A5002486929- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- RNA Research and Splicing
- Extracellular vesicles in disease
- PI3K/AKT/mTOR signaling in cancer
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- 3D Printing in Biomedical Research
- Sirtuins and Resveratrol in Medicine
- Ubiquitin and proteasome pathways
- Nuclear Structure and Function
- Mitochondrial Function and Pathology
- Spaceflight effects on biology
- Single-cell and spatial transcriptomics
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Cancer-related molecular mechanisms research
University of Michigan
2020-2025
BioSurfaces (United States)
2020-2023
Cornell University
2019-2020
Volumetric muscle loss (VML) overwhelms the innate regenerative capacity of mammalian skeletal (SkM), leading to numerous disabilities and reduced quality life. Immune cells are critical responders injury guide tissue resident stem cell– progenitor-mediated myogenic repair. However, how immune cell infiltration intercellular communication networks with altered following VML drive pathological outcomes remains underexplored. Herein, we contrast cellular molecular mechanisms injuries that...
During aging, there is a progressive loss of volume and function in skeletal muscle that impacts mobility quality life. The repair regulated by tissue-resident stem cells called satellite (or [MuSCs]), but MuSCs decrease numbers regenerative capacity. transcriptional networks epigenetic changes confer diminished as result natural aging are only partially understood. Herein, we use an integrative genomics approach to profile from young aged animals before after injury. Integration these...
Adult stem cells decline in number and function old age identifying factors that can delay or revert age-associated adult cell dysfunction are vital for maintaining healthy lifespan. Here we show Vitamin A, a micronutrient is derived from diet metabolized into retinoic acid, acts as an antioxidant transcriptional regulator muscle cells. We first obstruction of dietary A young animals drives mitochondrial cycle mimics age. Next, pharmacologically targeted acid signaling myoblasts aged ex vivo...
Age-related skeletal muscle atrophy or sarcopenia is a significant societal problem that becoming amplified as the world's population continues to increase. The regeneration of damaged mediated by stem cells, but in old age cells become functionally attenuated. molecular mechanisms govern cell aging encompass changes across multiple regulatory layers and are integrated three-dimensional organization genome. To quantitatively understand how hierarchical chromatin architecture during aging, we...
During aging and neuromuscular diseases, there is a progressive loss of skeletal muscle volume function impacting mobility quality life. Muscle often associated with denervation resident stem cells (satellite or MuSCs); however, the relationship between MuSCs innervation has not been established. Herein, we administered severe trauma to transgenic murine model that permits MuSC lineage tracing. We show subset specifically engraft in position proximal junction (NMJ), synapse myofibers motor...
The health and homeostasis of skeletal muscle are preserved by a population tissue-resident stem cells (MuSCs) that maintain state mitotic metabolic quiescence in adult tissues. capacity MuSCs to preserve the quiescent declines with aging insults, promoting premature activation cell exhaustion. Sestrins class stress-inducible proteins act as antioxidants inhibit mammalian target rapamycin complex 1 (mTORC1) signaling complex. Despite these pivotal roles, role has not been explored cells. We...
The acute traumatic or surgical loss of skeletal muscle, known as volumetric muscle (VML), is a devastating type injury that results in exacerbated and persistent inflammation followed by fibrosis. mechanisms mediate the magnitude duration inflammatory response ensuing fibrosis after VML remain understudied, such, development regenerative therapies has been limited. To address this need, we profiled how lipid mediators, which are potent regulators immune injury, varied with injuries heal...
Abstract Muscle stem cells (MuSCs) are an essential adult cell population with the capacity to self-renew and regenerate muscle tissue. Functionally heterogeneous subpopulations of MuSCs have been identified based on their expression myogenic regulatory factors surface markers. However, a unified organization progenitor remains unresolved. Here, we performed temporal analysis skeletal regeneration using single-cell RNA-sequencing (scRNA-seq) myotoxin-injured mouse hindlimb muscles. We...
Abstract Somatic cell fate is an outcome set by the activities of specific transcription factors and chromatin landscape maintained gene silencing alternate fates through physical interactions with nuclear scaffold. Here, we evaluate role scaffold as a guardian in human fibroblasts comparing effects transient loss (knockdown) mutation (progeria) functional Lamin A/C, core component We observed that A/C deficiency or disrupts morphology, heterochromatin levels, increases access to DNA...
ABSTRACT Volumetric muscle loss (VML) overwhelms the innate regenerative capacity of mammalian skeletal (SkM), leading to numerous disabilities and reduced quality life. Immune cells are critical responders injury guide tissue resident stem cell progenitor mediated myogenic repair. However, how immune infiltration inter-cellular communication networks with altered following VML drive pathological outcomes remains underexplored. Herein, we contrast cellular molecular mechanisms injuries that...
Abstract Muscle stem cells (MuSCs) are an essential cell population for skeletal muscle homeostasis and regeneration throughout adulthood. MuSCs ideal candidate therapies chronic acute injuries diseases given their inherent ability to self-renew generate progenitor capable of myogenic commitment fusion. Given rarity propensity lose stem-cell potential in prolonged culture, methods ex vivo MuSC expansion that achieve clinical-scale yields represent a critical unmet need cell-therapeutic...
Abstract The health and homeostasis of skeletal muscle is preserved by a population tissue resident stem cells called satellite cells. Young healthy maintain state quiescence, but aging or metabolic insults results in reduced capacity to prevent premature activation cell exhaustion. As such, understanding genes pathways that protect maintenance quiescence are needed. Sestrins class stress-inducible proteins act as antioxidants inhibit the mammalian target rapamycin complex 1 (mTORC1)...
Abstract Age-related skeletal muscle atrophy or sarcopenia is a significant societal problem that becoming amplified as the world’s population continues to increase. A critical contributor loss in number and function of stem cells, which maintain tissue homeostasis regenerate damage. The molecular mechanisms govern cell aging encompass changes across multiple regulatory layers are integrated by three-dimensional organization genome. To quantitatively understand how hierarchical chromatin...