A5053933323- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA regulation and disease
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- DNA and Nucleic Acid Chemistry
- Viral Infections and Immunology Research
- Biochemical and Molecular Research
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Genomic variations and chromosomal abnormalities
- Virus-based gene therapy research
- Peptidase Inhibition and Analysis
- Nuclear Structure and Function
- Enzyme Structure and Function
- Hippo pathway signaling and YAP/TAZ
- Hemoglobin structure and function
- Zebrafish Biomedical Research Applications
- Molecular Biology Techniques and Applications
- Chromatin Remodeling and Cancer
- Genetics, Aging, and Longevity in Model Organisms
- Viral Infectious Diseases and Gene Expression in Insects
Cleveland Clinic
2012-2023
Cleveland Clinic Lerner College of Medicine
2011-2022
Case Western Reserve University
2019-2021
Case Comprehensive Cancer Center
2002-2009
The University of Texas Southwestern Medical Center
1989-1995
University of Texas Health Science Center at Dallas
1986
Massachusetts Institute of Technology
1983-1985
Center for Cancer Research
1984-1985
Stanford University
1979-1982
Salk Institute for Biological Studies
1982
The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
Mutant Syrian hamster cells resistant to N-(phosphonacetyl)-L-aspartate (PALA), a transition state analog inhibitor of aspartate transcarbamylase, overproduce CAD, multifunctional protein which catalyzes the first three reactions de novo UMP biosynthesis. Increased levels single mRNA cause overproduction CAD in all PALA-resistant mutants examined thus far. A recombinant plasmid containing 2,3-kilobase insert complementary 3'-proximal region this 7.9-kilobase has been prepared and used show...
The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5′ end intervening sequence joined to a site near 3′ splice site. This lariat structure, which been characterized for adenovirus 2 major late transcript, branch point, with 2′-5′ and 3′-5′ phosphodiester bonds emanating from single adenosine residue. excised retains terminates guanosine residue hydroxyl group. phosphate group at junction between two exons originates precursor.
Minor RNA splicing defects can cause a major human developmental disorder.
A conserved sequence element in a minor class of eukaryotic pre-messenger RNA (pre-mRNA) introns was previously proposed to base pair with complementary the U12 small nuclear (snRNA) manner analogous pairing U2 snRNA branch site major introns. Here, mutations generated this block splicing member intron vivo. This relieved by coexpression containing compensatory that restore interaction. These results show pre-mRNA is distinct existing alongside animal genomes, and these also establish an...
A soluble whole-cell extract prepared accurately from HeLa cells splices 2-3% of the RNA transcribed a DNA template containing first and second leader exons late adenovirus RNA. The spliced was detected by sensitive technique using hybridization to single-stranded phage M13 cDNA clone, followed binding nitrocellulose filters. identity established RNase T1 pancreatic two-dimensional peptide mapping. bond formed during in vitro splicing reaction appears be typical 3',5'-phosphodiester as...