A5100392945- Plant biochemistry and biosynthesis
- Natural Compounds in Disease Treatment
- Plant Gene Expression Analysis
- Phytochemical compounds biological activities
- Natural product bioactivities and synthesis
- Fungal Biology and Applications
- Traditional Chinese Medicine Analysis
- Photosynthetic Processes and Mechanisms
- Cell death mechanisms and regulation
- Biochemical and biochemical processes
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Ginseng Biological Effects and Applications
- Autophagy in Disease and Therapy
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Fungal and yeast genetics research
- Pharmacological Effects of Natural Compounds
- Bacterial Genetics and Biotechnology
- Bioactive natural compounds
- Salivary Gland Disorders and Functions
- Plant Reproductive Biology
- Connective Tissue Growth Factor Research
- Medicinal Plants and Neuroprotection
- Microbial metabolism and enzyme function
Beijing Academy of Agricultural and Forestry Sciences
2022-2024
Agricultural Genomics Institute at Shenzhen
2024
Chinese Academy of Agricultural Sciences
2024
Ministry of Agriculture and Rural Affairs
2022-2024
Northwest A&F University
2007-2023
Nantong University
2023
State Administration of Traditional Chinese Medicine of the People's Republic of China
2022
Ministry of Education of the People's Republic of China
2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2022
National Medical Products Administration
2022
Recently, traditional Chinese medicine and medicinal herbs have attracted more attentions worldwide for its anti-tumor efficacy. Celastrol Triptolide, two active components extracted from the herb Tripterygium wilfordii Hook F (known as Lei Gong Teng or Thunder of God Vine), shown effects. was identified a natural 26 s proteasome inhibitor which promotes cell apoptosis inhibits tumor growth. The effect mechanism Triptolide on prostate cancer (PCa) is not well studied. Here we demonstrated...
This study aimed to investigate the application potential of Portulaca oleracea extract (POE) in active packaging for preservation chilled meat. First, antioxidant capacity and ingredients POE were systematically studied. The results demonstrated that has excellent contains abundant compounds. Subsequently, antioxidant-active films based on chitosan starch containing different concentrations (CS/POE films) successfully developed. main physicochemical mechanical properties CS/POE...
Autophagy is a catabolic process widely conserved among eukaryotes that permits the rapid degradation of unwanted proteins and organelles through lysosomal pathway. The Serine/threonine protein kinase ULK2 (unc-51 like 2) plays an important regulatory role in autophagy thanks to its involvement mTOR-regulated-initiation downstream ATG protein-related progression this process. An increasing number miRNAs have been found modulate by targeting some genes. In study, we focus on mir-26b prostate...
Background People with diabetes are more likely to develop cardiovascular diseases. Lipotoxicity plays a key role in the development of diabetic cardiomyopathy. Panax notoginseng saponin (PNS) has been used treat and obesity. However, PNS cardiomyopathy remains unclear. Methods Results Diabetic db/db mice received high‐dose (200 mg/kg per day) or medium‐dose (100 by gavage for 12 weeks until week 36. Lipid accumulation cardiac function were detected possible mechanisms involved explored....
Tandem repeats (TRs) are genomic regions that tandemly change in repeat number, which often multiallelic. Their characteristics and contributions to gene expression quantitative traits rice largely unknown. Here, we survey TR variations based on 231 genome assemblies the pan-genome graph. We identify 227,391 multiallelic loci, including 54,416 absent from Nipponbare reference genome. Only 1/3 show strong linkage with nearby bi-allelic variants (SNPs, Indels PAVs). Using 193 panicle 202 leaf...
Phenolic acids are the main bioactive compounds in Salvia miltiorrhiza, which can be increased by salicylic acid (SA) elicitation. However, specific molecular mechanism remains unclear. The nonexpresser of PR genes 1 (NPR1) and its family members essential components SA signaling pathway. Here, we report an NPR protein, SmNPR4, that showed strong expression hairy root after treatment, acting as a negative moderator SA-induced phenolic biosynthesis S. miltiorrhiza (S. miltiorrhiza). Moreover,...
Phenolic acids are the main active ingredients in Salvia miltiorrhiza, which can be used for treatment of many diseases, particularly cardiovascular diseases. It is known that salicylic acid (SA) enhance phenolic content, but molecular mechanism its regulation still unclear. Nonexpresser PR genes 1 (NPR1) plays a positive role SA signaling pathway. In this study, we identified SmNPR1 gene responds to induction and systematically investigated function. We found positively affected...
Triptolide, an active compound extracted from Chinese herb Leigongteng (Tripterygium wilfordii Hook F.), shows a broad-spectrum of anticancer activity through its cytotoxicity. However, the efficacy triptolide on laryngocarcinoma rarely been evaluated, and mechanism by which triptolide-induced cellular apoptosis is still not well understood. In this study, we found that significantly inhibited HEp-2 cells proliferation, migration survivability. Triptolide induces cell cycle arrest at G1...
Abstract Background The contribution of mitogen-activated protein kinase (MAPK) cascades to plant growth and development has been widely studied, but this knowledge not yet extended the medicinal Salvia miltiorrhiza, which produces a number pharmacologically active secondary metabolites. Results In study, we performed genome-wide survey identified six MAPKKK kinases ( MAPKKKKs ), 83 MAPKK MAPKKKs nine MAPK MAPKKs ) 18 MAPKs in S. miltiorrhiza genome. Within each class genes, small...
Expression of the MAL genes required for maltose fermentation in Saccharomyces cerevisiae is induced by and repressed glucose. Maltose-inducible regulation requires permease MAL-activator protein, a DNA-binding transcription factor encoded MAL63 its homologues at other loci. Previously, we showed that Mig1 repressor mediates glucose repression gene expression. Glucose also blocks MAL-activator-mediated induction through Mig1p-independent mechanism refer to as inhibition. Here report...
Celastrol is an active triterpenoid compound derived from Tripterygium wilfordii which well-known as a traditional Chinese medicinal plant. Squalene synthase has vital role in condensing two molecules of farnesyl diphosphate to form squalene, key precursor biosynthesis. In the present study, T. squalene (TwSQS) was cloned followed by prokaryotic expression and functional verification. The open reading frame cDNA TwSQS 1242 bp encoding 413 amino acids. Bioinformatic phylogenetic analysis...
Triptolide, an important bioactive diterpenoid extracted from the plant Tripterygium wilfordii, exhibits many pharmacological activities. MYC2 transcription factor (TF) plays role in regulation of various secondary metabolites plants. However, whether TF could regulate biosynthesis triptolide T. wilfordii is still unknown. In this study, two homologous genes, TwMYC2a and TwMYC2b, were isolated hairy roots functionally characterized. The analyses phylogenetic tree subcellular localization...
Endive (Cichorium endivia L.) is a leafy vegetable in the Asteraceae family. Sesquiterpene lactones (STLs) endive leaves bring bitter taste that varies between varieties. Despite their importance breeding varieties with unique flavours, sesquiterpenoid biosynthesis pathways are poorly understood. We assembled chromosome-scale genome of 641 Mb contig N50 5.16 and annotated 46,711 protein-coding genes. Several gene families, especially terpene synthases (TPS) genes, expanded significantly C....
Tanshinones, the major bioactive components in Salvia miltiorrhiza Bunge (Danshen), are synthesized via mevalonic acid (MVA) pathway or 2-C-methyl-D-erythritol-4-phosphate (MEP) and downstream biosynthesis pathway. In this study, bacterial component lipopolysaccharide (LPS) was utilized as a novel elicitor to induce wild type hairy roots of S. miltiorrhiza. HPLC analysis revealed that LPS treatment resulted significant accumulation cryptotanshinone (CT) dihydrotanshinone I (DTI). qRT-PCR...
Miltiradiene synthase (MS) genes, TwTPS27a and TwTPS27b, are the key diterpene genes in biosynthesis of triptolide, which is an important medicinally active diterpenoid Tripterygium wilfordii. However, mechanism underlying regulation TwTPS27a/b triptolide remains unclear. In this study, promoters (1496 bp) TwTPS27b (1862 were isolated analyzed. Some hormone-/stress-responsive elements transcription factor (TF) binding sites predicted both promoters, might be responsible for TwTPS27a/b. The...
Aims Endoplasmic reticulum stress (ERS) has been proven to be associated with apoptosis and plays a critical role in the development of many diabetic complications. In pathogenesis cystopathy (DCP), ERS is still unclear. Our study aimed at investigation involvement ERS‐associated detrusor muscle streptozocin (STZ)‐induced rats. Methods At different timepoints (4, 8, 12, 16 weeks after induction type 1 rat models), hematoxylin & eosin (H&E) staining was performed assess histological...