A5082476019- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Tendon Structure and Treatment
- Angiogenesis and VEGF in Cancer
- Eicosanoids and Hypertension Pharmacology
- Sulfur Compounds in Biology
- Cancer Cells and Metastasis
- Advanced Materials and Mechanics
- Supramolecular Self-Assembly in Materials
- Micro and Nano Robotics
- Adhesion, Friction, and Surface Interactions
- Cerebrospinal fluid and hydrocephalus
- Electrohydrodynamics and Fluid Dynamics
- Wound Healing and Treatments
- Lipid Membrane Structure and Behavior
- Cardiovascular Disease and Adiposity
- Connective tissue disorders research
- RNA Research and Splicing
- Nuclear Structure and Function
- Cancer, Hypoxia, and Metabolism
- Renal and related cancers
- Polymer Surface Interaction Studies
- RNA Interference and Gene Delivery
University of Michigan
2020-2024
University of Washington
2018
Fibrous extracellular matrix (ECM) proteins provide mechanical structure and adhesive scaffolding to resident cells within stromal tissues. Aligned ECM fibers play an important role in directing morphogenetic processes, supporting loads, facilitating cell migration. Various methods have been developed align purified biopolymer hydrogels, such as type I collagen, including flow-induced alignment, uniaxial tensile deformation, magnetic particles. However, biopolymers limited orthogonal...
Synthetic hydrogels represent an exciting avenue in the field of regenerative biomaterials given their injectability, orthogonally tunable mechanical properties, and potential for modular inclusion cellular cues. Separately, recent advances soluble factor release technology have facilitated control over milieu cell microenvironments via microparticles. A composite hydrogel incorporating both these components can robustly mediate tendon healing following a single injection. Here, synthetic...
Abstract Capillary scale vascularization is critical to the survival of engineered 3D tissues and remains an outstanding challenge for field tissue engineering. Current methods generate micro‐scale vasculatures such as printing, two photon hydrogel ablation, angiogenesis, vasculogenic assembly face challenges in rapidly creating organized, highly vascularized at capillary length‐scales. Within metabolically demanding tissues, native beds are organized densely packed achieve adequate delivery...
We demonstrate the facile and robust generation of giant peptide vesicles by using an emulsion transfer method. These can sustain chemical physical stresses. The host cell-free expression reactions encapsulating essential ingredients. show incorporation another expressed elastin-like polypeptide into existing membrane vesicles.
Extracellular matrix (ECM) provides important biophysical and biochemical cues to maintain tissue homeostasis. Current synthetic hydrogels offer robust mechanical support for in vitro cell culture but lack the necessary protein ligand composition elicit physiological behavior from cells. This manuscript describes a fabrication method kidney cortex ECM-derived hydrogel with proper robustness supportive composition. The is fabricated by mechanically homogenizing solubilizing decellularized...
Collective cell migration is critical for proper embryonic development, wound healing, and cancer invasion. However, much of our knowledge has been performed using flat surfaces that lack topographical features do not recapitulate the complex fibrous architecture extracellular matrix (ECM). The recent availability synthetic networks designed to mimic in vivo ECM key identify topological dictate patterns as well determine underlying mechanisms regulate topography-sensing. Recent studies have...
Abstract Somatic cell fate is an outcome set by the activities of specific transcription factors and chromatin landscape maintained gene silencing alternate fates through physical interactions with nuclear scaffold. Here, we evaluate role scaffold as a guardian in human fibroblasts comparing effects transient loss (knockdown) mutation (progeria) functional Lamin A/C, core component We observed that A/C deficiency or disrupts morphology, heterochromatin levels, increases access to DNA...
ABSTRACT The peritumoral stroma is a complex 3D tissue that provides cells with myriad biophysical and biochemical cues. Histologic observations suggest during metastatic spread of carcinomas, these cues influence transformed epithelial cells, prompting diversity migration modes spanning single cell multicellular phenotypes. Purported consequences variations in tumor escape strategies include differential capability therapy resistance. Therefore, understanding how from the stromal...
Angiogenic programming in the vascular endothelium is a tightly regulated process to maintain tissue homeostasis and activated injury tumor microenvironment. The metabolic basis of how gas signaling molecules regulate angiogenesis elusive. Herein, we report that hypoxic upregulation NO synthesis endothelial cells reprograms transsulfuration pathway increases H
Vascularization of large, diffusion-hindered biomaterial implants requires an understanding how extracellular matrix (ECM) properties regulate angiogenesis. Sundry biomaterials assessed across many disparate angiogenesis assays have highlighted ECM determinants that influence this complex multicellular process. However, the abundance material platforms, each with unique parameters to model endothelial cell (EC) sprouting presents additional challenges interpretation and comparison between...
The peritumoral stroma is a complex 3D tissue that provides cells with myriad biophysical and biochemical cues. Histologic observations suggest during metastatic spread of carcinomas, these cues influence transformed epithelial cells, prompting diversity migration modes spanning single cell multicellular phenotypes. Purported consequences variations in tumor escape strategies include differential capability therapy resistance. Therefore, understanding how from the stromal microenvironment...