Abstract Nanofibrous materials are widely investigated as a replacement for the extracellular matrix, 3D foundation cells in all tissues. However, with every medical material, nanofibers too must pass safety evaluations like vitro cytotoxicity assays or vivo animal tests. Our literature research showed that differences results of used applied to evaluate nanofibrous poorly understood. To better explore this issue, we prepared three similar physical properties made poly-L-lactic acid,...

The growing pollution of the environment with slowly decomposing waste, as well increasing drug resistance pathogens, including antibiotic bacteria, has led to a search for new solutions based on biodegradable and natural materials, which are known their potential bacteriostatic properties. This study aimed produce nanofibers by blowing from polylactide (PLA) polymer solution containing compounds (e.g., beeswax, propolis). As result conducted research, were produced PLA various additives....

Abstract Comparative statistical analysis of the infiuence processing parameters, for electrospinning (ES) and solution blow spinning (SBS) processes, on nanofibrous poly(L-lactic acid) (PLLA) material morphology average fiber diameter was conducted in order to identify key parameter tailoring product properties. Further, a comparative preliminary biocompatibility evaluation performed. Based Design Experiment (DOE) principles, standard effects voltage, air pressure, feed rate concentration,...

Rapid endothelialization helps overcome the limitations of small-diameter vascular grafts. To develop biomimetic non-thrombogenic coatings supporting endothelialization, medical-grade polyurethane (PU) nanofibrous mats and tubular scaffolds with a diameter below 6 mm prepared by solution blow spinning were coated polydopamine (PDA), or PDA gelatin (PDA/Gel). The characterized scanning electron microscopy, porosity measurement, tensile testing, wettability, Fourier Transform Infrared...

The growing popularity of solution blow spinning as a method for the production fibrous tissue engineering scaffolds and vast range polymer–solvent systems available raises need to study effect processing conditions on fiber morphology develop its qualitative assessment. Rheological approaches determine polymer spinnability image analysis describe diameter alignment have been previously proposed, although in separate manner mostly widely known, well-researched electrospinning method. In this...

3D printing is a promising technique for producing bone implants, but there still need to adjust efficiency, facilitate production, and improve biocompatibility. Porous materials have proven positive effect on the regeneration of tissue, their production associated with numerous limitations. In this work, we described simple method polymer or polymer-ceramic filaments 3D-printing scaffolds by adding micrometer-scale porous structures scaffold surfaces. Scaffolds included polycaprolactone...

The results of synthesis the fluorescent carbon dots (CDots) from graphite target and reduced graphene oxide (rGO) nanoparticles performed by nanosecond laser ablation in polyethylene glycol 200 (PEG200) are shown. Two-step irradiation (first target, next achieved suspension) revealed a very effective production CDots. However, PEG appeared to be with 1064 nm pulse contrast 355 pulse. In case rGO similar procedure was less efficient. both cases, received exhibited strong, broadband...

Hydroxyapatite Ca10(PO4)6(OH)2 nanoparticles have been successfully synthesized by the wet chemical precipitation method at 60 °C in presence of biocompatible natural surfactant-lecithin. The composition and morphology hydroxyapatite with lecithin (nHAp-PC) was studied X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM). Size distribution for measured nanoparticle tracking analysis NanoSight system. We discuss details influence...

The use of nanofibrous materials in the field tissue engineering requires a fast, efficient, scalable production method and excellent wettability obtained materials, leading to enhanced cell adhesion. We proposed superhydrophilic two-step process. process is designed increase resulting scaffolds enhance rate fibroblast Polyurethane (PU) material was produced solution blow spinning Then PU fibers surface modified by dopamine polymerization water solution. Two variants modification were...

Chitosan (CS)/poly(ethylene oxide) (PEO)-based nanofiber mats have attracted particular attention as advanced materials for medical and pharmaceutical applications. In the scope of present studies, solution blow spinning was applied to produce nanofibers from PEO CS physicochemical biopharmaceutical studies were carried out investigate their potential wound nanomaterial skin healing regeneration. Additional coating with hydrophobic poly(dimethylsiloxane) favor removal surface. Unmodified...

In this study, fibrous polyurethane (PU) materials with average fiber diameter of 200, 500, and 1000 nm were produced using a solution blow spinning (SBS) process. The effects the rotation speed collector (in range 200-25 000 rpm) on alignment investigated. results showed that was influenced by collector, such possible when within specific range. Homogeneously oriented fibers obtained only for ≥500 nm. Moreover, changes in orientation (resulting from collector) more noticeable an comparison...

CP5 bovine chondrocytes were cultured on biodegradable electrospun fibrous polylactide (PLA) scaffolds placed a flexible interface formed between two immiscible liquid phases: (1) hydrophobic perfluorochemical (PFC) and (2) aqueous culture medium, as new way of cartilage implant development. Robust intensive growth cells was achieved in our hybrid liquid–solid–liquid system consisting the PLA contrast to limited traditional with scaffold solid surface. The multicellular aggregates covered...

The search for the perfect bone graft material is an important topic in science and medicine. Despite human being ideal material, due to its composition, morphology, familiarity with cells, autografts are widely considered demanding cause additional stress patient because of harvesting. However, from tissue banks can be used prepare materials eligible form transplantation. Without proteins fats, becomes a non-immunogenic matrix cells repopulate place implantation. To repair losses, granulate...

Currently, numerous studies are conducted using nanofibers as a scaffold for culture cardiac cells; however, there still needs to be more research evaluating the impact of physicochemical properties polymer on structure and function cells. We have studied how poly(

Heart diseases are caused mainly by chronic oxygen insufficiency (hypoxia), leading to damage and apoptosis of cardiomyocytes. Research into the regeneration a damaged human heart is limited due lack cellular models that mimic cardiac tissue. Based on literature, nanofibrous mats affect cardiomyocyte morphology stimulate growth differentiation cells cultured them; therefore, materials can support production in vitro faithfully 3D structure Nanofibrous were used as scaffolds for adult primary...

Regardless the low production rate, electrospinning remains attractive technique for nanofibers in various fields. Thus, development of a multi-jet technologies gives an opportunity to scale up and increase throughput fibers production. However, exhibit one major drawback– electrostatic mutual jet repulsion issue. In present research, we propose air blow-assisted system allowing with yield, at least, tenfold higher than single electrospinning. The produces two modes: case latter, application...

Abstract Control of the morphology and size particles in processes like crystallization precipitation remains challenging. We present simple process controlled hydroxyapatite nanoparticles remodeling into plate rod‐like morphologies. The precipitation/remodeling assumes that temperature pH change step affect properties resulting particles. were determined with scanning electron microscopy images. functional groups using Fourier Transform Infrared Spectroscopy, while phase composition...

Abstract Capillary scale vascularization is critical to the survival of engineered 3D tissues and remains an outstanding challenge for field tissue engineering. Current methods generate micro‐scale vasculatures such as printing, two photon hydrogel ablation, angiogenesis, vasculogenic assembly face challenges in rapidly creating organized, highly vascularized at capillary length‐scales. Within metabolically demanding tissues, native beds are organized densely packed achieve adequate delivery...

In this study, we have investigated how new composite material (magnetic polyurethane (PU) nanofibers) influences the culture and maturation of cardiomyocyte cells. Magnetic iron (II, III) oxide nanoparticles (Fe3O4, MNPs) were incorporated into nanofiber structure using solution blow spinning (SBS) method, resulting in magnetic mats. These mats characterized by physicochemical, optical (Young's modulus, wettability, surface zeta potential, autofluorescence), properties. It was found that...

Every bone implant to work correctly after implantation needs integrate with the surrounding bone.To enhance such a process, called osseointegration, various techniques of surface modification emerged.One approaches is based on deposition nano-and submicron materials surface.This paper presents solution blow spinning process for producing poly-L-lactic acid (PLLA)/ceramic fibrous composites designed be deposited directly onto orthopaedic implants prior increase osseointegration.We produced...