A5020573245- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Plant and Fungal Interactions Research
- DNA and Nucleic Acid Chemistry
- Stress Responses and Cortisol
- Plant Disease Resistance and Genetics
- Hepatitis B Virus Studies
- RNA regulation and disease
- Bacterial Genetics and Biotechnology
- Acute Myeloid Leukemia Research
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Neuroendocrine regulation and behavior
- Viral gastroenteritis research and epidemiology
- Parasitic Infections and Diagnostics
- Hybrid Renewable Energy Systems
- Hepatitis C virus research
- Cardiovascular and exercise physiology
- Nuclear Structure and Function
- Social Issues in Poland
- Exercise and Physiological Responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
Polish Academy of Sciences
1981-2024
Public Agenda
2024
University of Warsaw
2019
Central Institute for Labour Protection
2015
Rockefeller University
2004-2014
Albert Einstein College of Medicine
2005
Washington University in St. Louis
1992
Massachusetts Institute of Technology
1984-1990
Center for Cancer Research
1984-1990
University of Virginia
1989-1990
The Hippo pathway was initially discovered in Drosophila melanogaster as a key regulator of tissue growth. It is an evolutionarily conserved signaling cascade regulating numerous biological processes, including cell growth and fate decision, organ size ...Read More
The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5′ end intervening sequence joined to a site near 3′ splice site. This lariat structure, which been characterized for adenovirus 2 major late transcript, branch point, with 2′-5′ and 3′-5′ phosphodiester bonds emanating from single adenosine residue. excised retains terminates guanosine residue hydroxyl group. phosphate group at junction between two exons originates precursor.
Splicing complexes that form a rabbit beta-globin precursor mRNA (pre-mRNA) have been analyzed for their small nuclear RNA (snRNA) content by both affinity chromatography and specific probe hybridization of replicas native electrophoretic gels. A pathway spliceosome assembly was deduced has at least three stages. (i) U2 ribonucleoprotein (snRNP) alone binds to sequences upstream the 3' splice site. (ii) U4, U5, U6 snRNPs bind, apparently simultaneously. (iii) U4 snRNP is released generate...
We have analyzed the pathway of mammalian spliceosome assembly in vitro using a mobility retardation assay. The binding splicing complexes to both wild-type and mutant beta-globin pre-RNAs was studied. Three kinetically related, ATP-dependent complexes, alpha, beta, gamma, were resolved with substrate. These formed, temporally order decreasing mobility, alpha----beta----gamma. All three contained U2 snRNA. RNA intermediates splicing, i.e., free 5' exon intron lariat + 3' exon, found...
Transcription elongation by RNA polymerase II (RNAPII) is negatively regulated the human factors DRB-sensitivity inducing factor (DSIF) and negative (NELF). A 66-kilodalton subunit of NELF (NELF-A) shows limited sequence similarity to hepatitis delta antigen (HDAg), viral protein required for replication virus (HDV). The host RNAPII has been implicated in HDV replication, but detailed mechanism role HDAg this process are not understood. We show that binds directly stimulates transcription...
Extracts of wheat germ contain a RNA ligase activity that catalyzes the conversion linear polyribonucleotides into covalently closed circles. As reported previously, this enzyme joins two ends substrate via 2'-phosphomonoester, 3',5'-phosphodiester linkage. In present work we provide evidence 2',3'-cyclic phosphate group at 3' terminus is required for ligation and 5'-hydroxyl end phosphorylated before are joined. We report on presence polynucleotide kinase 3'-phosphodiesterase activities in...
Miroslawa Siatecka, José L. Reyes, and Maria M. Konarska The Rockefeller University, New York, York 10021 USA
Mutants of the rabbit beta-globin gene lacking natural site branch formation in second intervening sequence have been analyzed for vitro splicing activity. RNAs transcribed from these mutants were spliced, via lariat formation, at a reduced rate compared to wild-type RNA. The sites mapped by direct RNA analysis and primer-extension analysis. sequences three examined did not conform previously determined consensus sequence, nor 5' splice complementary.
HeLa cell extract contains RNA ligase activity that converts linear polyribonucleotides to covalently closed circles. substrates containing 2′,3′-cyclic phosphate and 5′-hydroxyl termini are circularized by formation of a normal 3′,5′ phosphodiester bond. This differs from previously described wheat germ which circularizes molecules with 5′ ends 2′-phosphomonoester, 3′,5′-phosphodiester linkage (Konarska et al., Nature 293, 112–116, 1981; Proc. Natl. Acad. Sci. USA 79, 1471–1478, 1982). The...
The sequence of the 5′‐terminal leader fragment preceding AUG codon in RNA tobacco mosaic virus (TMV), tomato strain, SPS isolate, has been determined. This RNA, similarly to RNAs U1 and Dahlemense strains TMV [Kukla et al. (1979) Eur. J. Biochem. 98 , 61–66] 7‐methylguanosine(5′)triphospho(5′)guanosine cap separated from initiation by a long stretch nucleotides devoid guanosine residues. RNase‐T 1 ‐resistant 73‐nucleotide‐long isolate was assayed for its ability interact with eukaryotic...