A5042657495- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Gut microbiota and health
- Cancer-related molecular mechanisms research
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Immune Response and Inflammation
- Cystic Fibrosis Research Advances
- Influenza Virus Research Studies
- Genetics and Neurodevelopmental Disorders
- Cancer Cells and Metastasis
- DNA and Nucleic Acid Chemistry
- Digestive system and related health
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Photosynthetic Processes and Mechanisms
- Neurogenetic and Muscular Disorders Research
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Hepatitis Viruses Studies and Epidemiology
New York Blood Center
2024
Jiangxi Agricultural University
2023
China University of Mining and Technology
2023
Ionis Pharmaceuticals (United States)
2013-2022
Nanjing Agricultural University
2015-2021
University of Chinese Academy of Sciences
2021
Ningbo University
2018
Ningbo No. 2 Hospital
2018
University of California, San Diego
2011-2017
Nanjing University of Posts and Telecommunications
2014
Abstract Background The p53 transcription factor is located at the core of a complex wiring signaling pathways that are critical for preservation cellular homeostasis. Only recently it has become clear regulates expression several long intergenic noncoding RNAs (lincRNAs). However, relatively little known about role lincRNAs play in this pathway. Results Here we characterize lincRNA named Pint (p53 induced transcript). We show ubiquitously expressed finely regulated by p53. In mouse cells,...
The mechanisms dictating whether a cell proliferates or differentiates have undergone intense scrutiny, but they remain poorly understood. Here, we report that UPF1, central component in the nonsense-mediated RNA decay (NMD) pathway, plays key role this decision by promoting proliferative, undifferentiated state. UPF1 acts, part, destabilizing NMD substrate encoding TGF-β inhibitor SMAD7 and stimulating signaling. also promotes of mRNAs many other proteins oppose Neural differentiation is...
Gene duplication is a major evolutionary force driving adaptation and speciation, as it allows for the acquisition of new functions can augment or diversify existing functions. Here, we report gene event that yielded another outcome--the generation antagonistic One product this event--UPF3B--is critical nonsense-mediated RNA decay (NMD) pathway, while its autosomal counterpart--UPF3A--encodes an enigmatic protein previously shown to have trace NMD activity. Using loss-of-function approaches...
Porcine epidemic diarrhea virus (PEDV) has catastrophic impacts on the global pig industry. Although fecal-oral route is generally accepted, an increased number of reports indicate that airborne transmission may contribute to PEDV outbreak. Here, we show could cause typical in piglets through a nasal spray. Firstly, can develop transient epithelium infection. Subsequently, PEDV-carrying dendritic cells (DCs) allow be transferred CD3+ T via virological synapse. Finally, virus-loaded reach...
The recessive genetic disease cystic fibrosis (CF) is caused by loss-of-function mutations in the CFTR (CF transmembrane conductance regulator) gene. Approximately 10% of patients with CF have at least one allele a nonsense mutation CFTR. Nonsense generate premature termination codons that can subject mRNA transcripts to rapid degradation through nonsense-mediated decay (NMD) pathway. Currently, there are no approved therapies specifically target Here, we identified antisense...
The loss of postharvest storability pummelo fruit reduces its commodity value for long run. To maintain storability, the effects dipping treatment by salicylic acid (SA) with different concentrations (0, 0.1, 0.2, or 0.3%) were investigated on (Citrus maxima Merr. cv. Jinshayou) during room temperature storage at 20 ± 2°C 90 d.Among all treatments, pre-storage SA 0.3% demonstrated most significant ability to reduce decay incidence, decrease weight loss, delay peel color-turned process, and...
Food allergy has posed a major global health burden due to the rising prevalence and lack of effective prophylactic strategies. During past decades, allergen-specific immunotherapy (AIT) been used as disease-modifying therapy for food allergic conditions, but long-term treatment duration, poor patient compliance unexpected adverse reactions, only minority patients benefit from AIT therapy. In this proof-of-concept study, using well-established mRNA platform, we developed vaccine candidates...
About 11% of all human genetic diseases are caused by nonsense mutations that generate premature translation termination codons (PTCs) in messenger RNAs (mRNA). PTCs not only lead to the production truncated proteins, but also often result decreased mRNA abundance due nonsense-mediated decay (NMD). Although pharmacological inhibition NMD could be an attractive therapeutic approach for treatment mutations, regulates expression 10–20% normal transcriptome. Here, we investigate whether can...
Abstract Approximately 10% of cystic fibrosis patients harbor nonsense mutations in the transmembrane conductance regulator ( CFTR ) gene which can generate codons mRNA and subsequently activate nonsense-mediated decay (NMD) pathway resulting rapid degradation. However, it is not known NMD branches govern mRNAs containing codons. Here we utilize antisense oligonucleotides targeting factors to evaluate regulation codon-containing by pathway. We observe that with G542X, R1162X, W1282X, but...
ABSTRACT Influenza whole inactivated virus (WIV) is more immunogenic and induces protective antibody responses compared with other formulations, like split or subunit vaccines, after intranasal mucosal delivery. Polyethyleneimine (PEI), an organic polycation, widely used as a reagent for gene transfection DNA vaccine Although PEI recently has demonstrated potent adjuvant activity viral glycoprotein antigens, its immune H9N2 WIV not well demonstrated. Here, mice were immunized intranasally...
Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets and causes substantial economic losses the world. PED virus (PEDV) spreads by fecal-oral contact can be prevented oral immunization. Therefore, it necessary to develop an effective vaccine against PEDV infection. Currently, Bacillus subtilis as recombinant carrier has been used for antigen delivery proved well immune effect safety. The present study evaluated immunogenicity of (B. subtilis-RC) via...
Liver regeneration after partial hepatectomy (PHx) is a complex and well-orchestrated biological process in which synchronized cell proliferation induced response to the loss of liver mass. To define long noncoding RNAs (lncRNAs) that participate regulation regeneration, we performed microarray analysis identified more than 400 lncRNAs exhibiting significantly altered expression. Of these, one lncRNA, LncPHx2 (Long RNA by PHx 2), was highly upregulated during regeneration. Depletion using...
ABSTRACT The spread of the low-pathogenicity avian H9N2 influenza virus has seriously increased risk a new pandemic. Although whole inactivated (WIV) vaccine via intranasal pathway is effective method blocking transmission, mucosal barrier seems to be major factor hampering its development. CpG oligodeoxynucleotides, known adjuvant, can target downstream dendritic cells (DCs) and effectively enhance systemic immune responses. However, ability CpGs assist WIV in transepithelial transport...