A5025888661- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Image and Signal Denoising Methods
- ATP Synthase and ATPases Research
- Digital Filter Design and Implementation
- Enzyme Structure and Function
- Advanced X-ray Imaging Techniques
- Advanced Data Compression Techniques
- Advancements in Semiconductor Devices and Circuit Design
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- Parasitic Infections and Diagnostics
- Nicotinic Acetylcholine Receptors Study
- Mitochondrial Function and Pathology
- Electrical and Bioimpedance Tomography
- Biomedical Research and Pathophysiology
- Toxoplasma gondii Research Studies
- Galectins and Cancer Biology
- VLSI and FPGA Design Techniques
- VLSI and Analog Circuit Testing
- Magnetic and Electromagnetic Effects
- Low-power high-performance VLSI design
- Nuclear Structure and Function
Goethe University Frankfurt
2020-2024
Max Planck Institute of Biophysics
2017-2024
Intel (United States)
2021-2023
Instituto de Medicina Tropical “Pedro Kourí”
2014
Pontifical Catholic University of Peru
2012
Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb air-water interface unsupported films aqueous solution, which can result partial or complete denaturation. We investigated yeast fatty acid synthase at by electron cryo-tomography single-particle image processing. Around 90% adsorbed are partly denatured. show that unfolded regions face interface. Denaturation contact with air may happen any stage specimen...
Abstract Pentameric ligand-gated ion channels (pLGICs) of the Cys-loop receptor family are key players in fast signal transduction throughout nervous system. They have been shown to be modulated by lipid environment, however underlying mechanism is not well understood. We report three structures 5-HT 3A serotonin (5HT 3 R) reconstituted into saposin-based bilayer discs: a symmetric and an asymmetric apo state, agonist-bound state. In comparison previously published 5HT R conformations...
Structures of macromolecules in their native state provide unique unambiguous insights into functions. Cryo-electron tomography combined with subtomogram averaging demonstrated the power to solve such structures situ at resolutions range 3 Angstrom for some macromolecules. In order be applicable structural determination majority observable cells limited amounts, processing tomographic data has performed a high-throughput manner. Here we present TomoBEAR-a modular configurable workflow engine...
Cryo-electron tomography combined with subtomogram averaging (StA) has yielded high-resolution structures of macromolecules in their native context. However, StA is not commonplace due to beam-induced sample drift, images poor signal-to-noise ratios (SNR), challenges CTF correction, and limited particle number. Here we address these issues by collecting tilt series a higher electron dose at the zero-degree tilt. Particles interest are then located within reconstructed tomograms, processed...
Nitrate is an abundant nutrient and electron acceptor throughout Earth's biosphere. Virtually all nitrate in nature produced by the oxidation of nitrite oxidoreductase (NXR) multiprotein complex. NXR a crucial enzyme global biological nitrogen cycle, found nitrite-oxidizing bacteria (including comammox organisms), which generate bulk environment, anaerobic ammonium-oxidizing (anammox) produce half dinitrogen gas our atmosphere. However, despite its central role biology decades intense study,...
Secretins form multimeric channels across the outer membrane of Gram-negative bacteria that mediate import or export substrates and/or extrusion type IV pili. The secretin complex Thermus thermophilus is an oligomer 757-residue PilQ protein, essential for DNA uptake and pilus extrusion. Here, we present cryo-EM structure this bifunctional at a resolution ~7 Å using new reconstruction protocol. Thirteen protomers large periplasmic domain six stacked rings in membrane. A homology model protein...
PowerVia Technology [1] is a novel innovation to extend Moore's Law scaling by having Power Delivery on the backside. This paper presents pre & post-silicon findings from implementing an Intel E-Core in Technology. enabled standard cell utilization of >90% large areas core while showing >5% Frequency benefit Silicon due reduced IR drop. Successful Post-Silicon debug demonstrated with slightly higher but acceptable throughput times. The thermal characteristic test-chip line power densities...
ABSTRACT Structures of macromolecules in their native state provide unique unambiguous insights into functions. Cryo-electron tomography combined with subtomogram averaging demonstrated the power to solve such structures situ at resolutions range 3 Angstrom for some macromolecules. In order be applicable structural determination majority observable cells limited amounts, processing tomographic data has performed a high-throughput manner. Here we present TomoBEAR - modular configurable...
ABSTRACT Electron cryo-microscopy analyzes the structure of proteins and protein complexes in vitrified solution. Proteins tend to adsorb air-water interface unsupported films aqueous solution, which can result partial or complete denaturation protein. We investigated yeast fatty acid synthase at by electron cryo-tomography single-particle image processing. Around 90% adsorbed are partly denatured. show that unfolded regions those facing interface. Denaturation contact with air may happen...
The median filter is a non-linear used for removal of salt and pepper noise from images. Each pixel the image replaced by its surrounding elements, value calculated sorting data. complexity algorithms on filters are O(n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) or O(n), depending kernel size. Those were formulated scalar single processor computers, with few them successfully adapted implemented computer parallel architecture. In...
Intestinal parasites are very common infections worldwide and they responsible for significant public health problems.The World Health Organization estimates that one-third of the world population is infected some epidemiologic factors related to transmission have been identified.The purpose this investigation was study prevalence intestinal parasitic in people living rural community "El Canal", Consolación del Sur municipality association with risk factors.All participants were subjected...
Volume alignment is a computationally intensive task. In Subtomogram Averaging (StA) from electron cryotomograms (CryoET), thousands of subtomograms are aligned to reference, which may take hours until days computational time. CryoET datasets contain limited number noisy projections, with very low signal-to-until ratio (SNR). The reference using cross-correlation, an operation that can be optimized when working angle tomograms (LAT), as they sparse in Fourier space. We propose projected...
ABSTRACT Cryo electron tomography (cryo-ET) combined with subtomogram averaging (StA) enables structural determination of macromolecules in their native context. A few structures were reported by StA at resolution higher than 4.5 Å, however all these are from viral proteins or vesicle coats. Reaching high for a broader range samples is uncommon due to beam-induced sample drift, poor signal-to-noise ratio (SNR) images, challenges CTF correction, limited number particles. Here we propose...
This paper describes a novel methodology of creating scan-based at-speed structural patterns to analyze and validate functional critical paths on latch-based high performance CPU cores. Silicon data comparison with traditional transition fault are conducted reported illustrate the effectiveness proposed scheme.