A5022580269- Muscle Physiology and Disorders
- RNA modifications and cancer
- Wheat and Barley Genetics and Pathology
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Plant Disease Resistance and Genetics
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Neurogenetic and Muscular Disorders Research
- Ubiquitin and proteasome pathways
- Genetics and Neurodevelopmental Disorders
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Galectins and Cancer Biology
- MicroRNA in disease regulation
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Plant Pathogens and Resistance
- Genetic Mapping and Diversity in Plants and Animals
- Cytokine Signaling Pathways and Interactions
- Advanced Proteomics Techniques and Applications
- Microtubule and mitosis dynamics
- Genetics and Plant Breeding
- Mitochondrial Function and Pathology
- Glioma Diagnosis and Treatment
University of Ottawa
2003-2025
Jewish General Hospital
2016-2025
McGill University
2018-2025
Centre For Human Genetics
2021-2022
Ottawa Hospital Research Institute
2011-2015
Ottawa Hospital
2011-2015
Health Canada
2010
Agriculture and Agri-Food Canada
2002-2006
Adult skeletal muscle regeneration is a highly orchestrated process involving the activation and proliferation of satellite cells, an adult stem cell. Activated cells generate transient amplifying progenitor pool myoblasts that commit to differentiation fuse into multinucleated myotubes. During regeneration, canonical Wnt signalling activated has been implicated in regulating myogenic lineage progression terminal differentiation.Here, we have undertaken gene expression analysis committed...
Glioblastoma contains a rare population of self-renewing brain tumor stem cells (BTSCs) which are endowed with properties to proliferate, spur the growth new tumors, and at same time, evade ionizing radiation (IR) chemotherapy. However, drivers BTSC resistance therapy remain unknown. The cytokine receptor for oncostatin M (OSMR) regulates proliferation glioblastoma tumorigenesis. Here, we report our discovery mitochondrial OSMR that confers IR via regulation oxidative phosphorylation,...
Abstract The function and maintenance of muscle stem cells (Mu SC s) are tightly regulated by signals originating from their niche environment. Skeletal myofibers a principle component the Mu in direct contact with cells. Here, we show that Myf6 establishes ligand/receptor interaction between associated fibers. Our data transcriptionally regulates broad spectrum myokines muscle‐secreted proteins skeletal myofibers, including EGF . EGFR signaling blocks p38 MAP kinase‐induced differentiation...
Abstract Adult stem cells are indispensable for tissue regeneration, but their function declines with age. The niche environment in which the reside plays a critical role function. However, quantification of effect on cell is lacking. Using muscle (MuSC) as model, we show that aging leads to significant transcriptomic shift subpopulations accompanied by locus-specific gain and loss chromatin accessibility DNA methylation. By combining vivo MuSC transplantation computational methods,...
Brain tumor stem cells (BTSCs) and intratumoral heterogeneity represent major challenges in glioblastoma therapy. Here, we report that the LGALS1 gene, encoding carbohydrate binding protein, galectin1, is a key regulator of BTSCs resistance to Genetic deletion alters BTSC gene expression profiles results downregulation sets associated with mesenchymal subtype glioblastoma. Using combination pharmacological genetic approaches, establish inhibition signaling impairs self-renewal, suppresses...
Myofibers are the main components of skeletal muscle, which is largest tissue in body. highly adaptive and can be altered under different biological disease conditions. Therefore, transcriptional epigenetic studies on myofibers crucial to discover how chromatin alterations occur muscle However, due heterogenous nature studying isolation proves a challenging task. Single-cell sequencing has permitted study epigenome isolated myonuclei. While this provides with high dimensionality, depth...
Skeletal muscle is a heterogeneous tissue. Individual myofibers that make up tissue exhibit variation in their metabolic and contractile properties. Although biochemical histological assays are available to study myofiber heterogeneity, efficient methods analyze the whole transcriptome of individual lacking. Here, we report on single-myofiber RNA-sequencing (smfRNA-Seq) approach by combining single-fiber isolation with Switching Mechanism at 5' end RNA Template (SMART) technology. Using...
Recent discoveries have shown that systemic manipulation, such as parabiosis, blood exchange, and young plasma transfer, can counteract many hallmarks of aging. This rejuvenation effect has been attributed to circulatory factors produced by cells from both hematopoietic non-hematopoietic lineages. However, the specific involvement bone marrow (BM) or in producing their effects on aging is still unclear. We developed a model aged mice with transplanted old BM assessed impact process,...
The cytokine receptor Oncostatin M (OSMR) plays diverse and important roles in several human malignancies, including brain, breast, pancreatic cancer. In glioblastoma (GB), OSMR orchestrates a feedforward loop with the oncogenic EGFRvIII STAT3, promoting tumor progression. Beyond EGFRvIII, promotes cancer stem cells via upregulation of mitochondrial oxidative phosphorylation (OXPHOS), contributes to therapy resistance5. However, molecular mechanisms governing multifaceted remain poorly...
<title>Abstract</title> Oncostatin M receptor (OSMR) plays diverse and important roles in several human malignancies, including brain, breast, pancreatic cancer<sup>1-4</sup>. Glioblastoma (GB) is the most malignant genetically brain tumour, with no cure. The common genetic mutation GB a truncated active mutant of epidermal growth factor (EGFR), EGFRvIII. OSMR orchestrates feedforward signaling mechanism EGFRvIII signal transducer activator transcription 3 (STAT3), to drive...
The plant homeodomain zinc-finger protein, PHF6, is a transcriptional regulator, and PHF6 germline mutations cause the X-linked intellectual disability (XLID) Börjeson-Forssman-Lehmann syndrome (BFLS). mechanisms by which regulates transcription how its BFLS remain poorly characterized. Here, we show genome-wide binding of in developing cortex vicinity genes involved central nervous system development neurogenesis. Characterization mice harbouring patient reveals an increase embryonic neural...
We used quantitative real-time PCR analysis to measure the copy number of BARE-1 retrotransposon in 5 cultivars barley (Hordeum vulgare), as well samples from its wild relative, Hordeum spontaneum. Two sets primers were amplify regions within long terminal repeat (LTR) and reverse transcriptase (RT) gene (GenBank accession Z17327). The LTR detected an average 2.148 × 10 ± 0.012 copies per haploid genome among samples, whereas RT 1.588 4 0.085 copies. ratio LTR:RT was estimated be 13.5:1....
Mice lacking p107 exhibit a white adipose deficiency yet do not manifest the metabolic changes typical for lipodystrophy, and instead low levels of serum triglycerides normal liver phenotype. When fed high fat diet, p107-null mice still did accumulate in liver, display markedly elevated energy expenditures together with an increased preference lipids. Skeletal muscle was therefore examined, as this is normally major tissue involved whole body lipid metabolism. Notably, p107-deficient express...