A5085303036- Neurobiology and Insect Physiology Research
- Cystic Fibrosis Research Advances
- Physiological and biochemical adaptations
- Inhalation and Respiratory Drug Delivery
- Asthma and respiratory diseases
- Insect and Arachnid Ecology and Behavior
- Insect-Plant Interactions and Control
- Trypanosoma species research and implications
- Insect Utilization and Effects
- Tracheal and airway disorders
- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Insect symbiosis and bacterial influences
- Advanced X-ray Imaging Techniques
- Pediatric health and respiratory diseases
- Neuroscience of respiration and sleep
- Microwave-Assisted Synthesis and Applications
- Quinazolinone synthesis and applications
- Advanced MRI Techniques and Applications
- Environmental Toxicology and Ecotoxicology
- Synthesis and biological activity
- Ion channel regulation and function
- Insect and Pesticide Research
- Respiratory and Cough-Related Research
- Pain Mechanisms and Treatments
Czech Academy of Sciences, Institute of Physiology
2017-2024
Respiratory Clinical Trials
2024
University of Saskatchewan
2013-2023
Saskatchewan Health
2017
University of Toronto
2008-2009
McGill University
2007-2008
McMaster University
2001-2006
Fundación Ciencias Exactas y Naturales
1998
Pulmonary ionocytes are a newly discovered airway epithelial cell type proposed to be major contributor cystic fibrosis (CF) lung disease based on observations they express the transmembrane conductance regulator (CFTR) anion channel at higher level than any other in epithelia. Moreover, genetically manipulated experimental models that lack develop NaCl transport abnormalities and surface liquid (ASL) dehydration consistent with CF. However, no direct evidence indicates engage or contribute...
Cystic fibrosis (CF) is caused by dysfunction of the CF transmembrane conductance regulator (CFTR), an anion channel whose leads to chronic bacterial and fungal airway infections via a pathophysiological cascade that incompletely understood. Airway glands, which produce most mucus, do so in response both acetylcholine (ACh) vasoactive intestinal peptide (VIP). glands fail secrete mucus VIP, but ACh. Because vagal cholinergic pathways still elicit strong gland secretion subjects, it unclear...
Mechanisms of ion transport during primary urine formation by the Malpighian tubule Drosophila melanogaster were analyzed through measurements fluid secretion rate, transepithelial flux, basolateral membrane potential (V(bl)) and intracellular activities K(+) (a(K)(i)) Cl(-) (a(Cl)(i)). Calculation electrochemical potentials for both ions permitted assessment possible contributions channels, Na(+):K(+):2Cl(-) cotransport, K(+):Cl(-) to net across membrane. The data show that passive movement...
Submucosal glands line the cartilaginous airways and produce most of antimicrobial mucus that keeps sterile. The are defective in cystic fibrosis (CF), but how this impacts airway health remains uncertain. Although CF mouse strains exhibit mild defects, those with C57Bl/6 genetic background have increased pathology susceptibility to Pseudomonas . Thus, they offer possibility studying whether, if so how, abnormal submucosal gland function contributes disease. We used optical methods study...
Blood-sucking insects experience thermal stress at each feeding event on endothermic vertebrates. We used thermography to examine how kissing-bugs Rhodnius prolixus actively protect themselves from overheating. During feeding, these bugs sequester and dissipate the excess heat in their heads while maintaining an abdominal temperature close ambient. employed a functional-morphological approach, combining histology, µCT X-ray-synchrotron imaging shed light way manage flow of across bodies. The...
The airway mucosal microenvironment is crucial for host defense against inhaled pathogens but remains poorly understood. We report here that the surface normally undergoes surprisingly large excursions in pH during breathing can reach 9.0 inhalation, making it most alkaline fluid body. Transient alkalinization requires luminal bicarbonate and membrane-bound carbonic anhydrase 12 (CA12) antimicrobial. Luminal concentration CA12 expression are both reduced cystic fibrosis (CF), mucus...
The electrophysiological and ion-transporting properties of cultured gill epithelia from freshwater (FW) rainbow trout were examined in the presence cortisol prolactin as media supplements. Epithelia double-seeded insert (DSI) type containing both pavement cells (PVCs) mitochondria-rich (MRCs) grown Leibovitz's L15 on filters allowing exposure to different apical conditions. Experiments carried out two series after 7-9 days symmetrical (L15 apical-L15 basolateral) culture. In series, 100%...
The ubiquitous bacterium Pseudomonas aeruginosa frequently causes hospital-acquired infections. P. also infects the lungs of cystic fibrosis (CF) patients and secretes N-(3-oxo-dodecanoyl)-S-homoserine lactone (3O-C12) to regulate bacterial gene expression critical for persistence. In addition its effects as a quorum-sensing regulator in aeruginosa, 3O-C12 elicits cross-kingdom on host cell signaling leading both pro- or anti-inflammatory effects. We find that these slow mediated through...
Osmotic balance in insects is regulated by the excretory system, consisting of Malpighian tubules and gut under control diuretic antidiuretic factors. Terrestrial must conserve water, antidiuresis norm, only interrupted brief periods. Surprisingly, little known about insects. Two strategies have been described. The first mechanism involves reabsorption fluid from primary urine hindgut. More recently, a second strategy was reported, inhibition formation tubules. Recently, we isolated,...
Cystic fibrosis (CF) is an autosomal recessive genetic disorder caused by mutations in the gene encoding for anion channel cystic transmembrane conductance regulator (CFTR). Several organs are affected CF, but most of morbidity and mortality comes from lung disease. Recent data show that initial consequence CFTR mutation failure to eradicate bacteria before development inflammation airway remodeling. Bacterial clearance depends on a layer surface liquid (ASL) consisting both mucus traps,...
Diabetes mellitus is associated with sensory abnormalities, including exacerbated responses to painful (hyperalgesia) or non-painful (allodynia) stimuli. These abnormalities are symptoms of diabetic peripheral neuropathy (DPN), which the most common complication that affects approximately 50% patients. Yet, underlying mechanisms linking hyperglycemia and DPN remain poorly understood. The transient receptor potential vanilloid 1 (TRPV1) channel plays a central role in such shows elevated...
Abstract Cystic fibrosis is caused by mutations in the gene encoding cystic transmembrane conductance regulator (CFTR) channel, which can result chronic lung disease. The sequence of events leading to disease not fully understood but recent data show that critical pathogenic event loss ability clear bacteria due abnormal airway surface liquid secretion (ASL). However, whether inhalation triggers ASL and this has never been tested. Here we show, using a novel synchrotron-based vivo imaging...
SUMMARY Intracellular ion activities (aion) and basolateral membrane potential (Vbl) were measured in Malpighian tubule cells of Rhodnius prolixus using double-barrelled ion-selective microelectrodes. In saline containing 103mmoll-1Na+, 6mmoll-1 K+ 93mmoll-1Cl-, intracellular unstimulated upper tubules 21, 86 32mmoll-1, respectively. serotonin-stimulated tubules, aCl was unchanged, whereas aNa increased to 33mmoll-1 aK declined 71mmoll-1. Vbl -59mV -63mV for stimulated Calculated...
The effects of changes in the salinity rearing medium on Malpighian tubule fluid secretion and ion transport were examined larvae freshwater mosquito Aedes aegypti saltwater species Ochlerotatus taeniorhynchus. For unstimulated tubules both species, K+ concentration secreted was significantly lower when reared 30% or 100% seawater (O. taeniorhynchus only), relative to from freshwater‐reared larvae. Na+ O. higher results suggest that larval lead sustained mechanisms tubules. Furthermore,...
Rhodnius prolixus is a hematophagous insect vector of Chagas disease capable ingesting up to 10 times its unfed body weight in blood single meal. The excess water and ions ingested with the meal are expelled through rapid postprandial diuresis driven by Malpighian tubules. Diuresis triggered at least two diuretic hormones, CRF-related peptide serotonin, which were traditionally believed trigger cAMP as an intracellular second messenger. Recently, calcium has been suggested act messenger...
Measurements of intracellular and luminal ion activities as well membrane potential were used to calculate electrochemical gradients for Cl-, Na+, K+ H+ across the apical during fluid secretion by Malpighian tubules Rhodnius prolixus. The results show that contribution Na+/H+ and/or K+/H+ exchangers is feasible both in unstimulated serotonin-stimulated tubules. Similarly, Cl- consistent with passive movement from cell lumen through channels. K+:Cl- cotransport paracellular net...
The airway is kept sterile by an efficient innate defense mechanism. cornerstone of mucus containing diverse antimicrobial factors that kill or inactivate pathogens. Most the in upper airways secreted submucosal glands. In patients with cystic fibrosis (CF), fails and lungs are colonized bacteria, usually Pseudomonas aeruginosa . Accumulating evidence suggests glands contribute to CF pathogenesis failing respond appropriately inhalation bacteria. However, regulation immune system remains...
We examined the effects of bathing saline Na+/K+ ratio, bumetanide and hydrochlorothiazide on fluid ion transport by serotonin-stimulated Malpighian tubules Rhodnius prolixus. Previous pharmacological electrophysiological studies indicate that a bumetanide-sensitive Na+/K+/2Cl- cotransporter is primary route for basolateral entry into cell during secretion. The goal this study was to resolve apparent conflict between relatively high secretion rates bathed in K+-free evidence cotransporters...
Given that hematophagous insects ingest large quantities of blood in a single meal, they must undergo rapid post-prandial diuresis order to maintain homeostasis. In the kissing bug Rhodnius prolixus (Hemiptera: Reduviidae), coordinated activity Malpighian tubules and anterior midgut maintains water ion balance during diuresis. Three four hours after meal diuretic process finishes, animal enters an antidiuretic state ensure conservation until next intake. The processes are tightly regulated...
Insect haemolymph typically contains very high levels of free amino acids. This study shows that acids can modulate the secretion ions and water by isolated Malpighian tubules Rhodnius prolixus Drosophila melanogaster. Secretion rates in amino-acid-free saline increase after addition serotonin to a peak value, then slowly decline plateau. Addition glutamine, glutamate or aspartate such increases dramatically relative controls saline, these are sustained for 1-2 h. Seven other have more...