A5018185283- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Pesticide Exposure and Toxicity
- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Peroxisome Proliferator-Activated Receptors
- Nanoplatforms for cancer theranostics
- Enzyme Structure and Function
- Cancer-related gene regulation
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Nerve injury and regeneration
- Prostate Cancer Treatment and Research
- Nanoparticle-Based Drug Delivery
- Pesticide Residue Analysis and Safety
- Cholinesterase and Neurodegenerative Diseases
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Vascular Malformations and Hemangiomas
- HER2/EGFR in Cancer Research
- Lymphatic Disorders and Treatments
- Nicotinic Acetylcholine Receptors Study
- Trace Elements in Health
Fudan University
2011-2022
University of Georgia
2015-2021
Regeneron (United States)
2021
Georgia College & State University
2017-2020
Abstract Short-chain acylations of lysine residues in eukaryotic proteins are recognized as essential posttranslational chemical modifications (PTMs) that regulate cellular processes from transcription, cell cycle, metabolism, to signal transduction. Lysine butyrylation was initially discovered a normal straight chain (Knbu). Here we report its structural isomer, branched butyrylation, i.e. isobutyrylation (Kibu), existing new PTM on nuclear histones. Uniquely, isobutyryl-CoA is derived...
Androgen deprivation therapy has led to elevated cases of androgen receptor (AR) pathway-independent prostate cancer with dysregulated fatty acid metabolism. However, it is unclear how cells sustain metabolism drive AR-independent cancer. Long-chain acyl-CoA synthetases (ACSL) catalyze the conversion acids into acyl-CoAs that are required for In this study, we demonstrate expression levels ACSL3 and 4 were oppositely regulated by androgen-AR signaling in cells. AR served as a transcription...
Lymphatic metastasis can be greatly promoted by metastases growth and lymphangiogenesis in lymph nodes (LNs). LyP-1, a cyclic peptide, is able to specifically bind with tumor cells lymphatics metastatic LNs. This work aimed use LyP-1-conjugated liposomes (L-LS) loaded doxorubicin (DOX) (L-LS/DOX) suppress lymphatic inhibiting both L-LS were prepared exhibited sizes around 90 nm spherical morphology as characterized transmission electron microscopy. The vitro cellular studies showed that...
To establish a gene delivery system for brain targeting, low molecular weight polyethylenimine (PEI(10 K)) was modified with myristic acid (MC), and complexed DNA, yielding MC-PEI(10 K)/DNA nanoparticles successfully. The were observed to be successfully taken up by the brains of mice. transfection efficiency then investigated, both in vitro vivo expression is significantly higher than that unmodified PEI(10 nanoparticles. anti-glioblastoma effect K)/pORF-hTRAIL demonstrated survival time...
p300 and GCN5 are two representative lysine acetyltransferases (KATs) in mammalian cells. It was recently reported that they possess multiple acyltransferase activities including acetylation, propionylation, butyrylation of the ε-amino group residues histones non-histone protein substrates. Although thousands acetylated substrates acetylation sites have been identified by mass spectrometry-based proteomic screening, our knowledge about causative connections between individual KAT members...
Acyl-Coenzyme As (acyl-CoAs) are a group of activated fatty acid molecules participating in multiple cellular processes including lipid synthesis, oxidative metabolism acids to produce ATP, transcriptional regulation, and protein post-translational modification. Quantification acyl-CoAs is challenging due their instability aqueous solutions lack blank matrices. Here we demonstrate an LC-MS/MS analytical method which allows for absolute quantitation with broad coverage acyl-CoAs. This assay...
We encapsulated vincristine into folic acid-conjugated PEGylated liposomes to improve the anti-tumor efficacy on multidrug resistant cancers. It was observed that drug delivery system we constructed exhibited maximum cytotoxicity KBv200 cells (multidrug variant) compared with any other formulations. The semi-quantitative analysis of region interest revealed there a great increase in area under curve (AUC) near-infrared fluorescein solid tumors due acid-mediated accumulation. Folic showed...
Human neural progenitor cells are capable of independent, directed differentiation into astrocytes, oligodendrocytes and neurons thus offer a potential cell source for developmental neurotoxicity (DNT) systems. progenitor-derived astrocyte-neuron cocultured at defined ratios mimic cellular heterogeneity interaction in the central nervous system. Cytochrome P450 enzymes expressed relatively high level astrocytes may play critical role biotransformation endogenous or exogenous compounds,...
Abstract Numerous genetic alterations have been identified during prostate cancer progression. The influence of environmental factors, particularly the diet, on acceleration tumor progression is largely unknown. Expression levels and/or activity Src kinase are highly elevated in numerous cancers including advanced stages cancer. In this study, we demonstrate that high‐fat diets (HFDs) promoted pathological transformation mediated by synergy and androgen receptor vivo. Additionally, a diet...
Acetylation of α-tubulin at Lys-40 is a potential biomarker for cognitive deficits in various neurological disorders. However, this key post-translational modification (PTM) has not been previously studied with mass spectrometry, due to the inadequate distribution tryptic cleavage sites. Following peptic digestion, surrogate sequence containing PTM site was identified and found be stable quantitatively reproducible. A highly sensitive specific SISCAPA-LC-MS method quantitating rat brain...
Antibody–drug conjugates (ADCs) are biopharmaceuticals for the targeted delivery of antitumor agents. ADCs can be highly heterogeneous with various drug-to-antibody ratio (DAR) species, conjugation sites, and occupancy levels. The site modulate ADC stability efficacy therefore considered to a critical quality attribute (CQA) during development. Traditional mass spectrometry (MS)-based peptide mapping methods cannot accurately quantify site-specific conjugations due significant ionization...
Abstract Short-chain acylation of lysine residues in eukaryotic proteins are recognized as essential posttranslational chemical modifications (PTMs) that regulate cellular processes from transcription, cell cycle, metabolism, to signal transduction. Lysine butyrylation was initially discovered a normal straight chain (Knbu). Here we report its structural isomer, branched butyrylation, i.e. isobutyrylation (Kibu), existing new PTM on nuclear histones. Uniquely, isobutyryl-CoA is derived...