A5101991101- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Research in Cotton Cultivation
- Insect Resistance and Genetics
- Insect-Plant Interactions and Control
- Geomagnetism and Paleomagnetism Studies
- Geology and Paleoclimatology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant and Fungal Interactions Research
- Immune cells in cancer
- Geological formations and processes
- Insect symbiosis and bacterial influences
- Cancer-related molecular mechanisms research
- Plant Molecular Biology Research
- RNA Research and Splicing
- Lipid metabolism and disorders
- Insect and Pesticide Research
- Advanced biosensing and bioanalysis techniques
- Biochemical and biochemical processes
- Cell Image Analysis Techniques
- Plant Pathogenic Bacteria Studies
- Electrospun Nanofibers in Biomedical Applications
- RNA regulation and disease
- Circular RNAs in diseases
- Plant Gene Expression Analysis
Huazhong Agricultural University
2019-2025
Henan Agricultural University
2024
Northwest University
2023-2024
Southern Medical University
2023
Nanfang Hospital
2023
Guangzhou Experimental Station
2023
Wenzhou Medical University
2021
First Affiliated Hospital of Wenzhou Medical University
2021
Zero to Three
2020
Nanjing Medical University
2019
Abstract Background Millennia of directional human selection has reshaped the genomic architecture cultivated cotton relative to wild counterparts, but we have limited understanding selective retention and fractionation components. Results We construct a comprehensive variome based on 1961 cottons identify 456 Mb 357 sequence with domestication improvement signals 162 loci, 84 which are novel, including 47 loci associated 16 agronomic traits. Using pan-genome analyses, 32,569 8851...
Summary The base‐editing technique using CRISPR / nC as9 (Cas9 nickase) or dC (deactivated Cas9) fused with cytidine deaminase is a powerful tool to create point mutations. In this study, novel G. hirsutum ‐Base Editor 3 (Gh BE 3) system has been developed single‐base mutations in the allotetraploid genome of cotton ( Gossypium ). A sequence APOBEC ) and uracil glycosylase inhibitor UGI was inserted into our /Cas9 plasmid pRGEB 32‐GhU6.7). Three target sites were chosen for two genes, Gh CLA...
Summary The CRISPR/Cas9 and Cas12a (Cpf1) tools have been used on a large scale for genome editing. A new effector with single nuclease domain, relatively small size, low‐frequency off‐target effects cleavage capability under high temperature has recently established designated CRISPR/Cas12b (C2c1). Cas12b also shown inducibility in mammalian systems. Therefore, this system is potentially valuable editing the genomes of plant species, such as cotton, that are resistant to temperatures. Using...
Abstract Background Base editors (BEs) display diverse applications in a variety of plant species such as Arabidopsis, rice, wheat, maize, soybean, and cotton, where they have been used to mediate precise base pair conversions without the collateral generation undesirable double-stranded breaks (DSB). Studies single-nucleotide polymorphisms (SNPs) underpinning traits are still challenging, particularly polyploidy SNPs present multiple copies, simultaneous modification all alleles would be...
Abstract Background The pathogenesis of Parkinson's disease (PD) has not been fully elucidated, and there are no effective disease-modifying drugs for the treatment PD. Mesenchymal stem cells have used to treat several diseases, but readily available. Methods Here, we phenotypically uniform trophoblast stage-derived mesenchymal (T-MSCs) from embryonic cells, which capable stable production, their exosomes (T-MSCs-Exo) explore molecular mechanisms involved in dopaminergic (DA) neuron...
Abstract Insects pose significant challenges in cotton‐producing regions. Here, they describe a high‐throughput CRISPR/Cas9‐mediated large‐scale mutagenesis library targeting endogenous insect‐resistance‐related genes cotton. This targeted 502 previously identified using 968 sgRNAs, generated ≈2000 T0 plants and achieved 97.29% genome editing with efficient heredity, reaching upto 84.78%. Several potential resistance‐related mutants (10% of 200 lines) their that may contribute to...
Lignins and their antimicrobial-related polymers cooperatively enhance plant resistance to pathogens. Several isoforms of 4-coumarate-coenzyme A ligases (4CLs) have been identified as indispensable enzymes involved in lignin flavonoid biosynthetic pathways. However, roles plant-pathogen interaction are still poorly understood. This study uncovers the role Gh4CL3 cotton vascular pathogen Verticillium dahliae. The 4CL3-CRISPR/Cas9 mutant (CR4cl) exhibited high susceptibility V. was most...
Cotton (Gossypium hirsutum) is an allotetraploid species and a typical thermophilic crop that can survive grow well under temperatures up to 45 °C. CRISPR/LbCpf1 (LbCas12a) temperature-sensitive system for plant genome editing (Malzahn et al., 2019) has been successfully applied in such as rice, soya bean, tobacco, maize cotton (Lee 2019; Li 2018; Tang 2017; Xu 2019). However, the temperature sensitivity of LbCpf1 not tested yet, high temperature-resistant crop. In order improve efficiency...
As the basic building blocks of all living species, cells play a crucial role in sustaining life activities. Traditional bulk methods only give us molecular insight into tissue, organ, or/and individual. With rapid development sequencing technology, especially 10× Genomics based on microfluidic omics, biological research has entered era single-cell level, which enables to gain activities from more microscopic perspective. this cutting-edge it is now possible mine heterogeneity between tissue...
Calcium-dependent protein kinases (CDPKs) act as key signal transduction enzymes in plants, especially response to diverse stresses, including herbivory. In this study, a comprehensive analysis of the CDPK gene family upland cotton revealed that GhCPKs are widely expressed multiple tissues and respond positively various biotic abiotic stresses. We developed strategy for screening insect-resistance genes from CRISPR-Cas9 mutant library GhCPKs. The was created using 246 single-guide RNAs...
Sap-sucking insects cause severe damage to cotton production. Long non-coding RNAs (lncRNAs) play vital regulatory roles in various development processes and stress response, however, the function of lncRNAs during sap-sucking insect infection is largely unknown. In this study, transcriptome profiles between resistant (HR) susceptible (ZS) cultivars under whitefly infestation at different time points (0, 4, 12, 24, 48 h) were compared. A total 6,651 transcript 606 differentially expressed...
Cancer metastatic organotropism is still a mystery. The liver known to be susceptible cancer metastasis and alcoholic injury. However, it unclear whether how alcohol facilitates intervene. Here, we show that preferentially promotes in colon-cancer-bearing mice post-surgery pancreatic patients. mechanism triggers an extra- intrahepatic crosstalk reshape immunosuppressive microenvironment. In detail, upregulates extrahepatic IL-6 hepatocellular receptor expression, resulting hepatocyte STAT3...
Abstract Key message The transcriptomic, phenotypic and metabolomic analysis of transgenic plants overexpressing GhMPK31 in upland cotton revealed the regulation H 2 O burst synthesis defensive metabolites by . Mitogen-activated protein kinases (MAPKs) are a crucial class kinases, which play an essential role various biological processes plants. Upland ( G. hirsutum ) is most widely cultivated species with high economic value. To gain better understanding MAPK gene family, we conducted...
Abstract N 6 ‐methyladenosine (m A) is the most prevalent internal modification of mRNA and plays an important role in regulating plant growth. However, there still a lack effective tools to precisely modify m A sites individual transcripts plants. Here, programmable editing are developed by combining CRISPR/dCas13(Rx) with methyltransferase GhMTA ( T argeted RNA M ethylation E ditor, TME ) or demethyltransferase GhALKBH10 D emethylation TDE ). These editors enable efficient deposition...
Upland cotton (Gossypium hirsutum), an allotetraploid, contains At- and Dt- subgenome most genes have multiple homologous copies, which pose a huge challenge to investigate genes' function due the functional redundancy. Therefore, it is of great significance establish effective techniques for genomics in cotton. In this study, we tested two novel genome editing vectors compared them with CRISPR/Cas9 system (pRGEB32-GhU6.7) developed our laboratory previously. first new vector, sgRNA...
Cotton (Gossypium hirsutum) is an important fiber crop worldwide. Insect attack causes cotton yield and quality losses. However, little known about the mechanism of response to insect attack. We simulated feeding by applying oral secretions (OS) wounds, combined transcriptome metabolome analysis investigate how OS from two major pest species (Helicoverpa armigera Spodoptera litura) affect defense responses. found that respectively 12,668 13,379 genes were differentially expressed in...
Although the regulatory function of miRNAs and their targets have been characterized in model plants, a possible underlying role cotton response to herbivore infestation has not determined. To investigate this, we performed small RNA degradome sequencing between resistant susceptible cultivar following with generalist whitefly. In total, 260 miRNA families 241 were identified. Quantitative-PCR analysis revealed that several corresponding exhibited dynamic spatio-temporal expression patterns....
Radiation therapy is an effective method in the management of esophageal cancer. MicroRNAs (miRNAs) have been reported to play important role tumorigenesis. However, roles specific miRNAs radioresistant cancer remain be investigated. In present study, relative expression level miR-20b-5p and miR-125a-5p were evaluated by quantitative Real-time polymerase chain reaction. Cell counting Kit-8 assay, wound-healing transwell assay used assess cell proliferation, migration invasion. TUNEL Annexin...
Although the regulatory function of miRNAs and their targets have been characterized in model plants, a possible underlying role cotton response to herbivore infestation has not determined. To investigate this, we performed small RNA degradome sequencing between resistant susceptible cultivar following with generalist whitefly. In total, 260 miRNA families 241 were identified. Quantitative-PCR analysis revealed that several corresponding exhibited dynamic spatio-temporal expression patterns....
Key factors of pyroptosis play an important role in the inflammatory response to connective tissue disease (CTD). However, information on active and stable stages CTD is scarce. To distinguish differences concentrations C-reactive protein (CRP), caspase 1, 4, 5 sCD14 plasma between patients with CTD. A cohort study was conducted recruit diagnosed phase as well health control. These data included analysis concentration sCD14, 4 peripheral by ELISA. The Wilcoxon rank-sum test used compare two...
Lacustrine sediments are good recorders of palaeoenvironment changes and have been widely studied in recent years. The study lacustrine Southwest China will improve our understanding the complex environmental evolution history China. Therefore, this paper presents a high-resolution rock magnetism from Pengtun drilling hole (PT2) Heqing Basin, results show that dominant magnetic minerals PT2 drill core magnetite, maghemite haematite. susceptibility (χ) upper part (0–13.5 m) is lower than...