A5086744916- Plant Stress Responses and Tolerance
- Cystic Fibrosis Research Advances
- Subcritical and Supercritical Water Processes
- Free Radicals and Antioxidants
- Advanced biosensing and bioanalysis techniques
- Cellular transport and secretion
- Chromium effects and bioremediation
- Proteins in Food Systems
- RNA modifications and cancer
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Biochemical and biochemical processes
- Metalloenzymes and iron-sulfur proteins
- Fungal and yeast genetics research
- Plant Reproductive Biology
- Respiratory viral infections research
- Aluminum toxicity and tolerance in plants and animals
- Neurogenetic and Muscular Disorders Research
- Analytical chemistry methods development
- Gas Sensing Nanomaterials and Sensors
- Environmental Toxicology and Ecotoxicology
- Bee Products Chemical Analysis
- bioluminescence and chemiluminescence research
- Mercury impact and mitigation studies
- AI in cancer detection
Beijing Technology and Business University
2024-2025
Minzu University of China
2020-2025
Wenzhou Medical University
2016-2024
First Affiliated Hospital of Wenzhou Medical University
2023-2024
Cystic Fibrosis Foundation
2016-2023
Dalian Institute of Chemical Physics
2022
Chinese Academy of Sciences
2022
State Key Laboratory of Food Science and Technology
2022
Jiangnan University
2022
Lishui City People's Hospital
2016
Abstract Premature termination codons (PTCs) prevent translation of a full-length protein and trigger nonsense-mediated mRNA decay (NMD). Nonsense suppression (also termed readthrough) therapy restores function by selectively suppressing at PTCs. Poor efficacy current readthrough agents prompted us to search for better compounds. An NMD-sensitive NanoLuc reporter was used screen 771,345 Among the 180 compounds identified with activity, SRI-37240 its more potent derivative SRI-41315, induce...
Abstract Plants can grow in soils containing highly variable amounts of mineral nutrients, like Ca2+ and Mn2+, though the mechanisms adaptation are poorly understood. Here, we report first genetic study to determine vivo functions a pump plants. Homozygous mutants Arabidopsis harboring T-DNA disruption ECA1 showed 4-fold reduction endoplasmic reticulum-type calcium activity. Surprisingly, phenotype mutant plants was indistinguishable from wild type when grown on standard nutrient medium 1.5...
To understand the structure, role, and regulation of individual Ca 2+ pumps in plants, we have used yeast as a heterologous expression system to test function gene from Arabidopsis thaliana (ECA1). ECA1 encoded 116-kDa polypeptide that has all conserved domains common P-type (EC 3.6.1.38 ). The amino acid sequence shared more identity with sarcoplasmic/endoplasmic reticulum (53%) than plasma membrane (32%) pumps. Yeast mutants defective Golgi pump ( pmr1 ) or both vacuolar pmc1 cnb1 were...
Rationale: Premature termination codons (PTCs) in the cystic fibrosis transmembrane conductance regulator (CFTR) gene cause (CF). Several agents are known to suppress PTCs but poorly efficacious or toxic.Objectives: To determine whether there clinically available that elicit translational readthrough and improve CFTR function sufficient confer therapeutic benefit patients with CF PTCs.Methods: Two independent screens, firefly luciferase CFTR-mediated transepithelial chloride assay, were...
The recessive genetic disease cystic fibrosis (CF) is caused by loss-of-function mutations in the CFTR (CF transmembrane conductance regulator) gene. Approximately 10% of patients with CF have at least one allele a nonsense mutation CFTR. Nonsense generate premature termination codons that can subject mRNA transcripts to rapid degradation through nonsense-mediated decay (NMD) pathway. Currently, there are no approved therapies specifically target Here, we identified antisense...
The impact of pH and temperature on the volatile organic compounds (VOCs) produced from Maillard reaction between l-ascorbic acid (ASA) glycine (Gly) was investigated in hot compressed water (HCW) study. results showed that VOCs generated were mainly derivatives pyrazines, pyrroles, pyridines. effects generation pyridines different. higher would promote pyrrole pyridine derivatives, while effect not obvious to pyrazine derivatives. For some they only detected under 210 °C. solution influence...
To investigate how calmodulin regulates a unique subfamily of Ca(2+) pumps found in plants, we examined the kinetic properties isoform ACA2 identified Arabidopsis. A recombinant was expressed yeast K616 mutant deficient two endogenous pumps. Orthovanadate-sensitive (45)Ca(2+) transport into vesicles isolated from transformants demonstrated that is pump. pumping by full-length protein (ACA2-1) 4- to 10-fold lower than N-terminal truncated ACA2-2 (Delta2-80), indicating domain normally acts...
To identify and characterize individual Ca2+ pumps, we have expressed an Arabidopsis ECA1 gene encoding endoplasmic reticulum-type Ca2+-ATPase homolog in the yeast (Saccharomyces cerevisiae) mutant K616. The (pmc1pmr1cnb1) lacks a Golgi vacuolar membrane pump grows very poorly on Ca2+-depleted medium. Membranes isolated from showed high H+/Ca2+-antiport but no Ca2+-pump activity. Expression of endomembranes increased growth by 10- to 20-fold 45Ca2+ pumping into vesicles transformants was...
Cystic fibrosis (CF) is a hereditary disease caused by mutations in the gene coding for cystic transmembrane conductance regulator (CFTR). A large number of nearly 2000 reported mutations, including premature termination codon (PTC) urgently require new and personalized medicines. We have developed cell-based assays readthrough modulators CFTR PTC (or nonsense mutation suppressors), based on trafficking surface expression CFTR. Approximately 85,000 compounds been screened two (Y122X W1282X)....
Hot compressed water (HCW), also known as subcritical (SCW), refers to high-temperature in a special physical and chemical state. It is an emerging technology for natural product extraction. The volatile organic compounds (VOCs) generated from the Maillard reaction between l-ascorbic acid (ASA) l-cysteine (Cys) have attracted significant interest flavor fragrance industry. This study aimed explore formation mechanism of VOCs ASA Cys examine effects parameters such temperature, time, pH HCW.
The whole-cell bioreporters based on the cop-operon sensing elements have been proven specifically useful in assessment of bioavailable copper ions water environments. In this study, a series experiments was conducted to further improve sensitivity and robustness bioreporters. First, an Escherichia coli △copA△cueO△cusA mutant with three transport genes knocked out constructed. Then, copAp::gfpmut2 element inserted into chromosome E. by gene knock-in method obtain bioreporter strain WMC-007....
Abstract The non-enzymatic browning (NEB) reaction between l -ascorbic acid (ASA) and glycine (Gly), including the effects of temperature (110–150 °C), time (10–150 min) pH (4.5, 6.8, 8.0 9.5) on formation un-colored intermediate products (UIPs), (BPs) volatile (VPs), were investigated. results showed that had a remarkable effect reaction. characteristics zero-order kinetics for UIPs BPs discussed, corresponding activation energy ( E ) was also calculated. When 4.5, approximate 53.76 kJ/mol...
This study investigates the functional and conformational changes in highland barley proteins (HBPs)-salidroside complexes formed by non-covalent bond interactions. The free sulfhydryl contents of HBPs are decreased from 37.65 ± 1.90 μmol/g protein to 18.86 0.14 after binding with salidroside. fluorescence spectroscopy show that two peaks observed salidroside enhance intensity at high addition level (0.5–1 mmol/g protein). Secondary structure, surface hydrophobicity, Zeta potential also...
The impacts of interaction between proanthocyanidin (PC) and decolorized highland barley protein (DHBP) at pH 7 9 on the functional conformational changes in DHBP were investigated. It was shown that PC strongly quenched intrinsic fluorescence primarily through static quenching. mainly bound by hydrophobic interactions. Additionally, free sulfhydryl groups surface hydrophobicity obviously decreased after combining with PC. zeta potential DHBP–PC complexes increased significantly. A change...
Hepatocellular carcinoma (HCC) is one of the most prevalent and common malignant tumors worldwide, accounting for 85-90 % primary liver cancer cases. Accumulating evidence shows that long non-coding RNAs (LncRNAs) play regulatory roles in HCC occurrence progression. However, little known about biological role LncRNA "E2F1-regulated inhibitor cell death" (ERICD) HCC. Our study revealed ERICD highly expressed correlates with TNM staging; high levels were associated poor patient prognoses. We...
Escherichia coli [2Fe-2S]-ferredoxin and other ISC proteins encoded by the iscRSUA-hscBA-fdx-iscX (isc) operon are responsible for assembly of iron-sulfur clusters. It is proposed that ferredoxin (Fdx) donates electrons from its reduced [2Fe-2S] center to cluster biogenesis reactions. However, underlying mechanisms in Fdx remain elusive. Here, we report preferentially binds iron, but not cluster, under cold stress conditions (≤16°C). The iron binding characterized a unique absorption peak at...
The effect of reaction conditions, which comprised the temperature (150–190°C), processing time (0.50, 0.75, 1.00, 1.25, 1.50, 2.00, and 2.50 h), pH (5.0, 7.0, 9.5), concentration (0.03–0.07 mol/L) L -ascorbic acid (ASA), on degradation ASA was investigated in hot-compressed water (HCW). kinetics generation browning products (BPs) were studied. results showed that conformed to pseudo-first-order kinetics, formation BPs closely related H 3 O + HCW. acidic condition (pH = 5.0) lower (0.03 more...