A5018333261- Biofuel production and bioconversion
- Fungal and yeast genetics research
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- Polysaccharides and Plant Cell Walls
- Fungal Biology and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Enzyme Catalysis and Immobilization
- Circadian rhythm and melatonin
- Microbial Metabolites in Food Biotechnology
- Plant Pathogens and Fungal Diseases
- Plant Gene Expression Analysis
- Insect symbiosis and bacterial influences
- Mycotoxins in Agriculture and Food
- Insect Resistance and Genetics
- Cancer-related gene regulation
- Seaweed-derived Bioactive Compounds
- Catalysis for Biomass Conversion
- Polyamine Metabolism and Applications
- Photoreceptor and optogenetics research
- Food composition and properties
- Enzyme-mediated dye degradation
- Advanced Cellulose Research Studies
- Entomopathogenic Microorganisms in Pest Control
Shandong University
2016-2025
State Key Laboratory of Microbial Technology
2016-2025
Hefei University
2024
Guilin University of Technology
2021-2024
Hong Kong University of Science and Technology
2023
University of Hong Kong
2023
Riverside Hospital of Guangxi Zhuang Autonomous Region
2023
Qingdao Binhai University
2019-2021
Harvard University
2010-2016
Evolutionary Genomics (United States)
2016
Many Penicillium species could produce extracellular enzyme systems with good lignocellulose hydrolysis performance. However, these and their are still poorly understood explored due to the lacking of genetic information. Here, we present genomic secretomic analyses decumbens that has been used in industrial production lignocellulolytic enzymes China for more than fifteen years. Comparative genomics analysis phylogenetically most similar chrysogenum revealed P. evolved genes involved plant...
Filamentous fungus Penicillium oxalicum produces diverse lignocellulolytic enzymes, which are regulated by the combinations of many transcription factors. Here, a single-gene disruptant library for 470 factors was constructed and systematically screened cellulase production. Twenty (including ClrB, CreA, XlnR, Ace1, AmyR, 15 unknown proteins) were identified to play putative roles in activation or repression synthesis. Most these regulators have not been characterized any fungi before. We...
Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome P. JU-A10-T sequenced compared with that wild-type 114-2. Further, transcriptomes secretomes were between strains. Selective hyper-production but not all secreted proteins observed in mutant, making it more specific producer lignocellulolytic enzymes. Functional analysis...
Aims: Improvement of cellulase production Penicillium decumbens by genome shuffling an industrial catabolite‐repression‐resistant strain JU‐A10 with its mutants. Methods and Results: After two rounds shuffling, three fusants, GS2‐15, GS2‐21 GS2‐22, were obtained, showing 100%, 109% 94% increase in FPase activity than respectively. The the fusants on various substrates, such as corn stover, wheat straw, bagasse corncob residue, was studied. maximum productivities GS2‐22 92·15, 102·63 92·35...
As a common neurotransmitter in the nervous system, γ-aminobutyric acid (GABA) modulates locomotory patterns both vertebrates and invertebrates. However, signaling mechanisms underlying behavioral effects of GABAergic modulation are not completely understood. Here, we demonstrate that signal C. elegans amplitude undulatory head bending through extrasynaptic neurotransmission conserved metabotropic receptors. We show RME motor neurons generate activity correlate with causally links amplitude....
Protein ubiquitination is usually coupled with proteasomal degradation and crucial in regulating protein quality. The E3 ubiquitin-protein ligase SCF (Skp1-Cullin-F-box) complex directly recognizes the target substrate via interaction between F-box substrate. determinant of specificity. limited number identified ubiquitin ligase-substrate pairs a major bottleneck field. Penicillium oxalicum contains many transcription factors, such as BrlA, CreA, XlnR, Ace1, conserved filamentous fungi that...
Abstract Background The production of cellulases by filamentous fungi is a crucial aspect sustainable bioproduction from renewable lignocellulosic biomass. Following the transcription cellulase genes in nucleus, complex pathway involving translation, folding, and secretion required to produce extracellular cellulases. Most studies about have focused on examining transcriptional regulatory mechanisms enhancement enzyme gene levels; comparatively, little known protein translation for...
Summary For most organisms, carbon and nitrogen uptake are essential for growth, development and, where applicable, pathogenesis. The role of the catabolite repressor transcription factor homologue BbcreA in entomopathogenic fungus B eauveria bassiana was investigated. Deletion resulted pleiotropic effects, including nutrient toxicity, leading to a novel cell lytic phenotype. Fungal growth rich media minimal containing select amino acids/peptides severely compromised, with microscopic...
Enzymes that degrade lignocellulose to simple sugars are of great interest in research and for biotechnology because their role converting plant biomass fuels chemicals. The synthesis cellulolytic enzymes filamentous fungi is tightly regulated at the transcriptional level, with activator ClrB/CLR-2 playing a critical many species. In Penicillium oxalicum, clrB overexpression could not relieve dependence cellulase expression on cellulose as an inducer, suggesting controlled...
Many Penicillium species produce enzyme systems with good performances in lignocellulose degradation. In our laboratory, lignocellulolytic enzyme-producing oxalicum (formerly classified as decumbens) strains have been studied for more than 30 years. High cellulase-producing mutants obtained through random mutagenesis and genetic engineering, the components elucidated using biology tools. The effects of different carbon sources on production level enzymes studied, related molecular mechanisms...
Cellulolytic enzyme hydrolysis of lignocellulose biomass to release fermentable sugars is one the key steps in biofuel refining. Gene expression fungal cellulolytic enzymes tightly controlled at transcriptional level. Key transcription factors such as activator ClrB/CLR2 and XlnR/XYR1, well repressor CreA/CRE1 play crucial roles this process. The putative protein methyltransferase LaeA/LAE1 has also been reported regulate gene enzyme. formation was compared among Penicillium oxalicum wild...