A5101476581- Retinoids in leukemia and cellular processes
- interferon and immune responses
- Acute Myeloid Leukemia Research
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Fibroblast Growth Factor Research
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Receptor Mechanisms and Signaling
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Genetics and Reproduction
- Protein Degradation and Inhibitors
- Liver physiology and pathology
- Adipose Tissue and Metabolism
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
Shanghai Jiao Tong University
2015-2025
Shanghai Institute of Hematology
2006-2025
Shanghai Model Organisms (China)
2014-2024
Ruijin Hospital
2013-2024
State Key Laboratory of Medical Genomics
2005-2024
Deleted Institution
2011-2019
Tongji University
2012-2019
Army Medical University
2016
Daping Hospital
2016
Chinese Academy of Sciences
2006-2015
Two reports from China have suggested that arsenic trioxide can induce complete remissions in patients with acute promyelocytic leukemia (APL). We evaluated this drug APL an attempt to elucidate its mechanism of action.
Abstract Upon recognition of viral components by pattern receptors, including TLRs and retinoic acid-inducible gene I (RIG-I)- like helicases, cells are activated to produce type IFN proinflammatory cytokines. These pathways tightly regulated host prevent inappropriate cellular response, but viruses can down-regulate these for their survival. Recently, identification negative regulators cytoplasmic RNA-mediated antiviral signaling, especially the RIG-I pathway, attract much attention....
Abstract Effective recognition of viral infection and subsequent triggering antiviral innate immune responses are essential for the host defense, which is tightly regulated by multiple regulators, including microRNAs. Our previous study showed that a panel microRNAs, miR-155, was markedly upregulated in macrophages upon vesicular stomatitis virus infection; however, biological function miR-155 during remains unknown. In this paper, we show RNA induces expression via TLR/MyD88-independent but...
MicroRNAs (miRNAs) mainly function as post-transcriptional regulators and are involved in a wide range of physiological pathophysiological processes such cell proliferation, differentiation, apoptosis, tumorigenesis. Mouse testes express large number miRNAs. However, the roles these testicular miRNAs remain largely unknown. Using microarray quantitative real time PCR assays, we identified that microRNA-449 (miR-449) cluster were preferentially expressed mouse testis, their levels drastically...
There are currently two distinct models proposed to explain why both MDM2 and MDMX required in p53 control, with a key difference centered on whether these inhibitors work together or independently. To test competing models, we generated knockin mice expressing point mutation mutant (C462A) that is defective binding. This approach allowed targeted disassociation of the MDM2/MDMX heterocomplex without affecting ability bind p53, while leaving protein itself completely untouched....
Abstract Mas-related G protein-coupled receptors (Mrgs) are a subset of GPCRs linked to pain modulation. MrgF was identified as an orphan Mrg whose function and ligand remain unclear. In this study, in addition its expression the dorsal root ganglia (DRG), primary afferent center that transmits pain, we dense , particularly concentrated Purkinje cell layer mouse cerebellum. Given important role neurons both motor modulation non-motor modulation, including processing, established knockout (...
Acute promyelocytic leukemia (APL) is associated with chromosomal translocations always involving the RARalpha gene, which variably fuses to one of several distinct loci, including PML or PLZF (X genes) in t(15;17) t(11;17), respectively. APL patients harboring responds well retinoic acid (RA) treatment and chemotherapy, whereas t(11;17) poorly both treatments, thus defining a syndrome. Here, we show that RA, As(2)O(3), RA + As(2)O(3) prolonged survival either leukemic PML-RARalpha...
The promyelocytic leukemia (PML) gene encodes a putative tumor suppressor involved in the control of apoptosis, which is fused to retinoic acid receptor α (RARα) vast majority acute (APL) patients as consequence chromosomal translocations. PMLRARα oncoprotein thought antagonize function PML through its ability heterodimerize with and delocalize from nuclear body. In APL, this may be facilitated by reduction heterozygosity normal allele. To determine whether acts vivo what consequences...
RA-inducible gene I (RIG-I/DDX58) has been shown to activate IFN-β promoter stimulator 1 (IPS-1) on recognizing cytoplasmic viral RNAs. It is unclear how RIG-I functions within the IFN and/or RA signaling process in acute myeloid leukemia (AML) cells, however, where obvious induction observed. Here, we show that functionally contributes IFN-α plus RA-triggered growth inhibition of AML cells. Interestingly, although itself under regulation STAT1, a major intracellular signal mediator,...
In order to understand the mechanisms of mammalian fertilization, studies using genetically manipulated animals have provided us with plenty interesting and valuable information on genetic factors affecting male fertility. present work, we demonstrate for first time that Prss37, a previously uncharacterized putative trypsin-like serine protease, is required Prss37 highly exclusively expressed in testis adult mice, especially elongating spermatids during spermiogenesis, almost vanishes mature...
Fibroblast growth factor 9 (Fgf9) is a well-known that regulates bone development; however, its function in homeostasis still unknown. Previously, we identified point mutation the FGF9 gene (p.Ser99Asn, S99N) and generated an isogeneic knock-in mouse model, which revealed this loss-of-function impaired early joint formation was responsible for human multiple synostosis syndrome 3 (SYNS3). Moreover, newborn adult S99N mutant mice exhibited significantly increased mass, suggesting Fgf9 also...
Abstract Background Heat-shock transcription factor 4 (HSF4) mutations are associated with autosomal dominant lamellar cataract and Marner cataract. Disruptions of the Hsf4 gene cause lens defects in mice, indicating a requirement for HSF4 fiber cell differentiation during development. However, neither relationship between crystallins nor detailed mechanism maintenance transparency by is fully understood. Results In an attempt to determine how underlying biomedical physiological mechanisms...
Retinoic acid inducible gene I (RIG-I) senses viral RNAs and triggers innate antiviral responses through induction of type IFNs inflammatory cytokines. However, whether RIG-I interacts with host cellular RNA remains undetermined. Here we report that Rig-I multiple mRNAs, especially Nf-κb1. is required for NF-κB activity via regulating Nf-κb1 expression at posttranscriptional levels. It the binding sites within 3'-UTR mRNA. Further analyses reveal three distinct tandem motifs enriched in...
Maintaining the functional integrity of mitochondria is crucial for cell function, signal transduction and overall activities. Mitochondrial dysfunctions may alter energy metabolism in many cases are associated with neurological diseases. Recent studies have reported that mutations dehydrogenase E1 transketolase domain‐containing 1 ( DHTKD1 ), a mitochondrial protein encoding gene, could cause abnormalities. However, function remains unknown. Here, we report strong correlation expression...
Tssk4 belongs to the Testis Specific Serine/threonine protein Kinase (TSSK) family, members of which play an important role in spermatogenesis and/or spermiogenesis. Several Tssk family proteins have extensively been studied. However, exact function remains unclear. A knockout mouse model was generated and males were subfertile due seriously decreased sperm motility. The ultrastructure Tssk4(-/-)sperm tail is disorganized at midpiece-principal piece junction, leading a severe bend flagellum....
Testis-specific PRSS55 is a highly conserved chymotrypsin-like serine protease among mammalian species. So far, the physiological function of remains unknown. Here, we show that GPI-anchored membrane protein, specifically expressed in adult mouse testis and mainly observed luminal side seminiferous tubules sperm acrosome. Mice deficient for Prss55 develop male infertile with normal reproduction-related parameters observed. Interestingly, vivo fertilization rate Prss55−/− males dramatically...
Retinoic acid-inducible gene-I (Rig-I) is an intracellular viral RNA receptor, which specifically recognizes double-stranded initiating antiviral innate immunity. Increasing evidences showed that Rig-I had broader roles in antibacterial immunity and cancer protection. However, the potential mechanisms of gut flora regulation colorectal (CRC) progression remain unclear.Immunohistochemistry was performed to detect protein 38 pairs CRC tissue matched adjacent mucosa, immunofluorescence western...