A5022278574- Pesticide Exposure and Toxicity
- Heme Oxygenase-1 and Carbon Monoxide
- Autophagy in Disease and Therapy
- Carcinogens and Genotoxicity Assessment
- Coenzyme Q10 studies and effects
- Sirtuins and Resveratrol in Medicine
- Lipid metabolism and biosynthesis
- Parkinson's Disease Mechanisms and Treatments
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Vitamin C and Antioxidants Research
- Neuroscience and Neuropharmacology Research
- Trace Elements in Health
- DNA Repair Mechanisms
- Retinoids in leukemia and cellular processes
- Science Education and Pedagogy
- Immune cells in cancer
- Peroxisome Proliferator-Activated Receptors
- Liver Disease Diagnosis and Treatment
- Mosquito-borne diseases and control
- Cholinesterase and Neurodegenerative Diseases
- Inflammasome and immune disorders
- Neurological Disease Mechanisms and Treatments
- Toxic Organic Pollutants Impact
- Macrophage Migration Inhibitory Factor
Army Medical University
2015-2025
State Council of the People's Republic of China
2019
It has been confirmed that mitochondrial impairment may underlie both sporadic and familial Parkinson’s disease (PD). Mitochondrial fission/fusion biogenesis are key processes in regulating homeostasis. Therefore, we explored whether the protective effect of resveratrol rotenone‐induced neurotoxicity was associated with biogenesis. The results showed could not only promote mass DNA copy number but also improve homeostasis neuron function rats PC12 cells damaged by rotenone. We observed...
Abstract Nitrogen mustard (NM) causes severe skin injury with an obvious inflammatory response, which is lack of effective and targeted therapies. Vitamin D3 (VD3) has excellent anti‐inflammatory properties considered as a potential candidate for the treatment NM‐induced dermal toxicity; however, underlying mechanisms are currently unclear. Cyclooxygenase‐2 (COX2; widely used marker inflammation) plays key role in cutaneous inflammation. Herein, we initially confirmed that NM markedly...
Abstract Nitrogen mustard (NM) causes severe vesicating skin injury, which lacks effective targeted therapies. The major limitation is that the specific mechanism of NM-induced injury not well understood. Recently, autophagy has been found to play important roles in physical and chemical exposure-caused cutaneous injuries. However, whether contributes dermal toxicity unclear. Herein, we initially confirmed NM dose-dependently caused cell death induced keratinocytes. Suppression by...
Abstract The term “geographical synthetic thinking” is taken from the latest Chinese version of “Geography Curriculum Standards” (General High School 2017 edition, Compulsory Education 2022 edition) and one four core competencies prioritized in teaching geography at secondary-school level. This study proposes that using geographic subject mind maps improves students’ geographical thinking develops a set map (GSMM) drawing standards for teachers matching suggestions applications. Seven their...
Nitrogen mustard (NM) causes severe skin injury that is lack of effective and targeted therapies. Vitamin D3 (VD3) emerges as a promising treatment option for NM-caused dermal toxicity; however, the underlying mechanisms are currently unclear. Herein, we identified NM markedly promoted ferroptosis by measurement decreased cell viability, glutathione, glutathione peroxidase 4 solute carrier family 7 member 11 levels, increased ROS, lipid iron/Fe2+ malondialdehyde contents in vitro vivo....
Rotenone is a natural compound from plants. It widely used in pesticides because of highly toxic to insects and fish. However, lots research has reported that rotenone neurotoxic effects humans. confirmed there correlation between exposure Parkinson's disease (PD). Therefore, the role gut microbiota related metabolic pathways was investigated rotenone-induced neurotoxicity. The results showed abundance changed significantly. differential metabolites were enriched nicotinate nicotinamide...
Rotenone is an inhibitor of mitochondrial complex I that produces a model Parkinson's disease (PD), in which neurons undergo dopamine release dysfunction and other features. In neurons, exocytosis one the processes associated with dependent on Ca(2+) dynamic changes cell. present study, we have investigated involvement PC12 cells administrated rotenone. Results demonstrated rotenone led to elevation intracellular through influx by opening voltage-gated channel influenced soluble...
Nitrogen mustard (NM) is a chemotherapeutic agent capable of alkylating nucleophilic proteins and DNA, causing severe cell damage. However, no reports have been on the dynamic changes in proteomics induced by NM. In this study, we established model acute exposure to NM for 1 h continuous cultured 24 after removal (repair stage) using 16HBE cells. The nuclear protein spectrum crosslinked with DNA were analyzed, function p97 during damage was examined. An hour resulted into nucleus, which...
Abstract Nitrogen mustard (NM) belongs to vesicant agents. Blisters are one of the important characteristics NM skin damage. It is urgent further elucidate mechanism and develop effective countermeasures for damage induced by NM. The endoplasmic reticulum (ER) an intracellular organelle, playing role in maintaining cellular homeostasis. In this study, we explored stress (ERS) protective effect asiatic acid (AA) HaCaT cells was found that key regulatory proteins ERS, such as glucose regulated...
Abstract Sulfur mustard (a type of vesicant) can directly damage lung bronchial epithelium via aerosol inhalation, and prevalent cell death is an early event that obstructs the respiratory tract. JNK/c-Jun a stress response pathway, but its role in injured cells not clear. Here, we report was activated immortalized human epithelial (HBE) exposed to lethal dose (20 μM) nitrogen (NM, sulfur analog). c-Jun silencing using small-interfering RNA (siRNA) rendered resistant NM-mediated by blocking...
Using sulfur mustard analog 2-chloroethyl ethyl sulfide (CEES), we established an in vitro model by poisoning cultured immortalized human bronchial epithelial cells. Nile Red staining revealed lipids accumulated 24 h after a toxic dose of CEES (0.9 mM). Lipidomics analysis showed most the increased were triglycerides (TGs), and increase TGs was further confirmed using Triglyceride-Glo™ Assay kit. Protein mRNA levels DGAT1, important TG biogenesis enzyme, following 0.4 mM exposure. Under...
SM (Sulfur mustard) is an oily, hydrophobic, and lipophilic chemical agent that damages cells with intricate patterns. CEES (2-chloroethyl ethyl sulfide soluble) a standard analog commonly employed in the toxicity mechanism study. To obtain ideal results vitro, researchers should disperse well medium, avoiding presence of undissolved droplets. However, such purpose not easy to reach under conventional solution preparation, information about droplet formation function little available. Here,...
Abstract Nitrogen mustard (NM) can alkylate nucleophilic proteins and DNA, causing severe cell damage. However, there are no reports on NM-induced proteomics dynamic changes. In this study, nuclear cytoplasmic of 16HBE were separated the components amounts detected analyzed. The amount DNA protein cross-linking (DPC) function p97 also explored. One-hour-NM-exposure caused a tremendous number entered into nucleus DPC formation. As repair progressed, exited. Although influx at 1 h was delayed...
There is currently no satisfactory therapy for nitrogen mustard (NM)-caused dermal toxicity, poor understanding of the exact mechanisms. Autophagy has been found to play important roles in physical or chemical exposure-caused cutaneous injuries. Herein, we initially confirmed that NM dose-dependently caused cell death keratinocytes. The LC3B2 (light chain 3 beta 2) formation and SQSTM1/p62 (sequestosome 1) degradation autophagosomes were also upregulated by NM. And chloroquine treatment...
Background: Medical defense against chemical, biological, radiological, and nuclear (CBRN) threats should be established through systematic education in college career development training to counter the response successfully. Methods: Taking course of Countermeasures CBRN Weapons is compulsorily for all military medical students Chinese People’s Liberation Army (PLA). In present study, a modularized field Chemical Defense Medicine provided, which an important part Integrated Military...