A5028641640- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Molecular Biology Techniques and Applications
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Sexual Differentiation and Disorders
- Renal and related cancers
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- ATP Synthase and ATPases Research
- CAR-T cell therapy research
University of California, San Diego
2015-2024
La Jolla Alcohol Research
2012-2019
National Heart Lung and Blood Institute
2019
National Institutes of Health
2019
University of California San Diego Medical Center
2017
The University of Texas MD Anderson Cancer Center
2005-2014
University of Puerto Rico, Medical Sciences Campus
2013
Indiana University School of Medicine
2012
University of Virginia
2007
University of California, Los Angeles
2006
Highlights•Neonatal and adult human testicular cell subsets are defined by scRNA-seq analysis•The neonate contains both primordial germ cell- SSC-like cells•Numerous spermatogonial states with distinct markers defined•Neonatal "niche" cells factors definedSummarySpermatogenesis has been intensely studied in rodents but remains poorly understood humans. Here, we used single-cell RNA sequencing to analyze testes. Clustering analysis of neonatal testes reveals several subsets, including...
Gene rearrangement during the ontogeny of T- and B-cells generates an enormous repertoire T-cell receptor (TCR) immunoglobulin (Ig) genes. Because error-prone nature this process, two-thirds rearranged TCR Ig genes are expected to be out-of-frame thus contain premature terminations codons (ptcs). We performed sequence analysis reverse transcriptase-polymerase chain reaction products from fetal adult thymus found that newly transcribed TCR-β pre-mRNAs (intron-bearing) frequently derived...
Expression of most RNA polymerase II transcripts requires the coordinated execution transcription, splicing, and 3′ processing. We have previously shown that upon transcriptional activation a gene in vivo, pre-mRNA splicing factors are recruited from nuclear speckles, which they concentrated, to sites transcription (Misteli, T., J.F. Cáceres, D.L. Spector. 1997. Nature. 387:523–527). This recruitment process appears spatially coordinate within cell nucleus. Here we investigated molecular...
The mechanisms dictating whether a cell proliferates or differentiates have undergone intense scrutiny, but they remain poorly understood. Here, we report that UPF1, central component in the nonsense-mediated RNA decay (NMD) pathway, plays key role this decision by promoting proliferative, undifferentiated state. UPF1 acts, part, destabilizing NMD substrate encoding TGF-β inhibitor SMAD7 and stimulating signaling. also promotes of mRNAs many other proteins oppose Neural differentiation is...
Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions can augment or diversify existing functions. Here, we report gene event that yielded another outcome--the generation antagonistic One product this event--UPF3B--is critical nonsense-mediated RNA decay (NMD) pathway, while its autosomal counterpart--UPF3A--encodes an enigmatic protein previously shown to have trace NMD activity. Using loss-of-function approaches...