A5023356478- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Graphene and Nanomaterials Applications
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Autophagy in Disease and Therapy
- Superconducting and THz Device Technology
- Carbon Dioxide Capture Technologies
- Catalysts for Methane Reforming
- Membrane-based Ion Separation Techniques
- Photoreceptor and optogenetics research
- Catalysis and Oxidation Reactions
- Lipid Membrane Structure and Behavior
- Physics of Superconductivity and Magnetism
- Inflammatory mediators and NSAID effects
- Cancer-related Molecular Pathways
- Parkinson's Disease Mechanisms and Treatments
- Electrochemical sensors and biosensors
- Drug Transport and Resistance Mechanisms
- Animal Genetics and Reproduction
- Ferroptosis and cancer prognosis
- Phagocytosis and Immune Regulation
- Coal Combustion and Slurry Processing
Southwest Jiaotong University
2021-2025
GeneCopoeia (United States)
2024
South China Normal University
2017-2022
Key Laboratory of Guangdong Province
2018
First Affiliated Hospital of Guangzhou Medical University
2018
Guangzhou Medical University
2018
Guangzhou Institute of Dermatology
2018
Guangzhou University
2017
South China University of Technology
2013-2016
We study the multi-photon energy resolution and demonstrate photon counting up to about 30 photons at near-infrared wavelengths in a kinetic inductance detector made from aluminum (Al) film. The has lumped-element design comprising large interdigitated capacitor parallel with narrow inductive strip. A fiber-coupled lens is used focus light onto absorber minimize scattering. Detectors different designs film thicknesses are studied. From histogram of optimally filtered response pulse height,...
// Yilin Pang 1 , Zihao Mai Bin Wang Lu Liping Wu Xiaoping 2 and Tongsheng Chen MOE Key Laboratory of Laser Life Science & College Biophotonics, South China Normal University, Guangzhou 510631, PR Department Pain Management, The First Affiliated Hospital Jinan 510630, Correspondence to: Chen, email: chentsh@scnu.edu.cn chentsh126@126.com Wang, txp2938@jnu.edu.cn Keywords: artesunate, nano-graphene oxide, chemo-photothermal synergistic therapy, peroxynitrite, near-infrared irradiation...
We recently developed a polyethylenimine (PEI) and polyethylene glycol (PEG) dual-functionalized reduced graphene oxide (GO) (PEG−nrGO−PEI, RGPP) for high-efficient gene delivery in HepG2 Hela cell lines. To evaluate the feasibility applicability of RGPP as carrier, we here assessed transfection efficiency on plasmids siRNA 11 different Commercial polyalkyleneimine cation reagent (TR) was used comparison. In cells, exhibited much stronger ability large size than TR. For green fluorescent...
We here introduce a fluorescence resonance energy transfer (FRET) two-hybrid assay method to measure the maximal donor(D)- and acceptor(A)-centric FRET efficiency (ED,max EA,max) of D-A complex its stoichiometry by linearly fitting donor-centric (ED) acceptor-to-donor concentration ratio (RC) acceptor-centric (EA) 1/RC, respectively. performed this on wide-field microscope for living HepG2 cells co-expressing tandem constructs free donor/acceptor obtained correct ED, EA, values those...
Abstract Metformin (Met) exhibits anticancer ability in various cancer cell lines. This report aims to explore the exact molecular mechanism of Met-induced apoptosis HCT116 cells, a human colorectal line. reactive oxygen species (ROS) increase and ROS-dependent death accompanied by plasma membrane blistering, mitochondrial swelling, loss potential, release cytochrome c. Western blotting analysis showed that Met upregulated Bak expression but downregulated Bax expression. Most importantly,...
Acceptor-sensitized quantitative Förster resonance energy transfer (FRET) measurement (E-FRET) is mainly impeded by donor emission crosstalk and acceptor direct excitation crosstalk. In this paper, we develop a novel E-FRET approach (Lux-E-FRET) based on linear unmixing (Lux) of the fluorescence intensity ratio between two detection channels with each different wavelengths. The need not to selectively collect or acceptor, wavelengths excite acceptor. For tandem FRET sensor, Lux-E-FRET only...
Bcl-2 family proteins, as central players of the apoptotic program, participate in regulation mitochondrial network. Here, a quantitative live-cell fluorescence resonance energy transfer (FRET) two-hybrid assay was used to confirm homo-/hetero-oligomerization mitofusins 2 and 1 (MFN2 MFN1), also demonstrate binding MFN2 MFN1 with 1:1 stoichiometry. A FRET for living cells co-expressing CFP-labeled Bcl-XL (an anti-apoptotic protein encoded by BCL2L1) YFP-labeled or demonstrated Neither nor...
Summary We recently developed two quantitative fluorescence resonance energy transfer (FRET) measurement methods based on spectral unmixing of emission spectra (IIem‐spFRET) and excitation–emission (ExEm‐spFRET), respectively. here evaluated robustness the by implementing them a self‐assembled FRET system using cells expressing different constructs. For with larger signal‐to‐noise ( S/N ) ratio (>9), obtained consistent efficiency E values total concentration R C acceptor to donor for all...
Abstract We present a new fitting method that can robustly and accurately fit the complex transmission curve of superconducting resonator in nonlinear regime. This takes into account varying internal current at different frequencies dependence inductance on <?CDATA $L = L_{0}(1+I^2/I_{*}^{2})$?> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" overflow="scroll"> <mml:mi>L</mml:mi> <mml:mo>=</mml:mo> <mml:msub> <mml:mrow> <mml:mn>0</mml:mn> </mml:mrow> </mml:msub> <mml:mo...
Summary Simultaneous spectral unmixing of excitation and emission spectra ( ExEm unmixing) has inherent ability resolving crosstalks, two key issues quantitative fluorescence resonance energy transfer (FRET) measurement, both the between donor acceptor without additional corrections. We here set up a filter‐based multichannel wide‐field microscope for unmixing‐based FRET imaging m ‐ ExEm‐spFRET ) containing constant system correction factor f sc stable system. performed m‐ExEm‐spFRET with...
Mitochondrial fission regulates mitochondrial function and morphology, has been linked to apoptosis. The factor (Mff), a tail-anchored membrane protein, induces excessive fission, contributing dysfunction Here, we evaluated the inhibitory effect of Bcl-xl, an antiapoptotic on action Mff by using live-cell fluorescence imaging. Microscopic imaging analysis showed that overexpression induced fragmentation apoptosis, which were reversed coexpression Bcl-xl. resonance energy transfer...