A5100457306- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- DNA Repair Mechanisms
- RNA Research and Splicing
- Pharmacogenetics and Drug Metabolism
- Vibrio bacteria research studies
- Immune Response and Inflammation
- DNA and Nucleic Acid Chemistry
- Tuberculosis Research and Epidemiology
- Immune cells in cancer
- RNA regulation and disease
- Folate and B Vitamins Research
- RNA modifications and cancer
- Hormonal Regulation and Hypertension
- Dietary Effects on Health
- Dialysis and Renal Disease Management
- Aquaculture disease management and microbiota
- RNA and protein synthesis mechanisms
- Biosimilars and Bioanalytical Methods
- Transplantation: Methods and Outcomes
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Hematopoietic Stem Cell Transplantation
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2024
Capital Medical University
2013-2023
Beijing Chest Hospital
2023
Beijing Hospital
2013-2021
Shantou University
2015
Cancer Hospital of Shantou University Medical College
2015
Beijing Shijingshan Hospital
2013
Mount Sinai Hospital
1996
Lunenfeld-Tanenbaum Research Institute
1995-1996
University of Toronto
1996
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) infection, is currently the deadliest infectious disease in human that can evolve to severe forms. A comprehensive immune landscape for Mtb infection critical achieving TB cure, especially patients. We performed single-cell RNA transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing of 213,358 cells from 27 samples, including 6 healthy donors 21 active patients with varying severity (6 mild, moderate 9 cases). Two published...
AbstractThe proto-oncogene Fli-1 is a member of the ets family transcription factor genes. Its high expression in thymus and spleen presence DNA binding sites for number lymphoid cell-specific genes suggest that involved regulation lymphopoiesis. Activation gene by either chromosomal translocation or viral insertion leads to Ewing's sarcoma humans erythroleukemia mice, respectively. Thus, normally pathways cell growth differentiation. We have generated H-2Kk–Fli-1 transgenic mice overexpress...
Significance We had previously shown that 8-oxoG:A mismatch pairing can occur at both the DNA and RNA levels, thus causing base substitutions in translation errors, respectively. However, little attention has been paid to biological significance of oxidized mRNA or clearance mechanism for such lesions mammalian cells. Here, we developed a sensitive reporter system capture sequence-altered proteins are translated from 8-oxoG–containing mRNAs. Also, applied deep-sequencing method measure...
The proto-oncogene Fli-1 is a member of the ets family transcription factor genes. Its activation by either chromosomal translocation or proviral insertion leads to Ewing's sarcoma in humans erythroleukemia mice, respectively, preferentially expressed hematopoietic and endothelial cells. This expression pattern resembled that c-ets-1, another gene closely related physically linked Fli-1. We also generated germ line mutation homologous recombination embryonic stem Homozygous mutant mice...
Aim: Liquid biopsies analyzing cell-free DNA (cfDNA) methylation in plasma offer a noninvasive diagnostic for diseases, with the potential of aging biomarkers underexplored. Methods: Utilizing enzymatic methyl-seq (EM-seq), this study assessed cfDNA patterns blood from 35 healthy individuals. Results: It found signatures, including higher levels and variations fragment sizes, plus approximately 2000 age-related differentially methylated CpG sites. A biological age predictive model based on...
Nasopharyngeal carcinoma (NPC) is prevalent in Southern China and Southeast Asia, autoantibody signatures may improve early detection of NPC. In this study, serum levels autoantibodies against a panel six tumor-associated antigens (p53, NY-ESO-1, MMP-7, Hsp70, Prx VI, Bmi-1) Epstein-Barr virus capsid antigen-IgA (VCA-IgA) were tested by enzyme-linked immunosorbent assay training set (220 NPC patients 150 controls) validated validation (90 68 controls). We used receiver-operating...
Heart failure (HF) has become a global public health problem due to its unclear pathogenesis. Our previous studies have found that RNA oxidation is associated with the occurrence and development of variety chronic diseases in elderly, but whether related pathogenesis HF remains unclear. Male Dahl salt-sensitive rats (DSSR) were divided into 8% NaCl groups 0.3% groups. The blood pressure DSSR, HE staining cardiac tissue, function index colour Doppler echocardiography plasma N-terminal...
Nucleic acid oxidation plays an important role in the pathophysiology progress of a variety diseases. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), which originate from DNA RNA oxidation, were most widely used indicators for oxidative stress. The study investigated relation between 8-oxo-dGsn, 8-oxo-Gsn, CKD. 146 patients with CKD divided into five disease stages, their fasting blood morning urine collected. levels 8-oxo-dGsn 8-oxo-Gsn plasma...
Oxidatively generated damage to nucleic acids may play an important role in the pathophysiological processes of a variety diseases. 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) are oxidatively products DNA RNA, respectively. Our previous studies have suggested that amounts 8-oxo-dGsn 8-oxo-Gsn urine were considerably higher than other body fluid or tissue. The aim this study was investigate whether levels random samples consistent with those 24...
Abstract Cytochrome P450 2C19 (CYP2C19) allelic variants are thought to play an important part in inter‐individual variability drug metabolism. We evaluated the vitro hydroxylation of nebivolol by 31 CYP2C19 alleles identified a Chinese Han population recently. Wild‐type CYP2C19*1B and 30 isoforms were highly expressed insect cells, enzymatic activities towards characterized. Among alleles, most recombinant exhibited no or significantly low activity compared with CYP2C19*1B. Three variants,...
The molecule 8-oxo-7,8-dihydroguanine (8-oxoGua), an oxidized form of guanine, can pair with adenine or cytosine during nucleic acid synthesis. RNA sequences that contain 8-oxoGua cause translational errors lead to the synthesis abnormal proteins. Human Nudix type 5 (NUDT5), a MutT-related protein, catalyzes hydrolysis 8-oxoGDP 8-oxoGMP, thereby preventing misincorporation into RNA. To investigate biological roles NUDT5 in human fibroblast cells, we established cell lines decreased levels...
The new allele HLA‐B*55:83N differs from HLA‐B*55:02:01:01 by a single nucleotide.
HLA-DQB1*05:155 differs from HLA-DQB1*05:01:01 by one nucleotide substitution at position 474 (G>C).
HLA‐DRB1*07:01:22 differs from HLA‐DRB1*07:01:01:01 at position 244 (C>T) in exon 2.
HLA‐C*03:02:17 differs from HLA‐C*03:02:02:01 by one nucleotide substitution at position 393.
HLA-DQB1*06:02:29 differs from HLA-DQB1*06:02:01:01 by one nucleotide substitution at position 408 (C > T).
Abstract Background The major histocompatibility complex (MHC) in humans includes three classical class I loci (A, B, and C), which are important biomarkers for the transplantation of organs hematopoietic stem cells. In MHC, polymorphism is known to be extremely high while interlocus recombination rare. We report a rare between HLA-A HLA-H, was analyzed using next generation sequencing nanopore sequencing. Methods sample, genotypes HLA-A, C, DRB1, DQB1 were firstly determined methods...
Amino acid variants in protein may result deleterious effects on enzymatic activity. In this study we investigate the DNA activity of
Cytochrome P450 2D6 (CYP2D6) exhibits rich genetic polymorphism, and functional changes caused by variations are the key reasons for differences in substrate drug systemic exposure. Discovering novel variants defining their enzymatic kinetic characteristics can contribute to personalized application of drugs. In this study, a data chain variant-function-structure was established through population-based sequencing, baculovirus insect cell expression,
HLA-DRB1*13:241 differs from HLA-DRB1*13:02:01 by one nucleotide substitution at position 335.
HLA‐B*48:43 differs from HLA‐B*48:01:01:01 by two nucleotide substitutions at positions 981 and 986.
HLA‐B*40:01:51 differs from HLA‐B*40:01:01 by 2 nucleotide substitutions at position 72 and 126.
HLA‐A*02:07:10 differs from HLA‐A*02:07:01 by one nucleotide substitution at position 117.
HLA‐C*07:613 differs from HLA‐C*07:01:01:01 by one nucleotide substitution at position 454.