A5055777832- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Muscle metabolism and nutrition
- Pluripotent Stem Cells Research
- Cardiovascular Disease and Adiposity
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Telomeres, Telomerase, and Senescence
- Cancer-related gene regulation
- Endometrial and Cervical Cancer Treatments
- Mycobacterium research and diagnosis
- Animal Genetics and Reproduction
University of Georgia
2015-2025
Tsinghua University
2024
Chinese Research Academy of Environmental Sciences
2024
Capital Medical University
2022
Wuhan University
2022
Harbin Medical University
2020-2022
Third Affiliated Hospital of Harbin Medical University
2020-2022
Zhongnan Hospital of Wuhan University
2022
Beijing Chao-Yang Hospital
2022
Qingdao Municipal Hospital
2021
The interface between cellular systems involving small noncoding RNAs and epigenetic change remains largely unexplored in metazoans. RNA-induced silencing have the potential to target particular regions of genome for by locating specific sequences recruiting chromatin modifiers. Noting that several genes encoding RNA components been implicated regulation Drosophila , we sought a direct link system heterochromatin components. Here show PIWI, an ARGONAUTE/PIWI protein family member binds...
Abstract DNA N 6 -methyldeoxyadenosine (6mA) is a well-known prokaryotic modification that has been shown to exist and play epigenetic roles in eukaryotic DNA. Here we report 6mA accumulates up ∼0.1–0.2% of total deoxyadenosine during early embryogenesis vertebrates, but diminishes the background level with progression embryo development. During this process large fraction 6mAs locate repetitive regions genome.
The Argonaute/PIWI protein family consists of Argonaute and PIWI subfamilies. Argonautes function in RNA interference micro-RNA pathways; whereas PIWIs bind to PIWI-interacting RNAs regulate germ line development, stem cell maintenance, epigenetic regulation, transposition. However, the role mammalian cells has not been demonstrated, molecular mechanisms mediated by remain elusive. Here we show that MILI, a murine protein, is expressed cytoplasm testicular cells, spermatogonia, early...
Prdm16 determines the bidirectional fate switch of skeletal muscle/brown adipose tissue (BAT) and regulates thermogenic gene program subcutaneous white (SAT) in mice. Here we show that miR-133a, a microRNA is expressed both BAT SATs, directly targets 3′ UTR Prdm16. The expression miR-133a dramatically decreases along commitment differentiation brown preadipocytes, accompanied by upregulation Overexpression SAT cells significantly inhibits, conversely inhibition upregulates, adipogenesis....
Abstract Many inorganic nanoparticles are prepared and their behaviors in living systems investigated. Yet, common electrolytes such as NaCl left out of this campaign. The underlying assumption is that electrolyte will quickly dissolve water behave similarly constituent salts. Herein, preconception challenged. study shows (SCNPs) but not salts highly toxic to cancer cells. This because SCNPs enter cells through endocytosis, bypassing cell regulations on ion transport. When dissolved inside...
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials methods Data availability References Decision letter Author response author information Metrics During development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible are used extensively to identify genomic regulatory elements. Whilst landscapes pluripotent stem cells well characterised, accessibility the somatic lineages not defined. Here we...
The remarkable regeneration capability of skeletal muscle depends on the coordinated proliferation and differentiation satellite cells (SCs). self-renewal SCs is critical for long-term maintenance potential. Hypoxia profoundly affects proliferation, differentiation, cultured myoblasts. However, physiological relevance hypoxia signaling in vivo remains largely unknown. Here, we demonstrate that are an intrinsic hypoxic state express hypoxia-inducible factor 2A (HIF2A). HIF2A promotes stemness...
Abstract Human beige adipocytes (BAs) have potential utility for the development of therapeutics to treat diabetes and obesity-associated diseases. Although several reports described generation in vitro, their cell therapy drug discovery has not been reported. Here, we describe BAs from human adipose-derived stem/stromal cells (ADSCs) serum-free medium with efficiencies >90%. Molecular profiling shows them be similar primary isolated tissue. In exhibit uncoupled mitochondrial respiration...
Skeletal muscle differentiation is mediated by a complex gene expression program requiring both the muscle-specific transcription factor Myogenin (Myog) and p38α MAPK (p38α) signaling. However, relative contribution of Myog to formation mature myotubes remains unknown. Here, we have uncoupled activity from that gain insight into individual roles these proteins in myogenesis. Comparative profiling confirmed activates genes involved function. Furthermore, found absence signaling, leads...
Epigenetic research has been focused on cell-type-specific regulation; less is known about common features of epigenetic programming shared by diverse cell types within an organism. Here, we report a modified method for chromatin immunoprecipitation and deep sequencing (ChIP–Seq) its use to construct high-resolution map the Drosophila melanogaster key histone marks, heterochromatin protein 1a (HP1a) RNA polymerase II (polII). These factors are mapped at 50-bp resolution genome-wide 5-bp...
Pax7 is a nodal transcription factor that essential for regulating the maintenance, expansion, and myogenic identity of satellite cells during both neonatal adult myogenesis. Deletion results in loss impaired muscle regeneration. Here, we show ectopic expression constitutively active intracellular domain Notch1 (NICD1) rescues Pax7-deficient restores their proliferative potential. Strikingly NICD1-expressing do not undergo differentiation instead acquire brown adipogenic fate vivo vitro....