A5066523777- Renal cell carcinoma treatment
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cutaneous lymphoproliferative disorders research
- Immunotherapy and Immune Responses
- Thyroid Cancer Diagnosis and Treatment
- RNA modifications and cancer
- CAR-T cell therapy research
- Hematopoietic Stem Cell Transplantation
- Cancer-related molecular mechanisms research
- Lymphoma Diagnosis and Treatment
- Cancer Research and Treatments
- Cancer-related gene regulation
- Renal and Vascular Pathologies
- Pancreatic and Hepatic Oncology Research
- Nutrition, Genetics, and Disease
- Epigenetics and DNA Methylation
- Molecular Biology Techniques and Applications
- Cervical Cancer and HPV Research
- Radiomics and Machine Learning in Medical Imaging
- Circular RNAs in diseases
- Cancer Immunotherapy and Biomarkers
- Diabetes and associated disorders
- Dermatology and Skin Diseases
City of Hope
2012-2025
City Of Hope National Medical Center
2007-2025
Beckman Research Institute
2008-2023
Quantitative BioSciences
2023
Nanfang Hospital
2022
Diabetes Australia
2011
Chinese Academy of Medical Sciences & Peking Union Medical College
2006-2007
University of Colorado Health
2006
Starting at middle age, adults often suffer from visceral adiposity and associated adverse metabolic disorders. Lineage tracing in mice revealed that adipose progenitor cells (APCs) fat undergo extensive adipogenesis during age. Thus, despite the low turnover rate of adipocytes young adults, is unlocked Transplantations quantitatively showed APCs middle-aged exhibited high adipogenic capacity cell-autonomously. Single-cell RNA sequencing identified a distinct APC population, committed...
All-trans retinoic acid (ATRA), a potent derivative of vitamin A, can regulate immune responses. However, its role in inducing tolerance associated with the prevention islet inflammation and inhibition type 1 diabetes remains unclear.We investigated mechanisms underlying potential immunoregulatory effect ATRA on using an adoptive transfer animal model disease.Our data demonstrated that treatment inhibited NOD mice established insulitis. In addition, it suppressed interferon...
Diarrhea occurs in approximately half of patients with metastatic renal cell carcinoma (mRCC) receiving vascular endothelial growth factor-tyrosine kinase inhibitors (VEGF-TKI). We evaluated the relationship between VEGF-TKI-related diarrhea and stool microbiota.Stool samples were collected from 20 mRCC VEGF-TKIs. 16S rRNA sequencing was used to characterize bacteriomic profiling patients. Assay validation Salmonella typhimurium spike-in experiments suggested greatest speciation use V5...
Ten-eleven translocation (TET) family enzymes (TET1, TET2, and TET3) oxidize 5-methylcytosine (5mC) generate 5-hydroxymethylcytosine (5hmC) marks on the genome. Each TET protein also interacts with specific binding partners partly plays their role independent of catalytic activity. Although basic is well established now, molecular mechanism contribution noncatalytic domains remain elusive. Here, by combining in silico biochemical screening strategy, we have identified a small molecule...
Abstract Pevonedistat (TAK924) is a Nedd8-activating enzyme inhibitor with preclinical activity in non-Hodgkin lymphoma (NHL). This open-label, Phase I, multicenter, investigator-sponsored study enrolled patients relapsed/refractory (R/R) NHL and chronic lymphocytic leukemia (CLL). The primary objective was safety. given intravenously on days 1, 3, 5 of 21-day cycle for 8 cycles at five dose levels (15 to 50 mg/m 2 ); ibrutinib administered 420 or 560 mg orally daily continuously. Eighteen...
Thyroid cancer is frequently difficult to diagnose due an overlap of cytologic features between malignant and benign nodules. This leads unnecessary removal the thyroid in patients without cancer. While providing some improvement over cytopathologic diagnostics, molecular methods fail provide a correct diagnosis for These approaches are based on difference adjacent tissue assume that tissues same as However, contrast tissues, nodules can contain genetic alterations be found...
Cutaneous T cell lymphoma (CTCL) is a disfiguring and incurable disease characterized by skin-homing malignant cells surrounded immune that promote CTCL growth through an immunosuppressive tumor microenvironment (TME). Preliminary data from our phase I clinical trial of anti–programmed death ligand 1 (anti–PD-L1) combined with lenalidomide in patients relapsed/refractory demonstrated promising efficacy. In the current study, we analyzed TME, which revealed predominant PD-1+ M2-like...
CD4+ T cell interactions with B cells play a critical role in the pathogenesis of systemic autoimmune diseases such as lupus and chronic graft-versus-host disease (cGVHD). Extrafollicular CD44hiCD62LloPSGL1loCD4+ (PSGL1loCD4+ cells) are associated cGVHD, but their causal has not been established. With murine humanized MHC-/-HLA-A2+DR4+ models we showed that human PSGL1loCD4+ from GVHD target tissues have features helpers upregulated expression programmed death protein 1 (PD1) inducible...
Although the overall survival rate of B cell acute lymphoblastic leukemia (B-ALL) in childhood is more than 80%, it merely 30% refractory/relapsed and adult patients with B-ALL. This demonstrates a need for improved therapy targeting this subgroup Here, we show that ten-eleven translocation 1 (TET1) protein, dioxygenase involved DNA demethylation, overexpressed plays crucial oncogenic role independent its catalytic activity Consistent B-ALL, overexpression TET1 alone normal precursor cells...
Clonal hematopoiesis (CH), characterized by the accumulation of acquired somatic mutations in blood, is associated with an elevated risk aging-related diseases and premature mortality non-cancer populations. Patients who undergo autologous hematopoietic cell transplantation (HCT) are also at high onset conditions. Therefore, we examined association between pretreatment CH late-occurring (≥1 year) nonrelapse (NRM) after HCT. We evaluated pathogenic likely variants (PVs) 10 patients developed...
Abstract Efforts to improve the prognosis of steroid-resistant gut acute graft-versus-host-disease (SR-Gut-aGVHD) have suffered from poor understanding its pathogenesis. Here we show that pathogenesis SR-Gut-aGVHD is associated with reduction IFN-γ + Th/Tc1 cells and preferential expansion IL-17 − IL-22 Th/Tc22 cells. The causes dysbiosis in a Reg3γ-dependent manner. Transplantation IFN-γ-deficient donor CD8 T absence CD4 produces phenocopy SR-Gut-aGVHD. deficiency also leads PD-1-dependent...
Colorectal cancer (CRC) remains a significant cause of related mortality. Fat mass and obesity-associated protein (FTO) is m6A mRNA demethylase that plays an oncogenic role in various malignancies. In this study we evaluated the FTO CRC tumorigenesis.Cell proliferation assays were conducted 6 cell lines with inhibitor CS1 (50-3200 nM) (± 5-FU 5-80 mM) after lentivirus mediated knockdown. Cell cycle apoptosis HCT116 cells (24 h 48 h, 290 nM CS1). Western blot dot plot performed to assess...
STAT3 deficiency (STAT3-/-) in donor T cells prevents graft-versus-host disease (GVHD), but the impact on graft-versus-leukemia (GVL) activity and mechanisms of GVHD prevention remains unclear. Here, using murine models GVHD, we show that STAT3-/- induced only mild reversible acute while preserving GVL effects against nonsusceptible lymphoblastic leukemia (ALL) a cell dose-dependent manner. depended programmed death ligand 1/programmed protein 1 (PD-L1/PD-1) signaling. In target tissues,...
Both Foxp3 + regulatory T cells (Tregs) and antigen-expanded − Tregs play an important role in regulating immune responses as well preventing autoimmune diseases graft rejection. Molecular mechanisms modulating Treg function remain largely unclear, however. We report here on the expression of inhibitory killer cell Ig-like receptor, KIR3DL1, a nonobese diabetic (NOD) mouse-derived autoantigen-specific (2D2), which protects from type 1 diabetes (T1D) adoptive transfer experiments. This gene...
MicroRNAs (miRNAs) have recently demonstrated great potential and enormous promise in the diagnosis, prognosis therapy of various types cancer. In this study, we performed a comprehensive miRNA expression analysis omental metastasis serous ovarian carcinoma (SOC) using small RNA sequencing. Two hundred fifty-one aberrantly expressed miRNAs were identified, which clearly separated malignant omentum from normal omentum. Furthermore, profiles primary chemo-sensitive chemo-resistant/refractory...
Desmoplastic small round cell tumor (DSRCT) is a rare, highly aggressive, translocation-associated soft-tissue sarcoma that primarily affects children, adolescents, and young adults, with striking male predominance. It characterized by t(11;22) generating novel EWSR1-WT1 fusion gene. Secondary genomic alterations are rarely described.Tumor tissue from 83 DSRCT patients was assayed hybrid-capture based comprehensive profiling, FoundationOne® Heme next generation sequencing analysis of 406...
Autoreactive pathogenic T cells (Tpaths) and regulatory (Tregs) express a distinct gene profiles; however, the genes associated genetic/signaling pathways responsible for functional determination of Tpaths vs. Tregs remain unknown. Here we show that Skp2, an E3 ubiquitin ligase affects cell cycle control death, plays critical role in function diabetogenic Tregs. Down-regulation Skp2 converts them into Foxp3-expressing The suppressive Tpath-converted is dependent on increased production...
MicroRNAs (miRNAs) have been shown to hold prognostic value in acute myeloid leukemia (AML); however, the temporal dynamics of miRNA expression AML are poorly understood. Using serial samples from a mouse model generate time-series sequencing data, we first show that transcriptome undergoes state-transition during initiation and progression. We modeled as particle undergoing Brownian motion quasi-potential validated state-space accurately predict time an independent cohort mice. The critical...