A5016666139- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Drug Transport and Resistance Mechanisms
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Transgenic Plants and Applications
- Plant Pathogenic Bacteria Studies
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Antimicrobial Resistance in Staphylococcus
- Radioactive Decay and Measurement Techniques
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Reactions
- Biochemical and Structural Characterization
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Metal complexes synthesis and properties
- Antifungal resistance and susceptibility
- Trace Elements in Health
- Mass Spectrometry Techniques and Applications
- Phenothiazines and Benzothiazines Synthesis and Activities
ZHAW Zurich University of Applied Sciences
2023-2025
ETH Zurich
2008-2015
Institute of Molecular Biology and Biophysics
2013
University of California, San Francisco
2006
Transport across cellular membranes is an essential process that catalyzed by diverse membrane transport proteins. The turnover rates of certain transporters are inhibited their substrates in a termed trans-inhibition, whose structural basis poorly understood. We present the crystal structure molybdate/tungstate ABC transporter (ModBC) from Methanosarcina acetivorans trans-inhibited state. regulatory domains nucleotide-binding subunits close contact and provide two oxyanion binding pockets...
N-Linked glycosylation is an essential post-translational protein modification in the eukaryotic cell. The initial transfer of oligosaccharide from a lipid carrier onto asparagine residues within consensus sequon catalyzed by oligosaccharyltransferase (OST). first X-ray structure complete bacterial OST enzyme, Campylobacter lari PglB, was recently determined. To understand mechanism we have quantified binding and turnover vitro using purified enzyme fluorescently labeled, synthetic peptide...
Analyzing purified membrane proteins and protein complexes by mass spectrometry has been notoriously challenging required highly specialized buffer conditions, sample preparation methods, apparatus. Here we show that a standard matrix-assisted laser desorption/ionization (MALDI) protocol, if used in combination with high-mass detector, allows straightforward spectrometric measurements of integral their complexes, directly following purification detergent solution. Molecular weights can be...
Abrin is a highly toxic plant protein encompassing four isoforms, abrin-a, -b, -c and -d. An abrin reference material was isolated from Abrus precatorius certified (EURM-113) by the EuroBioTox consortium. Here, we present detailed characterisation of N-glycosylation profile EURM-113. The monosaccharide composition N-glycans determined quantified. Release yielded 13 different partially xylosylated, oligomannosidic paucimannosidic glycan structures. Two were found at N82 N110 abrin-b A-chain...
Asparagine-linked glycosylation is a post-translational protein modification that conserved in all domains of life. The initial transfer lipid-linked oligosaccharide (LLO) onto acceptor asparagines catalyzed by the integral membrane oligosaccharyltransferase (OST). previously reported structure single-subunit OST enzyme, Campylobacter lari PglB, revealed partially disordered external loop (EL5), whose role catalysis was unclear. We identified new and functionally important sequence motif EL5...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIon Exchange Resin-Catalyzed Esterification of Salicylic Acid with MethanolM. B. Bochner, S. M. Gerber, W. R. Vieth, and A. J. RodgerCite this: Ind. Eng. Chem. Fundamen. 1965, 4, 3, 314–317Publication Date (Print):August 1, 1965Publication History Published online1 May 2002Published inissue 1 August 1965https://pubs.acs.org/doi/10.1021/i160015a013https://doi.org/10.1021/i160015a013research-articleACS PublicationsRequest reuse permissionsArticle...
A certified reference material of ricin (CRM-LS-1) was produced by the EuroBioTox consortium to standardise analysis this biotoxin. This study established N-glycan structures and proportions including their loci occupancy CRM-LS-1. The glycan profile compared with from different preparations other cultivars isoforms. total 15 oligomannosidic or paucimannosidic were identified in Paucimannose mainly found within A-chain oligomannose constituted major type B-chain. Furthermore, novel primary...
Most Staphylococcus aureus isolates produce either a serotype 5 (CP5) or 8 (CP8) capsular polysaccharide, and the CP antigens are targets for vaccine development. Since CP5 CP8 have similar trisaccharide repeating units, it is important to identify an epitope shared by both CP8. To characterize cross-reactivity between CP8, immunogenicity of conjugate vaccines in mice rabbits was evaluated serological assays. Immune sera were also tested functional activity vitro opsonophagocytic-killing...
We have previously presented a straightforward approach based on high-mass matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) to study membrane proteins. In addition, the stoichiometry of integral protein complexes could be determined by MALDI-MS, following chemical cross-linking via glutaraldehyde. However, glutaraldehyde polymerizes in solution and reacts nonspecifically with various functional groups proteins, limiting its usefulness for structural studies...