A5090826268- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Pancreatic function and diabetes
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Circular RNAs in diseases
- Circadian rhythm and melatonin
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Acute Ischemic Stroke Management
- Plant Physiology and Cultivation Studies
- Nanoplatforms for cancer theranostics
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Barrier Structure and Function Studies
- RNA Research and Splicing
- Atherosclerosis and Cardiovascular Diseases
- Natural product bioactivities and synthesis
- Neurological Disease Mechanisms and Treatments
- Melanoma and MAPK Pathways
Tianjin Huanhu Hospital
2015-2025
Nanjing University
2008-2025
Nanjing General Hospital of Nanjing Military Command
2024-2025
Nanjing Medical University
2025
Xuzhou Medical College
2025
Université Libre de Bruxelles
2024
Erasmus Hospital
2024
German Center for Diabetes Research
2024
Central South University
2014-2023
Xiangya Hospital Central South University
2014-2023
Most amino acids can be encoded by several synonymous codons, which are used at unequal frequencies. The significance of codon usage remains unclear. One hypothesis is that frequent codons translated relatively rapidly. However, there little direct, in vivo, evidence regarding codon-specific translation rates. In this study, we generate high-coverage data using ribosome profiling yeast, analyze a novel algorithm, and deduce events the A- P-sites ribosome. Different decoded different rates...
Autophagy of mitochondria, termed mitophagy, plays an important role in cerebral ischemia-reperfusion (IR) injury, but the mechanism is not yet clear. Tissue-type plasminogen activator (tPA) most thrombolytic drug clinical treatment ischemic stroke and has neuroprotective effects. Here, we explored effects tPA on neuronal apoptosis mitophagy following IR. We found that knocking out gene significantly aggravated brain injury increased mitochondrial damage. Exposure neurons to reduced severity...
The T-cell antigen CD28 provides a costimulatory signal that is required for proliferation. receptor zeta/CD3 engagement without ligation leads to state of nonresponsiveness/anergy, thereby implicating in the control peripheral tolerance foreign antigens or tumors. A key unresolved question has concerned mechanism by which generates intracellular signals. Phosphatidylinositol 3-kinase (PI 3-kinase) lipid kinase with Src-homology 2 (SH2) domain(s) binds platelet-derived growth factor...
It has been confirmed that mitochondrial impairment may underlie both sporadic and familial Parkinson’s disease (PD). Mitochondrial fission/fusion biogenesis are key processes in regulating homeostasis. Therefore, we explored whether the protective effect of resveratrol rotenone‐induced neurotoxicity was associated with biogenesis. The results showed could not only promote mass DNA copy number but also improve homeostasis neuron function rats PC12 cells damaged by rotenone. We observed...
T-cell activation requires cooperative signals generated by the antigen receptor zeta-chain complex (TCR zeta-CD3) and costimulatory CD28. CD28 interacts with three intracellular proteins-phosphatidylinositol 3-kinase (PI 3-kinase), T cell-specific protein-tyrosine kinase ITK (formerly TSK or EMT), between growth factor receptor-bound protein 2 son of sevenless guanine nucleotide exchange (GRB-2-SOS). PI GRB-2 bind to phosphotyrosine-based Tyr-Met-Asn-Met motif means intrinsic Src-homology...
In yeast, the G1 cyclin Cln3 promotes cell cycle entry by activating transcription factor SBF. mammals, there is a parallel system for in which dependent kinase (CDK) activates E2F/Dp. Here we show that regulates SBF at least two different pathways, one involving repressive protein Whi5, and second Stb1. The Rpd3 histone deacetylase complex also involved. binds to CLN2 promoter, removes previously bound Whi5 deacetylase. Adding extra copies of binding site delays Start, possibly titrating...
UHRF1, an epigenetic factor, is implicated in various cellular processes of tumorigenesis. However, the modulation UHRF1 expression human bladder cancer at post-transcriptional levels remains unclear. Here, we report that miR-124 suppresses to affect progression through competitive binding same region its 3'-UTR. We show compared with corresponding normal tissues, upregulated and downregulated demonstrating inverse correlation UHRF1. Quantitative PCR western blot assay demonstrated...
Low stringency hybridization screening of a rat genomic DNA library with previously described cDNA clone encoding voltage-gated potassium channel has resulted in the characterization member family, RGK5. An uninterrupted nucleotide sequence encodes protein 525 amino acids length, revealing that entire coding region resides on single exon. RGK5 transcripts are present both mouse thymus and brain, as determined by Northern blot analysis. RNA transcribed vitro from directs expression functional...
Identification of specific biomarkers for ischemic stroke is necessary due to their abilities improve treatment outcomes. Many studies have demonstrated the involvement microRNAs (miRNAs) in pathogenesis and complications patient We found that expression miR-874-3p was downregulated clinical samples stroke. Thus present study explored potential role related mechanisms. A mouse model constructed by middle cerebral artery occlusion. The relationship among miR-874-3p, C-X-C motif chemokine...
Abstract Background It has been documented that aerobic exercise (AE) a positive effect on improving cognitive function in type 2 diabetes (T2DM) patients. Here, we tried to explore how AE regulates the expression of long non-coding RNA serum-exosomes (Exos), thereby affecting impairment T2DM mice as well its potential molecular mechanism. Methods mouse models were constructed, and serum-Exos isolated for whole transcriptome sequencing screen differentially expressed lncRNA mRNA, followed by...
Introduction: Ischemic stroke (IS) significantly alters brain cellular composition and gene expression, particularly in glial cells like astrocytes (ASTs), oligodendrocytes (OLs), oligodendrocyte precursor (OPCs). While these cells' roles neural repair are recognized, the precise molecular mechanisms remain unclear. This study aims to elucidate IS-induced transcriptomic changes ASTs, OLs, OPCs using single-cell RNA sequencing (scRNA-seq) of human tissues. We hypothesize that IS triggers...
Objective: Sialic acid-binding immunoglobulin-like lectin (siglec)-9 is a type Ⅰ transmembrane protein that plays an important role in intrinsic immunity. In this study, we examined the siglec-9 expression levels mononuclear macrophages, neutrophils and plasma from peripheral blood of acute respiratory distress syndrome (ARDS) patients investigated its clinical value. Methods: Peripheral was obtained ARDS (n=54) admitted to Department Respiratory Critical Care Medicine, Nanjing Jinling...
p73 is structurally and functionally related to p53 possibly a tumor suppressor gene. Using 15 surgically resected frozen esophageal specimens containing both squamous cell carcinomas (ESCC) neighboring normal epithelia, we studied gene alterations mRNA expression. Loss of heterozygosity the loci was found in nine 14 informative cases (64%). A polymorphism at codon 173 (Thr) identified (eight samples had ACC seven ACT), but mutation not detected samples. Nine ESCC (60%) displayed...
Whi3 is an RNA binding protein known to bind the mRNA of yeast G1 cyclin gene CLN3. It inhibits CLN3 function, but mechanism this inhibition unclear; in previous studies, made no observable difference levels, translation, or abundance. Here, we re-approach issue using microarrays, RNA-Seq, ribosome profiling, and other methods. By multiple methods, find that whi3 mutation causes a small consistent increase abundance hundreds mRNAs, including mRNA. The effect on various mRNAs roughly...
Pancreas injury by partial duct ligation (PDL) activates a healing response, encompassing β-cell neogenesis and proliferation. Macrophages (MΦs) were recently shown to promote proliferation after PDL, but they remain poorly characterized. We assessed myeloid cell diversity the factors driving dynamics following acute pancreas PDL. In naive sham-operated pancreas, compartment consisted mainly of two distinct tissue-resident MΦ types, designated MHC-II(lo) MHC-II(hi) MΦs, latter being...
Maf1 is a conserved effector of the mechanistic target rapamycin (mTOR), an aging promoting kinase. However, whether required for lifespan extension caused by mTOR inhibition, such as dietary restriction (DR) or calorie (CR) remains elusive. Here we show that deletion maf1 in budding yeast S. cerevisiae but not mafr-1 C. elegans prevents DR CR to extend lifespan. Interestingly, increases stress tolerance and extends MAFR-1 phosphorylated mTOR-dependent manner alleviates inhibition tRNA...
Abstract Primary defects in folding of mutant proinsulin can cause dominant‐negative accumulation the endoplasmic reticulum (ER), impaired anterograde trafficking, perturbed ER homeostasis, diminished insulin production, and β‐cell dysfunction. Conversely, if primary impairment ER‐to‐Golgi trafficking (which also perturbs homeostasis) drives misfolding nonmutant proinsulin—this might suggest bi‐directional entry into a common pathological phenotype (proinsulin misfolding, deficient export...
The development of atherosclerosis (AS) is a multifactorial process, in which elevated plasma resistin (a key factor leading to insulin resistance) levels play an important role. Emerging evidence indicate that microRNAs (miRNAs) are involved AS; However, the regulation and function miRNAs response AS remain poorly understood. Our study analyzed effects miR-492 on resistance, endothelial activation, expression apoE knock-out mice human umbilical vein cells after high-glucose treatment mimics...
Astragaloside IV (AS-IV), the major active component extracted from Astragalus membranaceus, has been demonstrated to exhibit protective effects on cardiovascular, immune, digestive and nervous systems; thus, widely used in traditional Chinese medicine. Abnormal proliferation migration of vascular smooth muscle cells (VSMCs) is closely associated with initiation progression cardiovascular diseases, including atherosclerosis restenosis. However, AS-IV VSMCs remain unknown. For first time,...
<b><i>Purpose:</i></b> Lactate dehydrogenase A (LDHA), which functions as a crucial enzyme in transforming from pyruvate into lactate, has been reported to be overexpressed various advanced cancer and its silencing turned out tumor suppressive. Previous studies have showed that the expression of LDHA was higher renal cell carcinoma (RCC) than corresponding normal tissue. However, function RCC, possible mechanism progression, not investigated....