A5000979495- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Endoplasmic Reticulum Stress and Disease
- Invertebrate Immune Response Mechanisms
- Ubiquitin and proteasome pathways
- Sperm and Testicular Function
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- Microtubule and mitosis dynamics
- Heat shock proteins research
- Hippo pathway signaling and YAP/TAZ
- Insect behavior and control techniques
- Cellular transport and secretion
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- Antenna Design and Analysis
- Autophagy in Disease and Therapy
- Advanced Antenna and Metasurface Technologies
- RNA modifications and cancer
- Lipid metabolism and biosynthesis
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Insect Utilization and Effects
Yangzhou University
2015-2023
Fudan University
2005-2023
State Key Laboratory of Genetic Engineering
2001-2020
National Institutes of Health
2014-2019
Health and Human Development (2HD) Research Network
2018-2019
National Changhua University of Education
2017-2019
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2014-2019
Zhongshan Hospital
2018
Shanghai Institutes for Biological Sciences
2009-2012
Shanghai Jiao Tong University
2009-2012
Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus and one of the most common agents viral encephalitis. The infectious entry process JEV into host cells remains largely unknown. Here, we present systemic study concerning cellular mechanism to B104 rat neuroblastoma cells. It was observed that internalization inhibited by chloroquine ammonium chloride, both which can elevate pH acidic organelles. However, not affected chlorpromazine, overexpression dominant-negative form EPS 15...
Abstract Aflatoxin B1 (AFB1) is a potent hepatocarcinogen in humans and exposure to AFB1 known cause both acute chronic hepatocellular injury. As the liver be main target organ of aflatoxin, it important identify key molecules that participate AFB1-induced hepatotoxicity investigate their underlying mechanisms. In this study, critical role caveolin-1 hepatic cell apoptosis was examined. We found decrease viability an increase oxidation human hepatocyte L02 cells after exposure. addition,...
Significance The target of rapamycin complex 1 (TORC1) promotes cell growth and anabolic metabolism. In yeast, entry into meiosis is contingent on the down-regulation TORC1 activity by increased minichromosome loss 1/GTPase-activating proteins toward Rags (Iml1/GATOR1) in response to amino acid starvation. Here we define developmental requirements for regulators Iml1/GATOR1 GATOR2 during Drosophila oogenesis. We demonstrate that, as observed down-regulates facilitate mitotic/meiotic...
Tssk4 belongs to the Testis Specific Serine/threonine protein Kinase (TSSK) family, members of which play an important role in spermatogenesis and/or spermiogenesis. Several Tssk family proteins have extensively been studied. However, exact function remains unclear. A knockout mouse model was generated and males were subfertile due seriously decreased sperm motility. The ultrastructure Tssk4(-/-)sperm tail is disorganized at midpiece-principal piece junction, leading a severe bend flagellum....
A design for antennas with quad-polarization diversity is described. The antenna structure mainly composed of a reconfigurable crossed-dipole fed coaxial baluns and switchable feeding network. By controlling the states p-i-n diodes embedded in balun feeds, crossed dipole can generate vertical horizontal polarization radiations, phase each mode be switched between 0° 180°. As network, it has four output ports only two are set to active excite at time. integrating resultant provide modes,...
TORC1 is a master regulator of metabolism in eukaryotes that responds to multiple upstream signaling pathways. The GATOR complex newly defined contains two sub-complexes, GATOR1, which inhibits activity response amino acid starvation and GATOR2, opposes the GATOR1. While GATOR1 has been implicated wide array human pathologies including cancer hereditary forms epilepsy, vivo relevance GATOR2 remains poorly understood metazoans. Here we define role component Wdr24 Drosophila. Using combination...
Abstract Acute lung injury (ALI) is a severe respiratory disease with high mortality rate. The integrity of the pulmonary endothelial barrier influences development and prognosis ALI. Therefore, it has become an important target for ALI treatment. Extracellular vesicles (EVs) are promising nanotherapeutic agents against Herein, endothelium‐derived engineered extracellular (eEVs) that deliver microRNA‐125b‐5p (miRNA‐125b) to tissues exerting protective effect on reported. eEVs modified...
Aim: To investigate the detailed mechanism of Japanese encephalitis virus (JEV) cell entry. Materials & methods: Utilize a siRNA library targeting cellular membrane trafficking genes to identify key molecules that mediate JEV entry into human neuronal cells. Results: enters cells by caveolin-1-mediated endocytosis, which depends on two-step regulation actin cytoskeleton remodeling triggered RhoA and Rac1: activation promoted phosphorylation caveolin-1, then Rac1 facilitated...
// Pingzhao Zhang 1,2,* , Yuanyuan 1,* Kun Gao 1 Yuqi Wang Xiaofeng Jin Youheng Wei Heige Saiyin Dejie 3 Jintao Peng 4 Jian Ma Yan Tang Reziya Wumaier Hongxiu Yu 2 Yimin Dong 5 Haojie Huang 6 Long and Chenji State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics Development, School Life Sciences, Fudan University, Shanghai, P.R. China Institutes Biomedical P. R. Department Gastroenterology, Jiangxi Institute Gastroenterology & Hepatology, The First...
PARP12 is a mono-ADP-ribosyltransferase, but its function remains largely unknown. Here, we identified four-and-a-half LIM-only protein 2 (FHL2) as functional partner of through affinity purification. Although did not mono-ADP-ribosylate FHL2 in vitro and vivo, deficiency decreased the level by promoting ubiquitination increased expression transforming growth factor beta1 (TGF-β1), which independent enzymatic activity. We also provided evidence that migration invasion hepatocellular...
Pancreatic ductal adenocarcinoma (PDAC) is a nearly lethal neoplasm. It remarkably stroma-rich, vascular-poor and hypo-perfused tumour, which prevents efficient drug delivery. Paradoxically, the neoplastic cells have robust glucose uptake, suggesting that microvasculature has adopted an alternative method for nutrient uptake cellular trafficking. Using adapted thick tumour section immunostaining three-dimensional (3D) construction imaging in human tissue samples, we identified undiscovered...
Abstract TORC1 regulates metabolism and growth in response to a large array of upstream inputs. The evolutionarily conserved trimeric GATOR1 complex inhibits activity amino acid limitation. In humans, the has been implicated wide pathologies including cancer hereditary forms epilepsy. However, precise role animal physiology remains largely undefined. Here, we characterize null mutants components nprl2, nprl3, iml1 Drosophila melanogaster. We demonstrate that all three have inappropriately...
Nitric oxide (NO) at a high concentration is an effector to kill pathogens during insect immune responses, it also functions as second messenger low regulate antimicrobial peptide (AMP) production in insects. Drosophila calcineurin subunit CanA1 ubiquitous serine/threonine protein phosphatase involved NO-induced AMP production. However, unclear how NO regulates expression. In this study, we used lepidopteran pest Ostrinia furnacalis and S2 cells investigate signaling affects the Bacterial...
As a member of the testis-specific serine/threonine protein kinase (TSSK) family, Tssk4 is exclusively expressed in testis and plays an essential role male fertility. We previously reported that can associate with phosphorylate Odf2, but phosphorylation site still unknown. Here we confirm C-terminal region (amino acids 214-638) Odf2 required for association Tssk4. Furthermore, to identify at which phosphorylates generated several point mutants (Ser/Thr/Lys Ala) identified serine 76 as one...
Progestins has been used widely for endometrial cancer (EC) patients. However, long term use of high dose progestin often lead to resistance. Our previous studies have demonstrated that metformin reversed resistance through the downregulation expression glyoxalase I (GLOI) in type cancer. Recent role Ten-eleven translocation 1 (TET1) cancer, but physiological TET1 GLOI-mediated poorly addressed. Immunohistochemistry was detect TET1, GLOI and 5hmC various endometrium. Western blot carried out...
The TORC1 regulator GATOR1/SEACIT controls meiotic entry and early events in yeast. However, how metabolic pathways influence progression metazoans remains poorly understood. Here we examine the role of regulators GATOR1 GATOR2 response to double-stranded breaks (DSB) during
RPK118 is a sphingosine kinase-1-binding protein that has been implicated in 1 phosphate-mediated signaling. It contains PX (phox homology) domain and two pseudo-kinase domains, co-localizes with kinase-1 on early endosomes. In this study we identified novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed pull-down assays co-immunoprecipitation experiments. Deletion studies showed interacted through its...