Gb, features high heterozygosity (2.36%), repetitive sequences (73.59%) number protein‐coding genes (279 294 total). The reveals at least three rounds...
Abstract Background Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible reprogramming during somatic are still largely unclear. Recent advances in single-cell technologies enable us to explore mechanism of regeneration at resolution. Results We generate high-resolution transcriptomic landscape hypocotyl tissue from highly regenerable cotton genotype Jin668 recalcitrant TM-1. identify nine putative clusters...
Abstract Insects pose significant challenges in cotton‐producing regions. Here, they describe a high‐throughput CRISPR/Cas9‐mediated large‐scale mutagenesis library targeting endogenous insect‐resistance‐related genes cotton. This targeted 502 previously identified using 968 sgRNAs, generated ≈2000 T0 plants and achieved 97.29% genome editing with efficient heredity, reaching upto 84.78%. Several potential resistance‐related mutants (10% of 200 lines) their that may contribute to...
Summary Oil‐Camellia ( Camellia oleifera ), belonging to the Theaceae family Camellia, is an important woody edible oil tree species. The in its mature seed kernels, mainly consists of more than 90% unsaturated fatty acids, tea polyphenols, flavonoids, squalene and other active substances, which one best quality vegetable oils world. However, genetic research molecular breeding on oil‐Camellia are challenging due complex background. Here, we successfully report a chromosome‐scale genome...
The efficiency and accuracy of the CRISPR/Mb2Cas12a system were demonstrated in cotton, achieving an over 90% at target sites. Notably, Mb2Cas12a exhibited significant tolerance under different temperatures ranging from 22°C to 32°C. Additionally, revealed effective editing more relaxed VTTV PAM sites cotton genome, which expanded genome range by approximately 2.6-fold than wide-type LbCas12a. Finally, a multiplex was also developed based on Mb2Cas12a, enabling simultaneous eight using...
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) temperature-sensitive system for plant genome editing (Malzahn et al., 2019) has been successfully applied in such as rice, soya bean, tobacco, maize cotton (Lee 2019; Li 2018; Tang 2017; Xu 2019). However, the temperature sensitivity of LbCpf1 not tested yet, high temperature-resistant crop. In order improve efficiency...
Panax notoginseng (2n = 2x 24), reputed as a valuable medicinal plant, belongs to the Araliaceae family (Figure 1a). P. has been used traditional Chinese medicine with obvious efficacy and favourable safety. Currently, is an important material of many well-known patent medicines including Xuesaitong, Yunnan Baiyao Compound Danshen Dripping Pills (Xu et al., 2019). Triterpenoid saponins (TSs) dencichine are main bioactive compounds in notoginseng. The therapeutic effects TSs include...
As the basic building blocks of all living species, cells play a crucial role in sustaining life activities. Traditional bulk methods only give us molecular insight into tissue, organ, or/and individual. With rapid development sequencing technology, especially 10× Genomics based on microfluidic omics, biological research has entered era single-cell level, which enables to gain activities from more microscopic perspective. this cutting-edge it is now possible mine heterogeneity between tissue...
Zanthoxylum armatum and bungeanum, known as 'Chinese pepper', are distinguished by their extraordinary complex genomes, phenotypic innovation of adaptive evolution species-special metabolites. Here, we report reference-grade genomes Z. bungeanum. Using high coverage sequence data comprehensive assembly strategies, derived 66 pseudochromosomes comprising 33 homologous phased groups two subgenomes, including autotetraploid armatum. The genomic rearrangements whole-genome duplications created...
Two polar organic–inorganic metal halides (C 20 H P)CuX 2 (X = Cl, Br) feature rare quasi-linear [CuX ] − units and exhibit phase-matchable SHG effects, the structure–NLO properties relationship is investigated systematically.
Calcium-dependent protein kinases (CDPKs) act as key signal transduction enzymes in plants, especially response to diverse stresses, including herbivory. In this study, a comprehensive analysis of the CDPK gene family upland cotton revealed that GhCPKs are widely expressed multiple tissues and respond positively various biotic abiotic stresses. We developed strategy for screening insect-resistance genes from CRISPR-Cas9 mutant library GhCPKs. The was created using 246 single-guide RNAs...
Abstract Background Heat-shock transcription factor 4 (HSF4) mutations are associated with autosomal dominant lamellar cataract and Marner cataract. Disruptions of the Hsf4 gene cause lens defects in mice, indicating a requirement for HSF4 fiber cell differentiation during development. However, neither relationship between crystallins nor detailed mechanism maintenance transparency by is fully understood. Results In an attempt to determine how underlying biomedical physiological mechanisms...
Plant bugs (Miridae species), which are sap-sucking insects, have emerged as major pests of cotton in China. Most Miridae species not sensitive to commercial Bacillus thuringiensis (Bt) cotton, resulting significant economic losses and an increased application insecticide, eventually may compromise the future Bt cotton. We demonstrate that FATTY ACYL-COA REDUCTASE (AsFAR) plays essential role reproduction bug Adelphocoris suturalis. Down-regulation AsFAR expression by injection...