A5029745553- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Glycosylation and Glycoproteins Research
- Probiotics and Fermented Foods
- Gut microbiota and health
- Polysaccharides and Plant Cell Walls
- Carbohydrate Chemistry and Synthesis
- Digestive system and related health
- Infant Nutrition and Health
- Enzyme Structure and Function
- Polysaccharides Composition and Applications
- Subcritical and Supercritical Water Processes
- Diet, Metabolism, and Disease
- Food composition and properties
- Neonatal Respiratory Health Research
- Legume Nitrogen Fixing Symbiosis
- Essential Oils and Antimicrobial Activity
- Veterinary medicine and infectious diseases
- Legionella and Acanthamoeba research
- Phytochemistry and Biological Activities
- Cerebrovascular and genetic disorders
- Fibroblast Growth Factor Research
- Toxin Mechanisms and Immunotoxins
- Proteoglycans and glycosaminoglycans research
University of Michigan
2018-2024
University of Gothenburg
2020-2024
Institute for Biomedicine
2024
Polytechnic Institute of Porto
2021-2023
Rede de Química e Tecnologia
2021-2023
Newcastle University
2015-2021
University of Lisbon
2012-2017
Ipca Laboratories (India)
2013-2014
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved synthesis, modification degradation complex carbohydrates. is closely connected with actively curated CAZy database, which provides sequence-based foundation for biochemical, mechanistic structural characterization these diverse proteins. Now celebrating its 10th anniversary online, successful example dynamic,...
Significance Plant cell wall (PCW) polysaccharide degradation is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Ruminococcus flavefaciens synthesizes highly efficient degrading apparatus. Here, six previously unidentified R. CBM families were identified that targeted β-glucans, β-mannans, pectins. Crystal structures of these CBMs revealed recognition β-glucans β-mannans was...
Symbiotic interactions between humans and our communities of resident gut microbes (microbiota) play many roles in health disease. Some bacteria utilize mucus as a nutrient source can under certain conditions damage the protective barrier it forms, increasing disease susceptibility. We investigated how
The study describes a comparative analysis of biochemical, structural and functional properties two recombinant derivatives from Clostridium thermocellum ATCC 27405 belonging to family 43 glycoside hydrolase. hydrolase encoding α-L-arabinofuranosidase (Ct43Araf) displayed an N-terminal catalytic module CtGH43 (903 bp) followed by carbohydrate binding modules CtCBM6A (405 CtCBM6B (402 towards the C-terminal. Ct43Araf its truncated derivative were cloned in pET-vectors, expressed Escherichia...
Functional attributes of a thermostable β-(1→4)-mannanase were investigated from Clostridium thermocellum ATCC 27405. Its sequence comparison the exhibited highest similarity with Man26B C. F1. The full length CtManf and truncated CtManT cloned in pET28a(+) vector expressed E. coli BL21(DE3) cells, exhibiting 53 kDa 38 proteins, respectively. On basis substrate specificity hydrolyzed product profile, classified as β-(1→4)-mannanase. A 1.5 fold higher activity both enzymes was observed by...
Plant biomass is central to the carbon cycle and environmentally sustainable industries exemplified by biofuel sector. cell wall degrading enzymes generally contain noncatalytic carbohydrate binding modules (CBMs) that fulfil a targeting function, which enhances catalysis. CBMs bind β-glucan chains often display broad specificity recognizing β1,4-glucans (cellulose), β1,3-β1,4-mixed linked glucans xyloglucan, β1,4-glucan decorated with α1,6-xylose residues, structures common three...
ABSTRACT In general, cellulases and hemicellulases are modular enzymes in which the catalytic domain is appended to one or more noncatalytic carbohydrate binding modules (CBMs). CBMs, by concentrating parental enzyme at their target polysaccharide, increase capacity of module bind substrate, leading a potentiation catalysis. Clostridium thermocellum hypothetical protein Cthe_0821, defined here as C. Man5A, comprising an N-terminal signal peptide, family 5 glycoside hydrolase (GH5) module, 32...
Breast milk is abundant with functionalized oligosaccharides (MOs) to nourish and protect the neonate. Yet we lack a comprehensive understanding of repertoire evolution MOs across Mammalia. We report ∼400 MO-species associations (>100 novel structures) from glycomics nine mostly understudied species: alpaca, beluga whale, black rhinoceros, bottlenose dolphin, impala, L'Hoest's monkey, pygmy hippopotamus, domestic sheep, striped dolphin. This revealed hitherto unknown existence LacdiNAc motif...
Abstract Symbiotic interactions between humans and our communities of resident gut microbes (microbiota) play many roles in health disease. Some bacteria utilize mucus as a nutrient source can under certain conditions damage the protective barrier it forms, increasing disease susceptibility. We investigated how Ruminococcus torques— known mucin-degrader that remains poorly studied despite its implication inflammatory bowel diseases (IBDs)— degrades mucin glycoproteins or their component O...
Sulfated host glycans (mucin O-glycans and glycosaminoglycans [GAGs]) are critical nutrient sources colonisation factors for Bacteroidetes of the human gut microbiota (HGM); a complex ecosystem comprising essential microorganisms that coevolved with humans to serve important roles in pathogen protection, immune signalling, nutrition. Carbohydrate sulfatases enzymes access sulfated capable exquisite regio- stereo-selective substrate recognition. In these enzymes, common recognition features...
Abstract Breast milk is abundant with functionalized oligosaccharides (MOs), to nourish and protect the neonate. Yet we lack a comprehensive understanding of repertoire evolution MOs across Mammalia. We report ∼400 MO-species associations (>100 novel structures) from glycomics nine mostly understudied species: alpaca, beluga whale, black rhinoceros, bottlenose dolphin, impala, L’Hoest’s monkey, pygmy hippopotamus, domestic sheep, striped dolphin. This revealed hitherto unknown existence...
This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with an engineered His6 tag and expressed Escherichia coli BL21 (DE3) cells. A homogenous 15 kDa protein purified by immobilized metal ion chromatography (IMAC). Ligand binding analysis carried out affinity electrophoresis using various soluble ligands. showed a manno-configured ligand specific displaying significant association konjac...