A5100322115- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Escherichia coli research studies
- Lipid Membrane Structure and Behavior
- Caveolin-1 and cellular processes
- Salmonella and Campylobacter epidemiology
- Molecular Biology Techniques and Applications
- Insect and Pesticide Research
- RNA modifications and cancer
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Cervical Cancer and HPV Research
- Antibiotic Resistance in Bacteria
- Protein Structure and Dynamics
Institut National des Sciences Appliquées de Toulouse
2022-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2023
Centre National de la Recherche Scientifique
2022-2023
Liaocheng University
2017
Karamay Central Hospital of Xinjiang
2014
ETH Zurich
2014
Hubei University
2009
Northeast Forestry University
2009
Rutgers, The State University of New Jersey
1995
Using a series of position and nucleotide variants the SV40 late polyadenylation signal we have demonstrated that three sequence elements determine precise site 3′-end cleavage in mammalian premRNAs: an upstream AAUAAA element, downstream U-rich element consisting five nucleotides, at least four which are uridine, preference order A>U> C>> G. Cleavage occurs no closer than 11 bases, but farther 23 bases from element. The is usually located 10–30 site. relative define approximate region...
Abstract mRNA sits at the crossroads of transcription, translation and degradation. Many questions remain about coupling these three processes in Escherichia coli and, particular, how may have an effect on degradation transcription. To characterize interplay between while accounting for we altered initiation or elongation measured effects stability concentration. Using a mapping method, analysed concentration local scale all along transcript. We showed that decrease efficiency destabilizes...
A set of 41 synthetic 5'UTRs with different theoretical translation initiation rates were generated to explore the role in regulation protein levels Escherichia coli. The roles regulating expression reporter genes analyzed vivo. Protein varied substantially between constructs but for most 5'UTRs, not correlated rates. Large variations mRNA concentrations measured even though same concentration transcription inducer was used each case. also found strongly affect stability, and these changes...
This study reports that Escherichia coli phosphatidylcholine-positive (PC + ) strain Top10/ptac66 PE ), in which borrelial PC synthase (PCS) directly condenses exogenous choline with CDP-diacylglycerol (CDP-DAG) to form PC, displayed not only stronger resistance antimicrobial peptides cecropin P1 and indolicidin, but also decreased ability attract macrophages the abdominal cavity of infected mice 36 h following infection, compared control strainTop10/ptac85 – ). Rabbit sera raised against...
We have previously presented a straightforward approach based on high-mass matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) to study membrane proteins. In addition, the stoichiometry of integral protein complexes could be determined by MALDI-MS, following chemical cross-linking via glutaraldehyde. However, glutaraldehyde polymerizes in solution and reacts nonspecifically with various functional groups proteins, limiting its usefulness for structural studies...
The increased serum survival gene (iss) has been identified as a virulence trait associated with the of Escherichia coli, causing colibacillosis in poultry. However, it remains unclear to whether iss mRNA copy number and sequence affect virulence. To examine these influences, we assessed presence iss, analysis, number, resistance. was detected 88 (all) E. coli isolates from different sources, sequencing 16 alleles (32 loci) 10 amino acid sequences (10 loci). Nested polymerase chain reaction...
Objective The current study aimed to investigate the molecular basis of cervical cancer development using a microarray identify differentially expressed genes. This also detect apoptosis genes and proteins find those most aberrantly in explore cause Uighur cancer. Methods An analysis gene expression profiles obtained from cases was performed. Total RNA prepared 10 samples carcinoma normal cervix hybridized Affymetrix oligonucleotide microarrays with probe sets complementary more than 20,000...