A5058383517- Hearing, Cochlea, Tinnitus, Genetics
- Neurogenesis and neuroplasticity mechanisms
- Hearing Loss and Rehabilitation
- Molecular Biology Techniques and Applications
- Vestibular and auditory disorders
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Nerve injury and regeneration
- Electric and Hybrid Vehicle Technologies
- Cancer-related molecular mechanisms research
- Real-time simulation and control systems
- Hydraulic and Pneumatic Systems
- RNA modifications and cancer
- Industrial Technology and Control Systems
- Acoustic Wave Phenomena Research
- Circular RNAs in diseases
- Cellular Mechanics and Interactions
- Biochemical Analysis and Sensing Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Plant Molecular Biology Research
- Bacteriophages and microbial interactions
- Connexins and lens biology
- Neuroscience of respiration and sleep
Model Animal Research Center
2017-2025
Nanjing University
2017-2025
Nanjing Drum Tower Hospital
2025
Zunyi Medical University
2024
Beth Israel Deaconess Medical Center
2024
State Key Laboratory of Pharmaceutical Biotechnology
2022-2024
CRRC (China)
2024
Nanjing Brain Hospital
2020-2022
Sinopec (China)
2013-2020
University of Michigan
2014-2019
Neurotrophin-3 (Ntf3) and brain derived neurotrophic factor (Bdnf) are critical for sensory neuron survival establishment of neuronal projections to epithelia in the embryonic inner ear, but their postnatal functions remain poorly understood. Using cell-specific inducible gene recombination mice we found that, Bbnf Ntf3 required formation maintenance hair cell ribbon synapses vestibular cochlear epithelia, respectively. We also show that supporting cells these key endogenous source...
Abstract Hidden hearing loss (HHL) is a recently described auditory neuropathy believed to contribute speech discrimination and intelligibility deficits in people with normal audiological tests. Animals humans HHL have thresholds but defective cochlear neurotransmission, that is, reduced suprathreshold amplitude of the sound-evoked nerve compound action potential. Currently, only cellular mechanism known for inner hair cell synapses (synaptopathy). Here we report transient Schwann cells...
Astrocytes play many important functions in response to spinal cord injury (SCI) an activated manner, including clearance of necrotic tissue, formation protective barrier, maintenance microenvironment balance, interaction with immune cells, and the glial scar. More more studies have shown that astrocytes are heterogeneous, such as inflammatory astrocyte 1 (A1) neuroprotective 2 (A2) types. However, subtypes resulting from SCI not been clearly defined. In this study, using single-cell RNA...
Abstract Thirst plays a vital role in the regulation of body fluid homeostasis and if deregulated can be life-threatening. Interoceptive neurons subfornical organ (SFO) are intrinsically osmosensitive their activation by hyperosmolarity is necessary sufficient for generating thirst. However, primary molecules sensing systemic osmolarity these remain elusive. Here we show that mechanosensitive TMEM63B cation channel osmosensor required interoceptive to drive highly expressed excitatory SFO...
Hypotonic stress causes the activation of swelling-activated nonselective cation channels (NSCCs), which leads to Ca2+-dependent regulatory volume decrease (RVD) and adaptive maintenance cell volume; however, molecular identities osmosensitive NSCCs remain unclear. Here, we identified TMEM63B as an NSCC activated by hypotonic stress. is enriched in inner ear sensory hair cells. Genetic deletion results necroptosis outer cells (OHCs) progressive hearing loss. Mechanistically, channel mediates...
MicroRNAs are small noncoding RNAs that serve as important regulators of eukaryotic gene expression and emerging novel diagnostic therapeutic targets for human diseases. Robust reliable detection miRNAs is an essential step understanding the functional significance these in both physiological pathological processes. Existing methods miRNA quantification rely on fluorescent probes optimal specificity. In this study, we developed a high-performance real-time reverse transcriptase-polymerase...
Significance The auditory sensory epithelium contains two major cell types: hair cells and supporting cells. Mammalian do not regenerate after damage or loss, resulting in permanent hearing impairment. How loss affects function remains to be determined. Here, we demonstrate that inner border phalangeal cells, the types of surrounding can replenished completely selective ablation neonatal cochlea, allowing preserved. Our findings challenge view mammalian has limited intrinsic regenerative...
Cisplatin can cause irreversible hearing loss. However, effective approaches to its prevention are not established. In this study, the effect of traditional Chinese medicine monomer pinoresinol diglucoside (PDG) is evaluated on cisplatin-induced ototoxicity and underlying mechanism action. PDG significantly increases cell viability inhibits reactive oxygen species production ferroptosis in cisplatin-treated House Ear Institute-Organ Corti 1 cells basilar membranes. partially restores loss...
Dentate gyrus (DG) development requires specification of granule cell (GC) progenitors in the hippocampal neuroepithelium, as well their proliferation and migration into primordial DG. We identify Plexin family members Plxna2 Plxna4 important regulators DG development. Distribution immature GCs is regulated by Sema5A signaling through PlxnA2 a functional GTPase-activating protein (GAP) domain Rap1 small GTPases. In adult Plxna2−/− but not Plxna2-GAP-deficient mice, dentate GC layer severely...
Abstract Background Gene regulation at transcript level can provide a good indication of the complex signaling mechanisms underlying physiological and pathological processes. Transcriptomic methods such as microarray quantitative real-time PCR require stable reference genes for accurate normalization gene expression. Some but not all studies have shown that housekeeping (HGKs), β-actin (ACTB) glyceraldehyde-3-phosphate dehydrogenase (GAPDH), which are routinely used normalization, may vary...
Producing hair cells of the inner ear is major goal ongoing research that combines advances in developmental and stem cell biology. The recent advent an organoid protocol—resulting three-dimensional cell-derived tissues resembling vestibular sensory epithelia—has sparked interest applications such as regeneration, drug discovery, disease modeling. In this study, we adapted protocol for a novel mouse embryonic line with fluorescent reporter Pax2 expression. We used Pax2EGFP/+ formation to...
Cingulin (CGN) is a cytoskeleton-associated protein localized at the apical junctions of epithelial cells. CGN interacts with major cytoskeletal filaments and regulates RhoA activity. However, physiological roles in development human diseases are currently unknown. Here, we report multi-generation family presenting autosomal dominant non-syndromic hearing loss (ADNSHL) that co-segregates heterozygous truncating variant, c.3330delG (p.Leu1110Leufs*17). normally expressed cell organ Corti,...
Increasing studies have revealed that long noncoding RNAs (lncRNAs) are not transcriptional noise but play important roles in the regulation of a wide range biological processes, and dysregulation lncRNA genes is associated with disease development. Alzheimer’s (AD) chronic neurodegenerative usually starts slowly gets worse over time. However, little known about AD how transcriptionally regulated. Herein, we analyzed RNA-seq data ChIP-seq histone modification from CK-p25 model control mice...
Animal-based studies have provided important insights into the structural and functional consequences of noise exposure on cochlea. Yet, less is known about molecular mechanisms by which induces cochlear damage, particularly at relatively low levels. While there ample evidence that leads to changes in inner ear metabolism, specific effects metabolome are poorly understood. In this study we applied liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS)-based metabolomics analyze...
Aged related decline of inner ear function contributes to both hearing loss and balance disorders, which lead impaired quality life falls that can result in injury even death. The cellular mechanisms responsible for the ear's functional have been controversial, but hair cell has considered key cause a long time. However, recent studies showed cochlea, synapses precedes or neuronal loss, this synaptopathy is an early step decline. Whether similar process occurs vestibular organ, its timing...
TMEM63B is a mechanosensitive cation channel activated by hypoosmotic stress and mechanic stimulation. We recently reported brain-specific alternative splicing of exon 4 in TMEM63B. The short variant lacking 4, which constitutes the major isoform brain, exhibits enhanced responses to stimulation compared long containing 4. However, mechanisms affecting this differential response are unclear. Here, we showed that exhibited stronger cell surface expression variant. Using mutagenesis screening...