A5078330016- Ferroptosis and cancer prognosis
- Metalloenzymes and iron-sulfur proteins
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Graphene and Nanomaterials Applications
- Folate and B Vitamins Research
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Mycobacterium research and diagnosis
- Biochemical and Molecular Research
- Epigenetics and DNA Methylation
- Electrochemical Analysis and Applications
- Cancer, Lipids, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- Cardiac Ischemia and Reperfusion
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Cancer-related gene regulation
- Metabolomics and Mass Spectrometry Studies
- Trace Elements in Health
- Nanoparticle-Based Drug Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Immune cells in cancer
- Nitric Oxide and Endothelin Effects
Huazhong Agricultural University
2025
Wenzhou Medical University
2014-2024
Ministry of Education of the People's Republic of China
2024
Hunan Agricultural Products (China)
2023
Peking University
2006-2023
Beijing Chest Hospital
2019-2023
Hunan Agricultural University
2018-2022
Academy of Military Medical Sciences
2018
South China Normal University
2015-2017
University of Oxford
2017
Increasing evidence suggests that the gut microbiota plays vital roles in metabolic diseases. Polygonatum odoratum extract alleviates hyperglycemia and hyperlipidemia, but underlying mechanism remains unclear. This study investigated effects of P. polysaccharides (POPs) on high-fat diet (HFD)-induced obesity rats whether these were related to modulation microbiota. POP treatment attenuated weight gain, fat accumulation, epididymal adipocyte size, liver triglycerides, total cholesterol...
Obesity leads to colonic inflammation and may increase the risk of colorectal cancer. Xylooligosaccharide (XOS) exhibits strong antioxidant excellent antibacterial properties, can be utilized by gut microbes maintain ecological balance intestinal tract. In this study, we explored how XOS modulates microbiota regulates high fat diet (HFD) induced inflammation. We measured changes in body weight visceral coefficients rats fed a high-fat diet. also expression levels inflammatory factors plasma...
Summary Among the iron‐sulphur cluster assembly proteins encoded by gene iscSUA ‐ hscBA fdx in E scherichia coli , IscA has a unique and strong iron binding activity can provide for vitro . Deletion of its paralogue SufA results an mutant that fails to assemble [4 F e‐4 S ] clusters under aerobic conditions, suggesting crucial role biogenesis. Here we report among proteins, also specific Cu ( I ) vivo The centre is stable resistant oxidation conditions. Mutation conserved cysteine residues...
Throughout a long evolutionary history, prokaryotes have co-evolved with bacteriophages. To resist phage invasion, developed diverse range of prokaryotic immunity. The immune system primarily operates through four mechanisms: inhibiting adsorption, blocking nucleic acid injection, degrading acids, and mediating abortive infection phages. Among the numerous mechanisms anti-phage defense systems, some systems based on mechanism, such as certain subtypes CRISPR-Cas system, Argonaute (pAgo),...
Background Hepatocellular carcinoma (HCC) is the most prevalent liver cancer, with a 5-year survival rate below 20% and an average time of 3-6 months. Identifying new biomarkers crucial for early diagnosis prognosis. The function PDZ domain protein 11 (PDZD11) in HCC remains unclear. Methods In this study, PDZD11 was investigated as potential biomarker using bioinformatic analysis TCGA ICGC datasets. Furthermore, we assessed serum clinical diagnostic marker by enrolling cohort comprising 78...
Graphene oxide (GO) has great potential in biomedical applications due to its excellent photothermal effect and drug loading.
Rosiglitazone (RG) is a well-known activator of peroxisome proliferator-activated receptor-gamma (PPAR γ ) and used to treat hyperglycemia type 2 diabetes; however, its clinical application has been confounded by adverse side effects. Here, we assessed the roles chlorogenic acid (CGA), phenolic secondary metabolite found in many fruits vegetables, on differentiation lipolysis mouse 3T3-L1 preadipocytes. The results showed that CGA promoted vitro according oil red O staining quantitative...
Significance Ras activation has been detected in many types of human cancers, and its activity can be regulated by association with GTPase-activating proteins (GAPs). However, the underlying mechanism regulation GAP is incompletely understood. Here we show that FAM129B phosphorylation EGFR reduced p120-RasGAP H-Ras, thereby enhancing H-Ras activation, Warburg effect, tumorigenesis. Given overexpressed cancer approaches to directly target have not successful, disruption interfering function...
In this study, a physiologically stable dual-polymer-functionalized reduced nanographene oxide (nrGO) conjugate (PEG-nrGO-PEI, RGPP) with high efficiency of gene delivery is successfully synthesized through mixing PEGylated (PEG-nGO, GP) and polyethylenimine (PEI, 25 kDa) solution under 80 °C for 2 h. This hydrothermal reduction GP during PEIylation promotes the nucleophilic reaction between amino moieties PEI epoxy groups (or carboxylic groups) in then forms C-NH- NH-CO to covalently...
// Yilin Pang 1 , Zihao Mai Bin Wang Lu Liping Wu Xiaoping 2 and Tongsheng Chen MOE Key Laboratory of Laser Life Science & College Biophotonics, South China Normal University, Guangzhou 510631, PR Department Pain Management, The First Affiliated Hospital Jinan 510630, Correspondence to: Chen, email: chentsh@scnu.edu.cn chentsh126@126.com Wang, txp2938@jnu.edu.cn Keywords: artesunate, nano-graphene oxide, chemo-photothermal synergistic therapy, peroxynitrite, near-infrared irradiation...
Abstract Background LYRM4 is necessary to maintain the stability and activity of human cysteine desulfurase complex NFS1-LYRM4-ACP. The existing experimental results indicate that cancer cells rely on high expression NFS1. However, role in liver hepatocellular carcinoma (LIHC) remains unclear. Methods In this study, we combined bioinformatics analysis clinical specimens evaluate mRNA, protein expression, gene regulatory network LIHC. Furthermore, detected several classical iron-sulphur...
Background: Peptidyl-prolyl isomerase H (PPIH) is a member of the cyclophilin protein family, which functions as molecular chaperone and involved in splicing pre-mRNA. According to reports, malignant progression HCC related hepatitis B virus (HBV) tightly associated with RNA-binding proteins. Nevertheless, there no research on PPIH expression or its function occurrence HCC. Results: We are first reveal that mRNA levels Ppih substantially overexpressed HCC, outcomes show. A significant...
Abstract Background The significance of tumor-secreted cytokines in tumor development has gained substantial attention. Nevertheless, the precise role tumor-related inflammatory prostate cancer (PCa) remains ambiguous. Objectives To gain deeper insights into response process PCa. Methods A total 233 cases were collected, including 80 hyperplasia as disease control, 65 postoperative and 36 PCa group. Additionally, 52 patients undergoing physical examinations during same period collected...
Mesenchymal stem cells (MSCs) have been used in hematopoietic cell transplantation for years. However, the safety of MSCs applied various types hematologic malignancy has not comprehensively explored. In present study, effects human umbilical cord-derived mesenchymal (hUC-MSCs) on six representative lines were explored, including leukemia, multiple myeloma and lymphoma cells. Direct indirect co-culture models established, proliferation was assessed by carboxyfluorescein diacetate...
The whole-cell bioreporters based on the cop-operon sensing elements have been proven specifically useful in assessment of bioavailable copper ions water environments. In this study, a series experiments was conducted to further improve sensitivity and robustness bioreporters. First, an Escherichia coli △copA△cueO△cusA mutant with three transport genes knocked out constructed. Then, copAp::gfpmut2 element inserted into chromosome E. by gene knock-in method obtain bioreporter strain WMC-007....
At present, approximately 30% of eukaryotic proteins can be expressed in a soluble form Escherichia coli. In this study, pCold-SUMOa plasmid was constructed order to express heterologous fused with SUMO by cold-shock expression vector. The human cysteine desulfurase NFS1 and chimeric namely, EH-IscS were successfully E. particularly difficult produced functionally, due their readily sequestered nature. recombinant desulfurases that generated exhibited higher activity, solubility stability...
Abstract The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional approaches, including affinity purification, gel filtration, and ion-exchange chromatography, require complex manipulation steps are costly. Here, we describe simple rapid strategy which SUMO tag Ulp1 protease surface-displayed separately on Escherichia coli cells. After induction, cells harvested, resuspended cleavage buffer, incubated...
Dihydroartemisinin (DHA) and artesunate (ARS), two artemisinin derivatives, have efficacious anticancer activities against human hepatocarcinoma (HCC) cells. This study aims to the action of combination treatment DHA/ARS farnesylthiosalicylic acid (FTS), a Ras inhibitor, in HCC cells (Huh-7 HepG2 cell lines). FTS pretreatment significantly enhanced DHA/ARS-induced phosphatidylserine (PS) externalization, Bak/Bax activation, mitochondrial membrane depolarization, cytochrome c release,...