A5047598277- Research in Cotton Cultivation
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Gene Regulatory Network Analysis
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Silk-based biomaterials and applications
- Evolution and Genetic Dynamics
- Photosynthetic Processes and Mechanisms
- Seed Germination and Physiology
- stochastic dynamics and bifurcation
- RNA modifications and cancer
- Textile materials and evaluations
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Plant responses to water stress
- Single-cell and spatial transcriptomics
- Lipid metabolism and biosynthesis
- Insect-Plant Interactions and Control
- Transgenic Plants and Applications
- Rice Cultivation and Yield Improvement
Guangdong University of Finance
2015-2025
Huazhong Agricultural University
2016-2025
Henan University of Science and Technology
2024
Shanghai Zhangjiang Laboratory
2022-2024
Yangtze University
2023
Children's Hospital of Chongqing Medical University
2022
Chongqing Medical University
2022
Wuhan College
2019-2021
University of California, Berkeley
2018
Durham University
2013
Allotetraploid cotton species (Gossypium hirsutum and Gossypium barbadense) have long been cultivated worldwide for natural renewable textile fibers. The draft genome sequences of both are available but they highly fragmented incomplete1-4. Here we report reference-grade assemblies annotations G. accession Texas Marker-1 (TM-1) barbadense 3-79 by integrating single-molecule real-time sequencing, BioNano optical mapping high-throughput chromosome conformation capture techniques. Compared with...
Abstract Somatic embryogenesis is the developmental process by which somatic cells undergo restructuring to generate embryogenic cells. These then go through a series of morphological and biochemical changes that result in formation or non-zygotic embryo capable regenerating plants. represents unique pathway includes number characteristic events: dedifferentiation cells, activation cell division, reprogramming their physiology, metabolism, gene expression patterns. Valuable studies have...
Abstract Gossypium hirsutum contributes the most production of cotton fibre, but G. barbadense is valued for its better comprehensive resistance and superior fibre properties. However, allotetraploid genome has not been comprehensively analysed. Here we present a high-quality assembly 2.57 gigabase , including 80,876 protein-coding genes. The double-sized A (or At) (1.50 Gb) against D Dt) (853 Mb) primarily resulted from expansion Gypsy elements, Peabody Retrosat2 subclades in Del clade...
Abstract Male reproduction in flowering plants is highly sensitive to high temperature (HT). To investigate molecular mechanisms of the response cotton (Gossypium hirsutum) anthers HT, a relatively complete comparative transcriptome analysis was performed during anther development lines 84021 and H05 under normal HT conditions. In total, 4,599 differentially expressed genes were screened; mainly related epigenetic modifications, carbohydrate metabolism, plant hormone signaling. Detailed...
Plants are constantly challenged by a multitude of pathogens and pests, which causes massive yield quality losses annually. A promising approach to reduce such is enhance the immune system plants through genetic engineering. Previous work has shown that laccases (p-diphenol:dioxygen oxidoreductase, EC 1.10.3.2) function as lignin polymerization enzymes. Here we demonstrate transgenic manipulation expression laccase gene GhLac1 in cotton (Gossypium hirsutum) can confer an enhanced defense...
Summary Cotton fibre is a unicellular seed trichome, and lint initials per as factor determines yield. However, the mechanisms controlling initiation from ovule epidermis are not understood well enough. Here, with single‐cell RNA sequencing (scRNA‐seq), total of 14 535 cells were identified cotton outer integument Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis day anthesis). Three major cell types, fibre, non‐fibre pigment layer then verified by in situ...
CRISPR/Cas-derived base editor enables precise editing of target sites and has been widely used for basic research crop genetic improvement. However, the efficiency editors at different targets varies greatly.
MicroRNAs (miRNAs) are endogenous non-coding ~21 nucleotide RNAs that regulate gene expression at the transcriptional and post-transcriptional levels in plants animals. They play an important role development, abiotic stress, pathogen responses. miRNAs with their targets have been widely studied model plants, but limited knowledge is available on small RNA population of cotton (Gossypium hirsutum)-an economic crop, global identification related through degradome sequencing has not developed...
WRKY transcription factors are transcriptional regulators of signaling pathways involved in biotic and abiotic stress responses. In this study, we report that ectopic expression the GhWRKY6‐like gene significantly improved salt tolerance Arabidopsis thaliana while silencing increase sensitivity to stresses cotton. was localized nucleus. Expression remarkably induced by salt, polyethylene glycol (PEG) abscisic acid (ABA) treatments. For further characterization, cloned transformed into . Our...
Abstract Background Somatic embryogenesis (SE), by which somatic cells of higher plants can dedifferentiate and reorganize into new plants, is a notable illustration cell totipotency. However, the precise molecular mechanisms regulating SE remain unclear. To characterize events this unique process, transcriptome analysis, in combination with biochemical histological approaches, were conducted cotton, typical plant species SE. Genome-wide profiling gene expression allowed identification novel...
The cambium and procambium generate the majority of biomass in vascular plants. These meristems constitute a bifacial stem cell population from which xylem phloem are specified on opposing sides by positional signals. PHLOEM INTERCALATED WITH XYLEM (PXY) receptor kinase promotes division organization. However, how these functions integrated is unknown. Here, we mapped putative PXY-mediated transcriptional regulatory network comprising 690 transcription factor-promoter interactions Arabidopsis (
Summary Fiber elongation is the key determinant of fiber quality and output in cotton ( G ossypium hirsutum ). Although expression profiling functional genomics provide some data, mechanism development still not well understood. Here, a gene encoding calcium sensor, h C M 7 , was isolated based on its high level relative to other s cells at fast stage. The wild‐type fuzzless/lintless mutant correspond presence absence, respectively, initials. Overexpressing promotes early elongation, whereas...
Summary As a product of asexual reproduction in plants, the somatic embryo ( SE ) differentiates into new plantlet via zygotic embryogenesis‐like process. Here, we present phenotypic and cellular differences between s embryos ZE s) revealed by histological section scanning using three parallel development stages two types cotton G ossypium hirsutum cv. YZ 1), including globular, torpedo cotyledonary‐stages. To identify molecular characteristics cotton, digital gene expression system was used...
Transcription factors operate as important switches of transcription networks, and NAC (NAM, ATAF, CUC) are a plant-specific family involved in multiple biological processes. However, this gene has not been systematically characterized cotton.Here we identify large number genes with conservative domains four cotton species, 147 found Gossypium arboreum, 149 G. raimondii, 267 barbadense 283 hirsutum. Predicted membrane-bound were also identified. Phylogenetic analysis showed that proteins...
Summary Drought resistance (DR) is a complex trait that regulated by variety of genes. Without comprehensive profiling DR‐related traits, the knowledge genetic architecture for DR in cotton remains limited. Thus, there need to bridge gap between genomics and phenomics. In this study, an automatic phenotyping platform (APP) was systematically applied examine 119 image‐based digital traits (i‐traits) during drought stress at seedling stage, across natural population 200 representative upland...
Abstract Background Somatic embryogenesis is a major process for plant regeneration. However, cell communication and the gene regulatory network responsible reprogramming during somatic are still largely unclear. Recent advances in single-cell technologies enable us to explore mechanism of regeneration at resolution. Results We generate high-resolution transcriptomic landscape hypocotyl tissue from highly regenerable cotton genotype Jin668 recalcitrant TM-1. identify nine putative clusters...
Summary N 6 ‐methyladenosine (m A) is the most prevalent internal modification present in mRNAs, and considered to participate a range of developmental biological processes. Drought response highly regulated at genomic, transcriptional post‐transcriptional levels. However, function regulatory mechanism m A drought stress still poorly understood. We generated transcriptome‐wide map using drought‐resistant drought‐sensitive varieties cotton under different water deficient conditions uncover...
JASMONATE ZIM-domain (JAZ) family proteins are involved in regulating diverse biological processes plants. However, their functions have not been well characterized cotton (Gossypium spp.). In the present study, 13, 15, 25 and 30 JAZ genes were identified Gossypium arboretum, raimondii, barbadense hirsutum, respectively, based on gene homology. Selection variation analyses showed that single nucleotide polymorphism (SNP) density of GhJAZ wild species was much higher than domesticated...
Somatic embryogenesis (S.E.) is a versatile model for understanding the mechanisms of plant and useful tool propagation. To decipher intricate molecular program potentially to control parameters affecting frequency S.E., proteomics approach based on two-dimensional gel electrophoresis (2-DE) combined with MALDI-TOF/TOF was used. A total 149 unique differentially expressed proteins (DEPs) were identified at different stages cotton S.E. compared initial (0 h explants). The expression profile...